Matches in SemOpenAlex for { <https://semopenalex.org/work/W1930010684> ?p ?o ?g. }
- W1930010684 endingPage "326" @default.
- W1930010684 startingPage "317" @default.
- W1930010684 abstract "To drive neural differentiation of mouse embryonic stem (ES) cells, various culture protocols have been previously developed that all require the formation of embryoid bodies, usually combined with a treatment by all-trans retinoic acid (aRA). Here, we investigated whether or not neural differentiation can also occur in a simplified monolayer culture. Mouse ES cells were plated in serum-containing DMEM media with and without aRA and grown under these conditions for 2 days. Then, the cells were transferred to fresh serum-containing DMEM media and/or to serum-free DMEM/F12 media supplemented with a mixture of insulin, transferrin, selenium, and fibronectin (ITSF) for further culture. The changes in cell morphology and in the expression of selected molecular markers were monitored. Generally, in contrast to all the others, the protocol consisting of a 2-day culture in serum-containing DMEM followed by continuous exposure to the ITSF supplement in DMEM/F12 drove a vast majority of ES cells to generate phenotypic signs of neural lineage. Altogether, neural differentiation can be achieved in vitro without the step involving the formation of embryoid bodies as well as the treatment by aRA." @default.
- W1930010684 created "2016-06-24" @default.
- W1930010684 creator A5016395037 @default.
- W1930010684 creator A5033872292 @default.
- W1930010684 creator A5035172974 @default.
- W1930010684 creator A5059929737 @default.
- W1930010684 creator A5087317439 @default.
- W1930010684 date "2002-07-01" @default.
- W1930010684 modified "2023-10-14" @default.
- W1930010684 title "Neural differentiation of mouse embryonic stem cells grown in monolayer" @default.
- W1930010684 cites W1936342032 @default.
- W1930010684 cites W1948275303 @default.
- W1930010684 cites W1967719396 @default.
- W1930010684 cites W1998225973 @default.
- W1930010684 cites W2001859961 @default.
- W1930010684 cites W2021494076 @default.
- W1930010684 cites W2024886013 @default.
- W1930010684 cites W2027246053 @default.
- W1930010684 cites W2043852393 @default.
- W1930010684 cites W2044918998 @default.
- W1930010684 cites W2045899482 @default.
- W1930010684 cites W2057159461 @default.
- W1930010684 cites W2064496329 @default.
- W1930010684 cites W2064919591 @default.
- W1930010684 cites W2069826560 @default.
- W1930010684 cites W2072198497 @default.
- W1930010684 cites W2080459068 @default.
- W1930010684 cites W2088162801 @default.
- W1930010684 cites W2089252344 @default.
- W1930010684 cites W2104244471 @default.
- W1930010684 cites W2112835392 @default.
- W1930010684 cites W2135668873 @default.
- W1930010684 cites W2161687343 @default.
- W1930010684 cites W2408161557 @default.
- W1930010684 cites W2417171523 @default.
- W1930010684 doi "https://doi.org/10.1051/rnd:2002028" @default.
- W1930010684 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12510873" @default.
- W1930010684 hasPublicationYear "2002" @default.
- W1930010684 type Work @default.
- W1930010684 sameAs 1930010684 @default.
- W1930010684 citedByCount "39" @default.
- W1930010684 countsByYear W19300106842012 @default.
- W1930010684 countsByYear W19300106842013 @default.
- W1930010684 countsByYear W19300106842014 @default.
- W1930010684 countsByYear W19300106842015 @default.
- W1930010684 countsByYear W19300106842016 @default.
- W1930010684 countsByYear W19300106842017 @default.
- W1930010684 countsByYear W19300106842020 @default.
- W1930010684 countsByYear W19300106842021 @default.
- W1930010684 crossrefType "journal-article" @default.
- W1930010684 hasAuthorship W1930010684A5016395037 @default.
- W1930010684 hasAuthorship W1930010684A5033872292 @default.
- W1930010684 hasAuthorship W1930010684A5035172974 @default.
- W1930010684 hasAuthorship W1930010684A5059929737 @default.
- W1930010684 hasAuthorship W1930010684A5087317439 @default.
- W1930010684 hasBestOaLocation W19300106841 @default.
- W1930010684 hasConcept C104317684 @default.
- W1930010684 hasConcept C136834591 @default.
- W1930010684 hasConcept C143948831 @default.
- W1930010684 hasConcept C145103041 @default.
- W1930010684 hasConcept C148738053 @default.
- W1930010684 hasConcept C153911025 @default.
- W1930010684 hasConcept C16685009 @default.
- W1930010684 hasConcept C20875687 @default.
- W1930010684 hasConcept C2781121885 @default.
- W1930010684 hasConcept C28328180 @default.
- W1930010684 hasConcept C54355233 @default.
- W1930010684 hasConcept C55493867 @default.
- W1930010684 hasConcept C71924100 @default.
- W1930010684 hasConcept C81885089 @default.
- W1930010684 hasConcept C86803240 @default.
- W1930010684 hasConcept C95444343 @default.
- W1930010684 hasConceptScore W1930010684C104317684 @default.
- W1930010684 hasConceptScore W1930010684C136834591 @default.
- W1930010684 hasConceptScore W1930010684C143948831 @default.
- W1930010684 hasConceptScore W1930010684C145103041 @default.
- W1930010684 hasConceptScore W1930010684C148738053 @default.
- W1930010684 hasConceptScore W1930010684C153911025 @default.
- W1930010684 hasConceptScore W1930010684C16685009 @default.
- W1930010684 hasConceptScore W1930010684C20875687 @default.
- W1930010684 hasConceptScore W1930010684C2781121885 @default.
- W1930010684 hasConceptScore W1930010684C28328180 @default.
- W1930010684 hasConceptScore W1930010684C54355233 @default.
- W1930010684 hasConceptScore W1930010684C55493867 @default.
- W1930010684 hasConceptScore W1930010684C71924100 @default.
- W1930010684 hasConceptScore W1930010684C81885089 @default.
- W1930010684 hasConceptScore W1930010684C86803240 @default.
- W1930010684 hasConceptScore W1930010684C95444343 @default.
- W1930010684 hasIssue "4" @default.
- W1930010684 hasLocation W19300106841 @default.
- W1930010684 hasLocation W19300106842 @default.
- W1930010684 hasLocation W19300106843 @default.
- W1930010684 hasOpenAccess W1930010684 @default.
- W1930010684 hasPrimaryLocation W19300106841 @default.
- W1930010684 hasRelatedWork W145559274 @default.
- W1930010684 hasRelatedWork W166309462 @default.
- W1930010684 hasRelatedWork W18302978 @default.
- W1930010684 hasRelatedWork W2029915148 @default.