Matches in SemOpenAlex for { <https://semopenalex.org/work/W1925513708> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1925513708 startingPage "4182" @default.
- W1925513708 abstract "It is unknown if endotoxin reactivity in the LAL assay varies by its presentation or if this parallels differences in potency in vitro and in vivo . We compared endotoxin responses in the LAL assay, in cell cultures and in vivo when presented in different forms. Lipooligosaccharide (LOS) from Neisseria meningitidis sero B was metabolically labeled and incorporated into intact bacteria, purified membrane blebs, and aggregates ( 14 C-LOSagg). 14 C-LOS content of each was quantified as LOS-specific 14 C-3-OH fatty acids. Equivalent escalating doses of 14 C-LOS-containing bacteria, blebs, or LOSagg were tested in the LAL assay, in vivo in C3HeB/FeJ mice following intranasal instillation (monitoring induced airway inflammation), and in vitro using HEK293 cells ± CD14, MD-2, TLR4 (monitoring extracellular accumulation of IL-8). Doses of 14 C-LOS were measured by scintillation counting (our gold standard) . Potency varied depending on LOS presentation. Ranked reactivity was: in the LAL assay, blebs > LOSagg > bacteria; by airway inflammation, bacteria > blebs > LOSagg; by activation of HEK cells, LOSagg > blebs > bacteria. Differences in potency reached 10-fold for in vitro and in vivo inflammation. Across the range of doses, the ratio of LOS concentration determined by the LAL assay to the gold standard was 1.0-1.3 for blebs, 0.3-0.7 for aggregates and 0.1-0.2 for whole bacteria. How endotoxin is presented significantly affects endotoxin reactivity in the LAL, cell culture and whole animal assays. Compared to the gold standard measure, the LAL assay substantially underestimated the content of the LOS preparations for LOSagg and bacteria." @default.
- W1925513708 created "2016-06-24" @default.
- W1925513708 creator A5014457114 @default.
- W1925513708 creator A5015680363 @default.
- W1925513708 creator A5023095988 @default.
- W1925513708 creator A5038558966 @default.
- W1925513708 creator A5081784567 @default.
- W1925513708 date "2011-09-01" @default.
- W1925513708 modified "2023-09-27" @default.
- W1925513708 title "Bioactivity of 14C-labeled endotoxin in aggregates, shed blebs and whole bacteria: Potency comparison in the LAL assay, cell cultures, and mouse bioassays" @default.
- W1925513708 hasPublicationYear "2011" @default.
- W1925513708 type Work @default.
- W1925513708 sameAs 1925513708 @default.
- W1925513708 citedByCount "0" @default.
- W1925513708 crossrefType "journal-article" @default.
- W1925513708 hasAuthorship W1925513708A5014457114 @default.
- W1925513708 hasAuthorship W1925513708A5015680363 @default.
- W1925513708 hasAuthorship W1925513708A5023095988 @default.
- W1925513708 hasAuthorship W1925513708A5038558966 @default.
- W1925513708 hasAuthorship W1925513708A5081784567 @default.
- W1925513708 hasConcept C104488531 @default.
- W1925513708 hasConcept C150903083 @default.
- W1925513708 hasConcept C153911025 @default.
- W1925513708 hasConcept C185592680 @default.
- W1925513708 hasConcept C202751555 @default.
- W1925513708 hasConcept C203014093 @default.
- W1925513708 hasConcept C207001950 @default.
- W1925513708 hasConcept C2778754761 @default.
- W1925513708 hasConcept C523546767 @default.
- W1925513708 hasConcept C53227056 @default.
- W1925513708 hasConcept C54355233 @default.
- W1925513708 hasConcept C55493867 @default.
- W1925513708 hasConcept C57992300 @default.
- W1925513708 hasConcept C71924100 @default.
- W1925513708 hasConcept C86803240 @default.
- W1925513708 hasConcept C89423630 @default.
- W1925513708 hasConcept C98274493 @default.
- W1925513708 hasConceptScore W1925513708C104488531 @default.
- W1925513708 hasConceptScore W1925513708C150903083 @default.
- W1925513708 hasConceptScore W1925513708C153911025 @default.
- W1925513708 hasConceptScore W1925513708C185592680 @default.
- W1925513708 hasConceptScore W1925513708C202751555 @default.
- W1925513708 hasConceptScore W1925513708C203014093 @default.
- W1925513708 hasConceptScore W1925513708C207001950 @default.
- W1925513708 hasConceptScore W1925513708C2778754761 @default.
- W1925513708 hasConceptScore W1925513708C523546767 @default.
- W1925513708 hasConceptScore W1925513708C53227056 @default.
- W1925513708 hasConceptScore W1925513708C54355233 @default.
- W1925513708 hasConceptScore W1925513708C55493867 @default.
- W1925513708 hasConceptScore W1925513708C57992300 @default.
- W1925513708 hasConceptScore W1925513708C71924100 @default.
- W1925513708 hasConceptScore W1925513708C86803240 @default.
- W1925513708 hasConceptScore W1925513708C89423630 @default.
- W1925513708 hasConceptScore W1925513708C98274493 @default.
- W1925513708 hasLocation W19255137081 @default.
- W1925513708 hasOpenAccess W1925513708 @default.
- W1925513708 hasPrimaryLocation W19255137081 @default.
- W1925513708 hasRelatedWork W1513548069 @default.
- W1925513708 hasRelatedWork W1599444799 @default.
- W1925513708 hasRelatedWork W166421833 @default.
- W1925513708 hasRelatedWork W1999422662 @default.
- W1925513708 hasRelatedWork W2013064459 @default.
- W1925513708 hasRelatedWork W2032346259 @default.
- W1925513708 hasRelatedWork W2077109775 @default.
- W1925513708 hasRelatedWork W2087981133 @default.
- W1925513708 hasRelatedWork W2092167781 @default.
- W1925513708 hasRelatedWork W2099479741 @default.
- W1925513708 hasRelatedWork W2117091191 @default.
- W1925513708 hasRelatedWork W2123438890 @default.
- W1925513708 hasRelatedWork W2126974844 @default.
- W1925513708 hasRelatedWork W2139558307 @default.
- W1925513708 hasRelatedWork W2148757469 @default.
- W1925513708 hasRelatedWork W2319271568 @default.
- W1925513708 hasRelatedWork W2334109067 @default.
- W1925513708 hasRelatedWork W2418107714 @default.
- W1925513708 hasRelatedWork W73283374 @default.
- W1925513708 hasRelatedWork W2146768767 @default.
- W1925513708 hasVolume "38" @default.
- W1925513708 isParatext "false" @default.
- W1925513708 isRetracted "false" @default.
- W1925513708 magId "1925513708" @default.
- W1925513708 workType "article" @default.