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• W1927076681 abstract "Since 1996, induction of competence for genetic transformation of Streptococcus pneumoniae is known to be controlled by the ComD/ComE two-component regulatory system. The mechanism of induction is generally described as involving ComD autophosphorylation, transphosphorylation of ComE and transcriptional activation by ComE∼P of the early competence (com) genes, including comX which encodes the competence-specific σX. However, none of these features has been experimentally established. Here we document the autokinase activity of ComD proteins in vitro, and provide an estimate of the stoichiometry of ComD and ComE in vivo. We report that a phosphorylmimetic mutant, ComED58E, constructed because of the failure to detect transphosphorylation of purified ComE in vitro, displays full spontaneous competence in ΔcomD cells, an that in vitro ComED58E exhibits significantly improved binding affinity for PcomCDE. We also provide evidence for a differential transcriptional activation and repression of PcomCDE and PcomX. Altogether, these data support the model of ComE∼P-dependent activation of transcription. Finally, we establish that ComE antagonizes expression of the early com genes and propose that the rapid deceleration of transcription from PcomCDE observed even in cells lacking σX is due to the progressive accumulation of ComE, which outcompetes ComE∼P." @default.
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• W1927076681 date "2012-12-10" @default.
• W1927076681 modified "2023-09-26" @default.
• W1927076681 title "ComE/ComE∼P interplay dictates activation or extinction status of pneumococcal X-state (competence)" @default.
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• W1927076681 doi "https://doi.org/10.1111/mmi.12104" @default.
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• W1927076681 hasPublicationYear "2012" @default.
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