Matches in SemOpenAlex for { <https://semopenalex.org/work/W1930600971> ?p ?o ?g. }
- W1930600971 endingPage "e0136533" @default.
- W1930600971 startingPage "e0136533" @default.
- W1930600971 abstract "Pseudomonas aeruginosa employs a type three secretion system to facilitate infections in mammalian hosts. The operons encoding genes of structural components of the secretion machinery and associated virulence factors are all under the control of the AraC-type transcriptional activator protein, ExsA. ExsA belongs to a unique subfamily of AraC-proteins that is regulated through protein-protein contacts rather than small molecule ligands. Prior to infection, ExsA is inhibited through a direct interaction with the anti-activator ExsD. To activate ExsA upon host cell contact this interaction is disrupted by the anti-antiactivator protein ExsC. Here we report the crystal structure of the regulatory domain of ExsA, which is known to mediate ExsA dimerization as well as ExsD binding. The crystal structure suggests two models for the ExsA dimer. Both models confirmed the previously shown involvement of helix α-3 in ExsA dimerization but one also suggest a role for helix α-2. These structural data are supported by the observation that a mutation in α-2 greatly diminished the ability of ExsA to activate transcription in vitro. Additional in vitro transcription studies revealed that a conserved pocket, used by AraC and the related ToxT protein for the binding of small molecule regulators, although present in ExsA is not involved in binding of ExsD." @default.
- W1930600971 created "2016-06-24" @default.
- W1930600971 creator A5009970291 @default.
- W1930600971 creator A5014365904 @default.
- W1930600971 creator A5060572750 @default.
- W1930600971 creator A5078223433 @default.
- W1930600971 date "2015-08-28" @default.
- W1930600971 modified "2023-09-30" @default.
- W1930600971 title "Structural Analysis of the Regulatory Domain of ExsA, a Key Transcriptional Regulator of the Type Three Secretion System in Pseudomonas aeruginosa" @default.
- W1930600971 cites W1506811553 @default.
- W1930600971 cites W1603154340 @default.
- W1930600971 cites W1689368762 @default.
- W1930600971 cites W1838561722 @default.
- W1930600971 cites W1964112944 @default.
- W1930600971 cites W1964284806 @default.
- W1930600971 cites W1966642247 @default.
- W1930600971 cites W1971247694 @default.
- W1930600971 cites W1976754726 @default.
- W1930600971 cites W1986860360 @default.
- W1930600971 cites W1986889683 @default.
- W1930600971 cites W1987706049 @default.
- W1930600971 cites W1991774743 @default.
- W1930600971 cites W1996214149 @default.
- W1930600971 cites W2000228343 @default.
- W1930600971 cites W2001366460 @default.
- W1930600971 cites W2001701005 @default.
- W1930600971 cites W2003849191 @default.
- W1930600971 cites W2004677549 @default.
- W1930600971 cites W2005229359 @default.
- W1930600971 cites W2007747684 @default.
- W1930600971 cites W2011065894 @default.
- W1930600971 cites W2018391128 @default.
- W1930600971 cites W2019152307 @default.
- W1930600971 cites W2020145340 @default.
- W1930600971 cites W2020755151 @default.
- W1930600971 cites W2022260094 @default.
- W1930600971 cites W2026352553 @default.
- W1930600971 cites W2026767056 @default.
- W1930600971 cites W2027139943 @default.
- W1930600971 cites W2044104169 @default.
- W1930600971 cites W2050294818 @default.
- W1930600971 cites W2050588950 @default.
- W1930600971 cites W2053024480 @default.
- W1930600971 cites W2053450384 @default.
- W1930600971 cites W2058246783 @default.
- W1930600971 cites W2074044150 @default.
- W1930600971 cites W2079653040 @default.
- W1930600971 cites W2084046561 @default.
- W1930600971 cites W2084310228 @default.
- W1930600971 cites W2096337071 @default.
- W1930600971 cites W2097212385 @default.
- W1930600971 cites W2099804686 @default.
- W1930600971 cites W2106709210 @default.
- W1930600971 cites W2108959691 @default.
- W1930600971 cites W2110120447 @default.
- W1930600971 cites W2110553890 @default.
- W1930600971 cites W2110918397 @default.
- W1930600971 cites W2114735044 @default.
- W1930600971 cites W2116921113 @default.
- W1930600971 cites W2123047270 @default.
- W1930600971 cites W2123125113 @default.
- W1930600971 cites W2124026197 @default.
- W1930600971 cites W2125371049 @default.
- W1930600971 cites W2126579640 @default.
- W1930600971 cites W2128503331 @default.
- W1930600971 cites W2131731445 @default.
- W1930600971 cites W2131795398 @default.
- W1930600971 cites W2132782746 @default.
- W1930600971 cites W2135554057 @default.
- W1930600971 cites W2137057501 @default.
- W1930600971 cites W2140167462 @default.
- W1930600971 cites W2142600143 @default.
- W1930600971 cites W2144264917 @default.
- W1930600971 cites W2146143815 @default.
- W1930600971 cites W2146606085 @default.
- W1930600971 cites W2148523443 @default.
- W1930600971 cites W2154197653 @default.
- W1930600971 cites W2154281186 @default.
- W1930600971 cites W2154923841 @default.
- W1930600971 cites W2155184228 @default.
- W1930600971 cites W2155228106 @default.
- W1930600971 cites W2156521865 @default.
- W1930600971 cites W2156832389 @default.
- W1930600971 cites W2160898059 @default.
- W1930600971 cites W2161014327 @default.
- W1930600971 cites W2163341755 @default.
- W1930600971 cites W2166826452 @default.
- W1930600971 cites W2166884322 @default.
- W1930600971 cites W2167106460 @default.
- W1930600971 cites W2171961301 @default.
- W1930600971 cites W2180229411 @default.
- W1930600971 cites W2321428848 @default.
- W1930600971 cites W4240745890 @default.
- W1930600971 doi "https://doi.org/10.1371/journal.pone.0136533" @default.
- W1930600971 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4552939" @default.
- W1930600971 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26317977" @default.
- W1930600971 hasPublicationYear "2015" @default.
- W1930600971 type Work @default.