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• W1923103656 endingPage "765" @default.
• W1923103656 startingPage "756" @default.
• W1923103656 abstract "Leptomastix dactylopii (Howard) is an endoparasitoid wasp, natural enemy of mealybug Planococcus citri (Risso). Despite the acquired knowledge regarding this host-parasitoid interaction, only little information is available on the factors of parasitoid origin able to modulate the mealybug physiology. The major alteration observed in P. citri is a strong reduction in fecundity, which is evident soon after parasitization by L. dactylopii or venom injection in unparasitized hosts indicating that this proteinaceus secretion injected at the oviposition plays a key-role in host regulation. Protein identification of L. dactilopii venom has been limited by the lack of literature sources and public protein databases. Here, we identified two venom proteins by an integrated trascriptomic and proteomic approach. A custom-made transcriptomic database from the L. dactylopii venom glands was created by applying the high-throughput RNA sequencing approach. Two-dimensional gel electrophoresis (2DE) trypsinized protein spots were analyzed by high-resolution mass spectrometry (FTICRMS-12 T). The most abundant peptide ions were fragmented by collision induced dissociation and the obtained sequence tags were subjected to custom-made protein database searching. Two putative arginine kinases (full-length and truncated form) were identified. This is the first case in which both, truncated and full length arginine kinases, are identified in an endoparasitoid non-paralyzing venom." @default.
• W1923103656 created "2016-06-24" @default.
• W1923103656 creator A5015464449 @default.
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• W1923103656 date "2015-04-07" @default.
• W1923103656 modified "2023-10-05" @default.
• W1923103656 title "Identification of two arginine kinase forms of endoparasitoid<i>Leptomastix dactylopii</i>venom by bottom up-sequence tag approach" @default.
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• W1923103656 doi "https://doi.org/10.1002/jms.3585" @default.
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• W1923103656 hasPublicationYear "2015" @default.
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