Matches in SemOpenAlex for { <https://semopenalex.org/work/W1926132015> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W1926132015 abstract "The interactions of the substrate analogs beta-methyl-GlcNAc, (GlcNAc)2, and (GlcNAc)3 with hen egg-white lysozyme [EC 3.2.1.17] in which an ester linkage had been formed between Glu 35 and Trp 108 (108 ester lysozyme), were studied by the circular dichroic and fluorescence techniques, and were compared with those for intact lysozyme. The binding constants of beta-methyl-GlcNAc and (GlcNAc)2 to 108 ester lysozyme were essentially the same as those for intact lysozyme in the pH range of 1 to 5. Above pH 5, the binding constants of these saccharides to 108 ester lysozyme did not change with pH, while the binding constants to intact lysozyme decreased. This indicates that Glu 35 (pK 6.0 in intact lysozyme) participates in the binding of these saccharides. The extent and direction of the pK shifts of Asp 52 (pK 3.5), Asp 48 (pK 4.4), and Asp 66 (pK 1.3) observed when beta-methyl-GlcNAc is bound to 108 ester lysozyme were the same as those for intact lysozyme. The participation of Asp 101 and Asp 66 in the binding of (GlcNAc)2 to 108 ester lysozyme was also the same as that for intact lysozyme. These findings indicate that the conformations of subsites B and C are not changed by the formation of the ester linkage. On the other hand, the binding constants of (GlcNAc)3 to 108 ester lysozyme were higher than those for intact lysozyme at all pH values studied. This result is interpreted in terms of an increase in the affinity for a GlcNAc residue of subsite D, which is situated near the esterified Glu 35." @default.
- W1926132015 created "2016-06-24" @default.
- W1926132015 creator A5032264858 @default.
- W1926132015 creator A5033053999 @default.
- W1926132015 creator A5051260077 @default.
- W1926132015 date "1976-09-01" @default.
- W1926132015 modified "2023-10-18" @default.
- W1926132015 title "Binding of Substrate Analogs to Hen Egg-white Lysozyme with an Ester Linkage between Glu 35 and Trp 1081" @default.
- W1926132015 cites W2083127366 @default.
- W1926132015 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a131296" @default.
- W1926132015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10284" @default.
- W1926132015 hasPublicationYear "1976" @default.
- W1926132015 type Work @default.
- W1926132015 sameAs 1926132015 @default.
- W1926132015 citedByCount "3" @default.
- W1926132015 crossrefType "journal-article" @default.
- W1926132015 hasAuthorship W1926132015A5032264858 @default.
- W1926132015 hasAuthorship W1926132015A5033053999 @default.
- W1926132015 hasAuthorship W1926132015A5051260077 @default.
- W1926132015 hasConcept C107824862 @default.
- W1926132015 hasConcept C181199279 @default.
- W1926132015 hasConcept C185592680 @default.
- W1926132015 hasConcept C18903297 @default.
- W1926132015 hasConcept C2776966407 @default.
- W1926132015 hasConcept C2777289219 @default.
- W1926132015 hasConcept C2778484053 @default.
- W1926132015 hasConcept C55493867 @default.
- W1926132015 hasConcept C71240020 @default.
- W1926132015 hasConcept C86803240 @default.
- W1926132015 hasConceptScore W1926132015C107824862 @default.
- W1926132015 hasConceptScore W1926132015C181199279 @default.
- W1926132015 hasConceptScore W1926132015C185592680 @default.
- W1926132015 hasConceptScore W1926132015C18903297 @default.
- W1926132015 hasConceptScore W1926132015C2776966407 @default.
- W1926132015 hasConceptScore W1926132015C2777289219 @default.
- W1926132015 hasConceptScore W1926132015C2778484053 @default.
- W1926132015 hasConceptScore W1926132015C55493867 @default.
- W1926132015 hasConceptScore W1926132015C71240020 @default.
- W1926132015 hasConceptScore W1926132015C86803240 @default.
- W1926132015 hasLocation W19261320151 @default.
- W1926132015 hasLocation W19261320152 @default.
- W1926132015 hasOpenAccess W1926132015 @default.
- W1926132015 hasPrimaryLocation W19261320151 @default.
- W1926132015 hasRelatedWork W1551116914 @default.
- W1926132015 hasRelatedWork W2037558021 @default.
- W1926132015 hasRelatedWork W2052835423 @default.
- W1926132015 hasRelatedWork W2084325434 @default.
- W1926132015 hasRelatedWork W2086520416 @default.
- W1926132015 hasRelatedWork W2235373611 @default.
- W1926132015 hasRelatedWork W2362589501 @default.
- W1926132015 hasRelatedWork W2808318619 @default.
- W1926132015 hasRelatedWork W403700794 @default.
- W1926132015 hasRelatedWork W2027424859 @default.
- W1926132015 isParatext "false" @default.
- W1926132015 isRetracted "false" @default.
- W1926132015 magId "1926132015" @default.
- W1926132015 workType "article" @default.