Matches in SemOpenAlex for { <https://semopenalex.org/work/W1930968> ?p ?o ?g. }
- W1930968 endingPage "S97" @default.
- W1930968 startingPage "S92" @default.
- W1930968 abstract "Acetylcholinesterase (AChE; EC3.1.1.7) is well known for its role in the hydrolysis of acetylcholine at cholinergic synapses to terminate neurotransmission. In addition to its synaptic presence, AChE has been found to be in non-cholinergic cells such as hematopoietic and osteogenic cells. We have recently reported that AChE is expressed in various cells undergoing apoptosis. To characterize AChE in apoptotic cells and to investigate the role of AChE expression in apoptosis, we devised a method to purify AChE expressed in apoptotic human lung fibroblast cell line HLF. The isolation of this enzyme is mainly based on inhibitor ligand affinity chromatography using immobilized tacrine. However, this method is only effective in isolating active AChE. Here we employed antibody-based chromatography and found that both active and inactive AChE were present in apoptotic HLF cells. Active AChE was predominantly observed in the nuclei of apoptotic cells, while inactive AChE was mainly present in the cytoplasm. Therefore, our method provides an opportunity to investigate further the role of AChE, especially inactive AChE, in apoptosis." @default.
- W1930968 created "2016-06-24" @default.
- W1930968 creator A5011105439 @default.
- W1930968 creator A5011402397 @default.
- W1930968 creator A5017580443 @default.
- W1930968 creator A5024265672 @default.
- W1930968 creator A5030183640 @default.
- W1930968 creator A5039496582 @default.
- W1930968 creator A5060243058 @default.
- W1930968 creator A5062682623 @default.
- W1930968 date "2002-10-01" @default.
- W1930968 modified "2023-10-18" @default.
- W1930968 title "Isolation of Acetylcholinesterase from Apoptotic Human Lung Fibroblast Cells by Antibody Affinity Chromatography" @default.
- W1930968 cites W1494255360 @default.
- W1930968 cites W1510064740 @default.
- W1930968 cites W1540724623 @default.
- W1930968 cites W1557084204 @default.
- W1930968 cites W1562398969 @default.
- W1930968 cites W1971048984 @default.
- W1930968 cites W1971133960 @default.
- W1930968 cites W1971878065 @default.
- W1930968 cites W1977592017 @default.
- W1930968 cites W1988491247 @default.
- W1930968 cites W1991086071 @default.
- W1930968 cites W2004681649 @default.
- W1930968 cites W2010110673 @default.
- W1930968 cites W2011591323 @default.
- W1930968 cites W2017958468 @default.
- W1930968 cites W2019074857 @default.
- W1930968 cites W2019117032 @default.
- W1930968 cites W2044403686 @default.
- W1930968 cites W2046111418 @default.
- W1930968 cites W2066874647 @default.
- W1930968 cites W2067687039 @default.
- W1930968 cites W2073852483 @default.
- W1930968 cites W2077213380 @default.
- W1930968 cites W2083551340 @default.
- W1930968 cites W2086458951 @default.
- W1930968 cites W2090595766 @default.
- W1930968 cites W2092439492 @default.
- W1930968 cites W2100837269 @default.
- W1930968 cites W2104606868 @default.
- W1930968 cites W2118377154 @default.
- W1930968 cites W2119125561 @default.
- W1930968 cites W2125778456 @default.
- W1930968 cites W2134418761 @default.
- W1930968 cites W2137037469 @default.
- W1930968 cites W2159005901 @default.
- W1930968 cites W2254514044 @default.
- W1930968 cites W2317677291 @default.
- W1930968 cites W2329231956 @default.
- W1930968 cites W2436409688 @default.
- W1930968 doi "https://doi.org/10.2144/oct0211" @default.
- W1930968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12395932" @default.
- W1930968 hasPublicationYear "2002" @default.
- W1930968 type Work @default.
- W1930968 sameAs 1930968 @default.
- W1930968 citedByCount "5" @default.
- W1930968 countsByYear W19309682012 @default.
- W1930968 crossrefType "journal-article" @default.
- W1930968 hasAuthorship W1930968A5011105439 @default.
- W1930968 hasAuthorship W1930968A5011402397 @default.
- W1930968 hasAuthorship W1930968A5017580443 @default.
- W1930968 hasAuthorship W1930968A5024265672 @default.
- W1930968 hasAuthorship W1930968A5030183640 @default.
- W1930968 hasAuthorship W1930968A5039496582 @default.
- W1930968 hasAuthorship W1930968A5060243058 @default.
- W1930968 hasAuthorship W1930968A5062682623 @default.
- W1930968 hasBestOaLocation W19309681 @default.
- W1930968 hasConcept C134018914 @default.
- W1930968 hasConcept C153911025 @default.
- W1930968 hasConcept C179247698 @default.
- W1930968 hasConcept C181199279 @default.
- W1930968 hasConcept C185592680 @default.
- W1930968 hasConcept C190283241 @default.
- W1930968 hasConcept C22885893 @default.
- W1930968 hasConcept C2775910092 @default.
- W1930968 hasConcept C2778816929 @default.
- W1930968 hasConcept C2779046751 @default.
- W1930968 hasConcept C2779059548 @default.
- W1930968 hasConcept C54355233 @default.
- W1930968 hasConcept C55493867 @default.
- W1930968 hasConcept C81885089 @default.
- W1930968 hasConcept C86803240 @default.
- W1930968 hasConcept C95444343 @default.
- W1930968 hasConcept C98274493 @default.
- W1930968 hasConceptScore W1930968C134018914 @default.
- W1930968 hasConceptScore W1930968C153911025 @default.
- W1930968 hasConceptScore W1930968C179247698 @default.
- W1930968 hasConceptScore W1930968C181199279 @default.
- W1930968 hasConceptScore W1930968C185592680 @default.
- W1930968 hasConceptScore W1930968C190283241 @default.
- W1930968 hasConceptScore W1930968C22885893 @default.
- W1930968 hasConceptScore W1930968C2775910092 @default.
- W1930968 hasConceptScore W1930968C2778816929 @default.
- W1930968 hasConceptScore W1930968C2779046751 @default.
- W1930968 hasConceptScore W1930968C2779059548 @default.
- W1930968 hasConceptScore W1930968C54355233 @default.