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• W193111756 abstract "Engineering at the scale of whole genomes requires fundamentally new molecular biology tools. Recent advances in recombineering using synthetic oligonucleotides enable the rapid generation of mutants at high efficiency and specificity and can be implemented at the genome scale. With these techniques, libraries of mutants can be generated, from which individuals with functionally useful phenotypes can be isolated. Furthermore, populations of cells can be evolved in situ by directed evolution using complex pools of oligonucleotides. Here, we discuss ways to utilize these multiplexed genome engineering methods, with special emphasis on experimental design and implementation." @default.
• W193111756 created "2016-06-24" @default.
• W193111756 creator A5011136095 @default.
• W193111756 creator A5058824230 @default.
• W193111756 date "2011-01-01" @default.
• W193111756 modified "2023-10-11" @default.
• W193111756 title "Multiplexed Genome Engineering and Genotyping Methods" @default.
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• W193111756 doi "https://doi.org/10.1016/b978-0-12-385120-8.00018-8" @default.
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