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- W1930603914 abstract "A putative maltogenic amylase gene (DGMA) was cloned from the Deinococcus geothermalis DSM 11300 genome using the polymerase chain reaction. The gene encoded 608 amino acids with a predicted molecular mass of 68,704 Da. The recombinant DGMA was constitutively expressed using the pHCXHD plasmid. As expected, the recombinant DGMA hydrolyzed cyclodextrins and starch to maltose and pullulan to panose by cleaving the α-(1,4)- glycosidic linkages, as observed for typical maltogenic amylases. Characterization of the recombinant DGMA revealed that the highest maltogenic amylase activity occurred at 40 ℃ and pH 6.0. The half-life of catalytic activity at 65 ℃ and 55 ℃ were 8.2 min and 187.4 min, respectively. DGMA mainly hydrolyzed β-cyclodextrin, soluble starch, and pullulan and its efficient ratio of those substrates was 9:4.5:1." @default.
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- W1930603914 date "2011-06-01" @default.
- W1930603914 modified "2023-09-24" @default.
- W1930603914 title "Deinococcus geothermalis 유래 maltogenic amylase의 유전자 발현 및 특성확인" @default.
- W1930603914 hasPublicationYear "2011" @default.
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