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- W192812846 abstract "Of the variety of molecular weight methods applicable to DNA, the sedimentation and/or viscosity method, and the electron microscopic-length method have proved to be the most satisfactory and the most widely accepted. These two techniques are additionally useful because of the information about conformation and topology which they can provide. However, in view of the pitfalls of sedimentation-viscosity measurements and the empirical nature of their relation to molecular weight, and because of the presence of an unknown source of variability in the electron microscopic method,109 absolute molecular weights with less than 10% uncertainty cannot be claimed, and errors as large as 20% are probably not uncommon, as inspection of Table I will suggest. The situation today is nevertheless infinitely improved over what it was a few years ago when errors by factors of two and more were common-place in reported molecular weights of DNA. In addition, there now exist several other independent techniques which provide molecular weight results consistent with those obtained by the two popular methods. Most importantly, “standard” DNA's are now available from the dozen DNA-containing viruses listed in Table I. With further study the molecular weights of the nucleic acids of these and other DNA viruses will be more accurately established. The methodology of DNA molecular weight determinations therefore appears to be capable of meeting the demands put upon it by current experimentation with intact viral chromosomes. Eventually the molecular weights of bacterial chromosomes may also be routinely determined, a possibility suggested by the last three entries in Table I." @default.
- W192812846 created "2016-06-24" @default.
- W192812846 creator A5089898273 @default.
- W192812846 date "1968-01-01" @default.
- W192812846 modified "2023-10-17" @default.
- W192812846 title "[128] Molecular weight and conformation of DNA" @default.
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- W192812846 doi "https://doi.org/10.1016/0076-6879(67)12153-8" @default.
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