Matches in SemOpenAlex for { <https://semopenalex.org/work/W1925194906> ?p ?o ?g. }
- W1925194906 endingPage "15468" @default.
- W1925194906 startingPage "15449" @default.
- W1925194906 abstract "Hyaluronan (HA) is the main component of the extracellular matrix (ECM). Depending on its chain size, it is generally accepted to exert diverse effects. High molecular weight HA is anti-angiogenic, immunosuppressive and anti-inflammatory, while lower fragments are angiogenic and inflammatory. Human hyaluronidase Hyal-1 (Hyal-1) is one of the main enzymes in the metabolism of HA. This makes Hyal-1 an interesting target. Not only for functional and mechanistic studies, but also for drug development. In this work, Hyal-1 was expressed on the surface of E. coli, by applying Autodisplay, to overcome formation of inactive inclusion bodies. With the cells displaying Hyal-1 an activity assay was performed using stains-all dye. Subsequently, the inhibitory effects of four saponins and 14 plant extracts on the activity of surface displayed Hyal-1 were evaluated. The determined IC50 values were 177 µM for glycyrrhizic acid, 108 µM for gypsophila saponin 2, 371 µM for SA1657 and 296 µM for SA1641. Malvae sylvestris flos, Equiseti herba and Ononidis radix extracts showed IC50 values between 1.4 and 1.7 mg/mL. In summary, Autodisplay enabled the expression of functional human target protein Hyal-1 in E. coli and facilitated an accelerated testing of potential inhibitors." @default.
- W1925194906 created "2016-06-24" @default.
- W1925194906 creator A5007797524 @default.
- W1925194906 creator A5016855923 @default.
- W1925194906 creator A5028837310 @default.
- W1925194906 creator A5068213552 @default.
- W1925194906 creator A5081645354 @default.
- W1925194906 creator A5086288750 @default.
- W1925194906 date "2015-08-26" @default.
- W1925194906 modified "2023-10-16" @default.
- W1925194906 title "Autodisplay of Human Hyaluronidase Hyal-1 on Escherichia coli and Identification of Plant-Derived Enzyme Inhibitors" @default.
- W1925194906 cites W1502367539 @default.
- W1925194906 cites W1542301988 @default.
- W1925194906 cites W1925468999 @default.
- W1925194906 cites W1966119617 @default.
- W1925194906 cites W1966422114 @default.
- W1925194906 cites W1970105862 @default.
- W1925194906 cites W1972625140 @default.
- W1925194906 cites W1975109027 @default.
- W1925194906 cites W1977690808 @default.
- W1925194906 cites W1994806949 @default.
- W1925194906 cites W1997585740 @default.
- W1925194906 cites W1999517789 @default.
- W1925194906 cites W2000525574 @default.
- W1925194906 cites W2003920661 @default.
- W1925194906 cites W2005385797 @default.
- W1925194906 cites W2010427736 @default.
- W1925194906 cites W2012582780 @default.
- W1925194906 cites W2013261821 @default.
- W1925194906 cites W2014132117 @default.
- W1925194906 cites W2016023582 @default.
- W1925194906 cites W2018862317 @default.
- W1925194906 cites W2020487872 @default.
- W1925194906 cites W2027014426 @default.
- W1925194906 cites W2030922028 @default.
- W1925194906 cites W2030997781 @default.
- W1925194906 cites W2033306446 @default.
- W1925194906 cites W2040285608 @default.
- W1925194906 cites W2041014112 @default.
- W1925194906 cites W2048573811 @default.
- W1925194906 cites W2065895195 @default.
- W1925194906 cites W2070070588 @default.
- W1925194906 cites W2070973403 @default.
- W1925194906 cites W2074778310 @default.
- W1925194906 cites W2080606635 @default.
- W1925194906 cites W2086676786 @default.
- W1925194906 cites W2097381450 @default.
- W1925194906 cites W2117925574 @default.
- W1925194906 cites W2120474060 @default.
- W1925194906 cites W2127312900 @default.
- W1925194906 cites W2127466034 @default.
- W1925194906 cites W2139444044 @default.
- W1925194906 cites W2141313743 @default.
- W1925194906 cites W2149089399 @default.
- W1925194906 cites W2149170211 @default.
- W1925194906 cites W2161579840 @default.
- W1925194906 cites W2167534889 @default.
- W1925194906 cites W2170076211 @default.
- W1925194906 cites W2467949190 @default.
- W1925194906 cites W2916719104 @default.
- W1925194906 doi "https://doi.org/10.3390/molecules200915449" @default.
- W1925194906 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6331893" @default.
- W1925194906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26343612" @default.
- W1925194906 hasPublicationYear "2015" @default.
- W1925194906 type Work @default.
- W1925194906 sameAs 1925194906 @default.
- W1925194906 citedByCount "11" @default.
- W1925194906 countsByYear W19251949062016 @default.
- W1925194906 countsByYear W19251949062017 @default.
- W1925194906 countsByYear W19251949062018 @default.
- W1925194906 countsByYear W19251949062019 @default.
- W1925194906 countsByYear W19251949062020 @default.
- W1925194906 countsByYear W19251949062021 @default.
- W1925194906 countsByYear W19251949062022 @default.
- W1925194906 countsByYear W19251949062023 @default.
- W1925194906 crossrefType "journal-article" @default.
- W1925194906 hasAuthorship W1925194906A5007797524 @default.
- W1925194906 hasAuthorship W1925194906A5016855923 @default.
- W1925194906 hasAuthorship W1925194906A5028837310 @default.
- W1925194906 hasAuthorship W1925194906A5068213552 @default.
- W1925194906 hasAuthorship W1925194906A5081645354 @default.
- W1925194906 hasAuthorship W1925194906A5086288750 @default.
- W1925194906 hasBestOaLocation W19251949061 @default.
- W1925194906 hasConcept C181199279 @default.
- W1925194906 hasConcept C185592680 @default.
- W1925194906 hasConcept C202751555 @default.
- W1925194906 hasConcept C2777752497 @default.
- W1925194906 hasConcept C2780375986 @default.
- W1925194906 hasConcept C55493867 @default.
- W1925194906 hasConceptScore W1925194906C181199279 @default.
- W1925194906 hasConceptScore W1925194906C185592680 @default.
- W1925194906 hasConceptScore W1925194906C202751555 @default.
- W1925194906 hasConceptScore W1925194906C2777752497 @default.
- W1925194906 hasConceptScore W1925194906C2780375986 @default.
- W1925194906 hasConceptScore W1925194906C55493867 @default.
- W1925194906 hasIssue "9" @default.
- W1925194906 hasLocation W19251949061 @default.
- W1925194906 hasLocation W19251949062 @default.