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- W1926066365 abstract "ABSTRACT MiRNAs have been identified in various plants and animals where they function in post‐transcriptional regulation. Although studies revealed that dexamethasone play a pivotal role in the osteogenic differentiation of human bone marrow‐derived mesenchymal stem cells (hBMSCs), the identification of specific miRNAs and their regulatory roles in this process remain poorly defined. In this study, microarrays were used to analyze the miRNA expression profile of dexamethasone‐induced hBMSCs derived from three donors, and RT‐PCRs were used to confirm the microarray results. Nine upregulated miRNAs and seven downregulated miRNAs were identified. The putative target genes of these miRNAs were predicted using bioinformatics analysis. Subsequently, we focused our attention on the functional analysis of an upregulated miRNA, miR‐23a. Overexpression of miR‐23a inhibited osteogenic differentiation of hBMSCs at the cellular, mRNA, and protein levels. The results of our study provide an experimental basis for further research on miRNAs functions during osteogenic differentiation of dexamethasone‐induced hBMSCs. J. Cell. Biochem. 115: 1683–1691, 2014. © 2014 Wiley Periodicals, Inc." @default.
- W1926066365 created "2016-06-24" @default.
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- W1926066365 date "2014-08-13" @default.
- W1926066365 modified "2023-10-09" @default.
- W1926066365 title "MicroRNA Expression Profile of Dexamethasone‐Induced Human Bone Marrow‐Derived Mesenchymal Stem Cells During Osteogenic Differentiation" @default.
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- W1926066365 doi "https://doi.org/10.1002/jcb.24831" @default.
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