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- W1930436706 abstract "In Helicobacter pylori, iron balance is controlled by the Ferric uptake regulator (Fur), an iron-sensing repressor protein that typically regulates expression of genes implicated in iron transport and storage. Herein, we carried out extensive analysis of Fur-regulated promoters and identified a 7-1-7 motif with dyad symmetry (5′-TAATAATnATTATTA-3′), which functions as the Fur box core sequence of H. pylori. Addition of this sequence to the promoter region of a typically non-Fur regulated gene was sufficient to impose Fur-dependent regulation in vivo. Moreover, mutation of this sequence within Fur-controlled promoters negated regulation. Analysis of the H. pylori chromosome for the occurrence of the Fur box established the existence of well-conserved Fur boxes in the promoters of numerous known Fur-regulated genes, and revealed novel putative Fur targets. Transcriptional analysis of the new candidate genes demonstrated Fur-dependent repression of HPG27_51, HPG27_52, HPG27_199, HPG27_445, HPG27_825 and HPG27_1063, as well as Fur-mediated activation of the cytotoxin associated gene A, cagA (HPG27_507). Furthermore, electrophoretic mobility shift assays confirmed specific binding of Fur to the promoters of each of these genes. Future experiments will determine whether loss of Fur regulation of any of these particular genes contributes to the defects in colonization exhibited by the H. pylori fur mutant." @default.
- W1930436706 created "2016-06-24" @default.
- W1930436706 creator A5001544917 @default.
- W1930436706 creator A5008876152 @default.
- W1930436706 creator A5020976270 @default.
- W1930436706 creator A5078961974 @default.
- W1930436706 date "2012-05-14" @default.
- W1930436706 modified "2023-09-25" @default.
- W1930436706 title "Detailed analysis of Helicobacter pylori Fur-regulated promoters reveals a Fur box core sequence and novel Fur-regulated genes" @default.
- W1930436706 cites W1517316728 @default.
- W1930436706 cites W1567229835 @default.
- W1930436706 cites W1588482868 @default.
- W1930436706 cites W1594033851 @default.
- W1930436706 cites W1685828115 @default.
- W1930436706 cites W1713173235 @default.
- W1930436706 cites W1763272466 @default.
- W1930436706 cites W1811620130 @default.
- W1930436706 cites W1812000907 @default.
- W1930436706 cites W1931298889 @default.
- W1930436706 cites W1962539160 @default.
- W1930436706 cites W1969026716 @default.
- W1930436706 cites W1970053569 @default.
- W1930436706 cites W1971849405 @default.
- W1930436706 cites W1994193521 @default.
- W1930436706 cites W1994852088 @default.
- W1930436706 cites W2005537589 @default.
- W1930436706 cites W2007710769 @default.
- W1930436706 cites W2024486633 @default.
- W1930436706 cites W2029992296 @default.
- W1930436706 cites W2032638698 @default.
- W1930436706 cites W2039095577 @default.
- W1930436706 cites W2044320282 @default.
- W1930436706 cites W2049844283 @default.
- W1930436706 cites W2054735322 @default.
- W1930436706 cites W2062896692 @default.
- W1930436706 cites W2071735418 @default.
- W1930436706 cites W2072779885 @default.
- W1930436706 cites W2072868251 @default.
- W1930436706 cites W2079054594 @default.
- W1930436706 cites W2082410833 @default.
- W1930436706 cites W2084701457 @default.
- W1930436706 cites W2091019963 @default.
- W1930436706 cites W2093196686 @default.
- W1930436706 cites W2097099773 @default.
- W1930436706 cites W2098798603 @default.
- W1930436706 cites W2100223450 @default.
- W1930436706 cites W2102713587 @default.
- W1930436706 cites W2107710361 @default.
- W1930436706 cites W2109275139 @default.
- W1930436706 cites W2110132014 @default.
- W1930436706 cites W2110830984 @default.
- W1930436706 cites W2111651784 @default.
- W1930436706 cites W2114728811 @default.
- W1930436706 cites W2115567113 @default.
- W1930436706 cites W2120143757 @default.
- W1930436706 cites W2121871451 @default.
- W1930436706 cites W2122839142 @default.
- W1930436706 cites W2127818778 @default.
- W1930436706 cites W2131282911 @default.
- W1930436706 cites W2131884256 @default.
- W1930436706 cites W2132417094 @default.
- W1930436706 cites W2133923782 @default.
- W1930436706 cites W2136657870 @default.
- W1930436706 cites W2137015675 @default.
- W1930436706 cites W2138406202 @default.
- W1930436706 cites W2138898436 @default.
- W1930436706 cites W2138961208 @default.
- W1930436706 cites W2139704756 @default.
- W1930436706 cites W2142316694 @default.
- W1930436706 cites W2143670813 @default.
- W1930436706 cites W2148922163 @default.
- W1930436706 cites W2149644705 @default.
- W1930436706 cites W2151395223 @default.
- W1930436706 cites W2152882631 @default.
- W1930436706 cites W2155544436 @default.
- W1930436706 cites W2157826403 @default.
- W1930436706 cites W2157917726 @default.
- W1930436706 cites W2158266834 @default.
- W1930436706 cites W2159352528 @default.
- W1930436706 cites W2159656294 @default.
- W1930436706 cites W2162338734 @default.
- W1930436706 cites W2164698153 @default.
- W1930436706 cites W2164927343 @default.
- W1930436706 cites W2165913728 @default.
- W1930436706 cites W2170212324 @default.
- W1930436706 cites W2170700562 @default.
- W1930436706 cites W2171740941 @default.
- W1930436706 cites W2172177565 @default.
- W1930436706 cites W4211041279 @default.
- W1930436706 cites W4254070241 @default.
- W1930436706 doi "https://doi.org/10.1111/j.1365-2958.2012.08066.x" @default.
- W1930436706 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3359417" @default.
- W1930436706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22507395" @default.
- W1930436706 hasPublicationYear "2012" @default.
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