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- W193011012 abstract "Myxococcus xanthus is a non pathogenic gram negative bacteria that secretes numerous proteins into its growth medium. The total amount of extra cellular proteins increases up to two fold with various conditions of culture : temperature, shaking, medium, ions Ca2+. Myxococcus Xanthus is also able to secrete foreign proteins. These proteins are coded by genes that are cloned into the plasmid RP4. This plasmid is used as a vector and is maintained in Myxococcus Xanthus integrated into the chromosome. 1- The polygalacturonate lyases from Erwinia chrysanthem are constituvely produced, but the activity measured in the culture supernatant is very low because these enzymes are very sensitive to the proteases secreted by Myxococcus Xanthus. 2- The pH 2,5 acidic phosphatise from Escherichia coli is first produced mainly in the periplasm, then at the end of log phase it is recovered from the extra cellular fluid. Growth conditions were screened in order to optimize acidic phosphatise production. We have found conditions that significantly increase the total amount of proteins in the medium, and trigger even more the production of acidic phosphatise (up to 3 fold). When cells are immobilized in 2% carraghenan beads, production of total proteins and acidic phosphatase outside the cells were unmodified, whereas the proteins and activity are differently distributed between the gel and the liquid medium : most extra cellular proteins are trapped within the gel (75%) while acidic phosphatise activity is mostly recovered in the liquid medium. However extra cellular proteolytic and bacteriolytic activities produced into the medium or inside the beads are greatly increased and stabilized as compared to the control. Also immobilized cells grown in a reactor secreted continuously native and foreign proteins." @default.
- W193011012 created "2016-06-24" @default.
- W193011012 creator A5033103967 @default.
- W193011012 date "1986-01-01" @default.
- W193011012 modified "2023-09-26" @default.
- W193011012 title "Effet des conditions de croissance sur la production de protéines endogènes ou étrangères par Myxococcus xanthus" @default.
- W193011012 hasPublicationYear "1986" @default.
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