FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1928181584> ?p ?o ?g. }

• W1928181584 endingPage "6065" @default.
• W1928181584 startingPage "6056" @default.
• W1928181584 abstract "ABSTRACT Following receptor binding and internalization, intracellular trafficking of adenovirus (Ad) among subgroups B and C is different, with significant amounts of Ad serotype 7 (Ad7) (subgroup B) virions found in cytoplasm during the initial hours of infection while Ad5 (subgroup C) virions rapidly translocate to the nucleus. To evaluate the role of the fiber in these differences, we examined intracellular trafficking of Ad5, Ad7, and Ad5f7 (a chimeric vector composed of the Ad5 capsid with the fiber replaced by the Ad7 fiber) by conjugating Ad capsids directly with Cy3 fluorescent dye, permitting the trafficking of the capsids to be examined by fluorescence microscopy. The human lung carcinoma cell line A549 was infected with Cy3-conjugated viruses for 10 min followed by a 1-h incubation. Ad5 virions rapidly translocated to the nucleus (within 1 h of infection), while Ad7 virions were widely distributed in the cytoplasm at the same time point. Interestingly, chimeric Ad5f7 virions behaved similarly to Ad7 but not Ad5. In this regard, the percentages of nuclear localization of Ad5, Ad7, and Ad5f7 at 1 h following infection were 72% ± 4%, 32% ± 6%, and 38% ± 2%, respectively. Consistent with these observations, fluorescence in situ hybridization demonstrated that most of the Ad5 DNA was detected at the nucleus after 1 h, but at the same time point, DNA of Ad7 and Ad5f7 was distributed in both the nucleus and cytoplasm. Quantification of the kinetics of Ad genomic DNA delivery to the nucleus using a fluorogenic probe-based PCR assay (TaqMan PCR) demonstrated that the percentages of nuclear association of Ad5 DNA and Ad5f7 DNA at 1 h postinfection were 80% ± 13% and 43% ± 1%, respectively. Although it has been generally accepted that Ad fiber protein mediates attachment of virions to cells and that fibers dissociate during endocytic uptake, these data suggest that in addition to mediating binding to the cell surface, fiber likely modulates intracellular trafficking as well." @default.
• W1928181584 created "2016-06-24" @default.
• W1928181584 creator A5001983513 @default.
• W1928181584 creator A5020151133 @default.
• W1928181584 creator A5021806533 @default.
• W1928181584 creator A5027155026 @default.
• W1928181584 creator A5050729392 @default.
• W1928181584 creator A5064433832 @default.
• W1928181584 creator A5085050178 @default.
• W1928181584 date "1999-07-01" @default.
• W1928181584 modified "2023-09-27" @default.
• W1928181584 title "Fiber Swap between Adenovirus Subgroups B and C Alters Intracellular Trafficking of Adenovirus Gene Transfer Vectors" @default.
• W1928181584 cites W1486052360 @default.
• W1928181584 cites W1489938529 @default.
• W1928181584 cites W1506470251 @default.
• W1928181584 cites W1525067447 @default.
• W1928181584 cites W1525926169 @default.
• W1928181584 cites W1528537522 @default.
• W1928181584 cites W1549192595 @default.
• W1928181584 cites W1571169370 @default.
• W1928181584 cites W1576644054 @default.
• W1928181584 cites W1740655975 @default.
• W1928181584 cites W18142307 @default.
• W1928181584 cites W1857650329 @default.
• W1928181584 cites W1858472669 @default.
• W1928181584 cites W1913756728 @default.
• W1928181584 cites W1938253145 @default.
• W1928181584 cites W1977752983 @default.
• W1928181584 cites W1980401440 @default.
• W1928181584 cites W1983682307 @default.
• W1928181584 cites W1991710882 @default.
• W1928181584 cites W1992677824 @default.
• W1928181584 cites W1998128870 @default.
• W1928181584 cites W2006585409 @default.
• W1928181584 cites W2009207470 @default.
• W1928181584 cites W2027980197 @default.
• W1928181584 cites W203852859 @default.
• W1928181584 cites W2051758487 @default.
• W1928181584 cites W2065392104 @default.
• W1928181584 cites W2077541686 @default.
• W1928181584 cites W2079864692 @default.
• W1928181584 cites W2084263686 @default.
• W1928181584 cites W2086172854 @default.
• W1928181584 cites W2090781939 @default.
• W1928181584 cites W2098264302 @default.
• W1928181584 cites W2111871633 @default.
• W1928181584 cites W2112836649 @default.
• W1928181584 cites W2116071714 @default.
• W1928181584 cites W2120138824 @default.
• W1928181584 cites W2123745267 @default.
• W1928181584 cites W2125923808 @default.
• W1928181584 cites W2129333552 @default.
• W1928181584 cites W2129859001 @default.
• W1928181584 cites W2130451879 @default.
• W1928181584 cites W2133388585 @default.
• W1928181584 cites W2133822846 @default.
• W1928181584 cites W2134430211 @default.
• W1928181584 cites W2134504308 @default.
• W1928181584 cites W2137408987 @default.
• W1928181584 cites W2158071204 @default.
• W1928181584 cites W2159913430 @default.
• W1928181584 cites W2170250011 @default.
• W1928181584 cites W2313269464 @default.
• W1928181584 cites W4231136244 @default.
• W1928181584 doi "https://doi.org/10.1128/jvi.73.7.6056-6065.1999" @default.
• W1928181584 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/112667" @default.
• W1928181584 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10364358" @default.
• W1928181584 hasPublicationYear "1999" @default.
• W1928181584 type Work @default.
• W1928181584 sameAs 1928181584 @default.
• W1928181584 citedByCount "147" @default.
• W1928181584 countsByYear W19281815842012 @default.
• W1928181584 countsByYear W19281815842013 @default.
• W1928181584 countsByYear W19281815842014 @default.
• W1928181584 countsByYear W19281815842015 @default.
• W1928181584 countsByYear W19281815842016 @default.
• W1928181584 countsByYear W19281815842017 @default.
• W1928181584 countsByYear W19281815842018 @default.
• W1928181584 countsByYear W19281815842019 @default.
• W1928181584 countsByYear W19281815842020 @default.
• W1928181584 countsByYear W19281815842021 @default.
• W1928181584 crossrefType "journal-article" @default.
• W1928181584 hasAuthorship W1928181584A5001983513 @default.
• W1928181584 hasAuthorship W1928181584A5020151133 @default.
• W1928181584 hasAuthorship W1928181584A5021806533 @default.
• W1928181584 hasAuthorship W1928181584A5027155026 @default.
• W1928181584 hasAuthorship W1928181584A5050729392 @default.
• W1928181584 hasAuthorship W1928181584A5064433832 @default.
• W1928181584 hasAuthorship W1928181584A5085050178 @default.
• W1928181584 hasBestOaLocation W19281815841 @default.
• W1928181584 hasConcept C104317684 @default.
• W1928181584 hasConcept C139770010 @default.
• W1928181584 hasConcept C1491633281 @default.
• W1928181584 hasConcept C153911025 @default.
• W1928181584 hasConcept C159047783 @default.
• W1928181584 hasConcept C190062978 @default.
• W1928181584 hasConcept C202878990 @default.
• W1928181584 hasConcept C2522874641 @default.

• next