FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1927751645> ?p ?o ?g. }

• W1927751645 endingPage "3946" @default.
• W1927751645 startingPage "3936" @default.
• W1927751645 abstract "ABSTRACTLeukocyte adhesion to the extracellular matrix (ECM) is tightly controlled and is vital for the immune response. Circulating lymphocytes leave the bloodstream and adhere to ECM components at sites of inflammation and lymphoid tissues. Mechanisms for regulating T-lymphocyte–ECM adhesion include (i) an alteration in the affinity of cell surface integrin receptors for their extracellular ligands and (ii) an alteration of events following postreceptor occupancy (e.g., cell spreading). Whereas H-Ras and R-Ras were previously shown to affect T-cell adhesion by altering the affinity state of the integrin receptors, no signaling molecule has been identified for the second mechanism. In this study, we demonstrated that expression of an activated mutant of Rac triggered dramatic spreading of T cells and their increased adhesion on immobilized fibronectin in an integrin-dependent manner. This effect was not mimicked by expression of activated mutant forms of Rho, Cdc42, H-Ras, or ARF6, indicating the unique role of Rac in this event. The Rac-induced spreading was accompanied by specific cytoskeletal rearrangements. Also, a clustering of integrins at sites of cell adhesion and at the peripheral edges of spread cells was observed. We demonstrate that expression of RacV12 did not alter the level of expression of cell surface integrins or the affinity state of the integrin receptors. Moreover, our results indicate that Rac plays a role in the regulation of T-cell adhesion by a mechanism involving cell spreading, rather than by altering the level of expression or the affinity of the integrin receptors. Furthermore, we show that the Rac-mediated signaling pathway leading to spreading of T lymphocytes did not require activation of c-Jun kinase, serum response factor, or pp70S6 kinase but appeared to involve a phospholipid kinase. ACKNOWLEDGMENTSWe thank P. D. Stahl for generous support; E. J. Brown, J. Schorey, and H. Shenoi for reagents, helpful discussions, and critical reading of the manuscript; A. Iafrate and J. Skowronski for cells, protocols, and advice for T-cell electroporation; R. Packer and M. Coronesi for technical assistance; and N. Novach and T. A. Yednock for providing antibodies.This work was supported by grants of the National Cancer Institute (1R01CA 72982-OIAI) and an award from the Sidney Kimmel Foundation for Cancer Research and The V Foundation to L.V.A. and by grants from the Leukemia Research Foundation and the American Cancer Society (ACS-IRG 36-39) to C.D.-S. C.D.-S. is a Leukemia Society of America Special Fellow." @default.
• W1927751645 created "2016-06-24" @default.
• W1927751645 creator A5021978606 @default.
• W1927751645 creator A5029768703 @default.
• W1927751645 creator A5072827834 @default.
• W1927751645 date "1998-07-01" @default.
• W1927751645 modified "2023-10-10" @default.
• W1927751645 title "Rac Regulates Integrin-Mediated Spreading and Increased Adhesion of T Lymphocytes" @default.
• W1927751645 cites W1512678034 @default.
• W1927751645 cites W1606615500 @default.
• W1927751645 cites W1968647003 @default.
• W1927751645 cites W1968690892 @default.
• W1927751645 cites W1969560454 @default.
• W1927751645 cites W1972287742 @default.
• W1927751645 cites W1972317299 @default.
• W1927751645 cites W1977701732 @default.
• W1927751645 cites W1979797395 @default.
• W1927751645 cites W1980830316 @default.
• W1927751645 cites W1980952363 @default.
• W1927751645 cites W1987568997 @default.
• W1927751645 cites W1987888289 @default.
• W1927751645 cites W1994811935 @default.
• W1927751645 cites W1998749044 @default.
• W1927751645 cites W2002410732 @default.
• W1927751645 cites W2003324016 @default.
• W1927751645 cites W2007660746 @default.
• W1927751645 cites W2013867315 @default.
• W1927751645 cites W2016384716 @default.
• W1927751645 cites W2020006960 @default.
• W1927751645 cites W2027810531 @default.
• W1927751645 cites W2029231027 @default.
• W1927751645 cites W2034070845 @default.
• W1927751645 cites W2039296434 @default.
• W1927751645 cites W2040283218 @default.
• W1927751645 cites W2042626190 @default.
• W1927751645 cites W2047605827 @default.
• W1927751645 cites W2056274982 @default.
• W1927751645 cites W2062582674 @default.
• W1927751645 cites W2071933656 @default.
• W1927751645 cites W2074937633 @default.
• W1927751645 cites W2075887159 @default.
• W1927751645 cites W2077792958 @default.
• W1927751645 cites W2079892535 @default.
• W1927751645 cites W2092583350 @default.
• W1927751645 cites W2093226717 @default.
• W1927751645 cites W2095141538 @default.
• W1927751645 cites W2095430146 @default.
• W1927751645 cites W2096791952 @default.
• W1927751645 cites W2099731590 @default.
• W1927751645 cites W2099942500 @default.
• W1927751645 cites W2101480539 @default.
• W1927751645 cites W2104045440 @default.
• W1927751645 cites W2106200811 @default.
• W1927751645 cites W2111604219 @default.
• W1927751645 cites W2119741323 @default.
• W1927751645 cites W2122360544 @default.
• W1927751645 cites W2134073082 @default.
• W1927751645 cites W2135121318 @default.
• W1927751645 cites W2150293212 @default.
• W1927751645 cites W2164939655 @default.
• W1927751645 cites W2169019804 @default.
• W1927751645 cites W2170906235 @default.
• W1927751645 cites W2171303172 @default.
• W1927751645 cites W2231016210 @default.
• W1927751645 cites W4235285967 @default.
• W1927751645 doi "https://doi.org/10.1128/mcb.18.7.3936" @default.
• W1927751645 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/108978" @default.
• W1927751645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9632778" @default.
• W1927751645 hasPublicationYear "1998" @default.
• W1927751645 type Work @default.
• W1927751645 sameAs 1927751645 @default.
• W1927751645 citedByCount "152" @default.
• W1927751645 countsByYear W19277516452012 @default.
• W1927751645 countsByYear W19277516452013 @default.
• W1927751645 countsByYear W19277516452014 @default.
• W1927751645 countsByYear W19277516452016 @default.
• W1927751645 countsByYear W19277516452017 @default.
• W1927751645 countsByYear W19277516452019 @default.
• W1927751645 countsByYear W19277516452020 @default.
• W1927751645 countsByYear W19277516452022 @default.
• W1927751645 countsByYear W19277516452023 @default.
• W1927751645 crossrefType "journal-article" @default.
• W1927751645 hasAuthorship W1927751645A5021978606 @default.
• W1927751645 hasAuthorship W1927751645A5029768703 @default.
• W1927751645 hasAuthorship W1927751645A5072827834 @default.
• W1927751645 hasBestOaLocation W19277516451 @default.
• W1927751645 hasConcept C1491633281 @default.
• W1927751645 hasConcept C16224149 @default.
• W1927751645 hasConcept C167454224 @default.
• W1927751645 hasConcept C170493617 @default.
• W1927751645 hasConcept C178790620 @default.
• W1927751645 hasConcept C185592680 @default.
• W1927751645 hasConcept C189165786 @default.
• W1927751645 hasConcept C195687474 @default.
• W1927751645 hasConcept C55493867 @default.
• W1927751645 hasConcept C84416704 @default.
• W1927751645 hasConcept C85789140 @default.
• W1927751645 hasConcept C86492073 @default.

• next