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- W1924962203 abstract "Toll-like Receptors (TLRs) have been shown to mediate the recognition of many types of pathogens and to play an important role in the production of (anti-) inflammatory cytokines, and the initiation of an adaptive immune response. TLR2 plays an important role in the recognition of different components of gram-positive bacteria, mycobacteria, yeast, and schistosomes. To study the role of TLR2 in the immune response, we set about to express the ectodomain of human TLR2 (ecTLR2) with the aim of immunizing mice and generating mAbs directed against human ecTLR2. Expression of ecTLR2 was absent in the yeast P. pastoris and very low in mammalian HEK293 cells. High expression of ecTLR2 was obtained in BL21(DE3) E. coli. However, ecTLR2 expressed in bacteria was insoluble and exclusively present in inclusion bodies. Strategies to facilitate synthesis of ecTLR2 in a soluble form, as well as methods to refold guanidine-HCl-solubilized ecTLR2 protein, were unsuccessful. Thus, attempts to express ecTLR2 in three different expression systems did not yield soluble monomeric TLR2 that could be used for immunization. In this paper, both our efforts to express ecTLR2 and data on the expression of ecTLR2 by other research groups are discussed. Recombinant expression of toll-like receptor 2" @default.
- W1924962203 created "2016-06-24" @default.
- W1924962203 creator A5003425194 @default.
- W1924962203 date "2009-01-01" @default.
- W1924962203 modified "2023-09-26" @default.
- W1924962203 title "Toll-like receptors: tools, assays, and implications for in vitro pyrogen tests" @default.
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