Matches in SemOpenAlex for { <https://semopenalex.org/work/W1932131567> ?p ?o ?g. }
- W1932131567 endingPage "1626" @default.
- W1932131567 startingPage "1615" @default.
- W1932131567 abstract "Crohn's disease (CD) is an inflammatory bowel disease characterized by an exaggerated immune response to commensal microbiota in the intestines of patients. Metagenomic studies have identified specific bacterial species and strains with increased prevalence in CD patients, amongst which is the adherent-invasive Escherichia coli (AIEC) strain LF82. AIEC strains express long polar fimbriae (LPF), which are known to target Peyer's patches in a mouse CD model. Here, the recombinant production of a soluble, self-complemented construct of the LpfD protein of E. coli LF82 is reported and it is demonstrated that it forms the adhesive tip subunit of LPF. The LpfD crystal reveals an N-terminal adhesin domain and a C-terminal pilin domain that connects the adhesin to the minor pilus subunit LpfE. Surface topology and sequence conservation in the adhesin domain hint at a putative receptor-binding pocket as found in the Klebsiella pneumoniae MrkD and E. coli F17-G (GafD) adhesins. Immunohistostaining of murine intestinal tissue sections revealed that LpfD specifically binds to the intestinal mucosa and submucosa. LpfD binding was found to be resistant to treatment with O- or N-glycosidases, but was lost in collagenase-treated tissue sections, indicating the possible involvement of an intestinal matrix-associated protein as the LpfD receptor. LpfD strongly adhered to isolated fibronectin in an in vitro assay, and showed lower levels of binding to collagen V and laminin and no binding to collagens I, III and IV." @default.
- W1932131567 created "2016-06-24" @default.
- W1932131567 creator A5002784436 @default.
- W1932131567 creator A5026194678 @default.
- W1932131567 creator A5030787409 @default.
- W1932131567 creator A5035569738 @default.
- W1932131567 creator A5042484807 @default.
- W1932131567 creator A5046567495 @default.
- W1932131567 creator A5058330979 @default.
- W1932131567 creator A5088416076 @default.
- W1932131567 date "2015-07-28" @default.
- W1932131567 modified "2023-09-27" @default.
- W1932131567 title "Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive<i>Escherichia coli</i>" @default.
- W1932131567 cites W1607436257 @default.
- W1932131567 cites W1850372875 @default.
- W1932131567 cites W1967217097 @default.
- W1932131567 cites W1970800726 @default.
- W1932131567 cites W1973506442 @default.
- W1932131567 cites W1979845898 @default.
- W1932131567 cites W1979859398 @default.
- W1932131567 cites W199243767 @default.
- W1932131567 cites W2000511735 @default.
- W1932131567 cites W2004260774 @default.
- W1932131567 cites W2004377868 @default.
- W1932131567 cites W2008649826 @default.
- W1932131567 cites W2023783511 @default.
- W1932131567 cites W2024719744 @default.
- W1932131567 cites W2027609863 @default.
- W1932131567 cites W2030364983 @default.
- W1932131567 cites W2030517878 @default.
- W1932131567 cites W2035503835 @default.
- W1932131567 cites W2036629065 @default.
- W1932131567 cites W2040328437 @default.
- W1932131567 cites W2042588480 @default.
- W1932131567 cites W2042603611 @default.
- W1932131567 cites W2051369945 @default.
- W1932131567 cites W2052435488 @default.
- W1932131567 cites W2064032680 @default.
- W1932131567 cites W2066148427 @default.
- W1932131567 cites W2076121111 @default.
- W1932131567 cites W2080156786 @default.
- W1932131567 cites W2084677920 @default.
- W1932131567 cites W2090646040 @default.
- W1932131567 cites W2090964053 @default.
- W1932131567 cites W2097875133 @default.
- W1932131567 cites W2099125075 @default.
- W1932131567 cites W2101653954 @default.
- W1932131567 cites W2103459268 @default.
- W1932131567 cites W2103760724 @default.
- W1932131567 cites W2105362838 @default.
- W1932131567 cites W2107805776 @default.
- W1932131567 cites W2110223162 @default.
- W1932131567 cites W2115207445 @default.
- W1932131567 cites W2115453381 @default.
- W1932131567 cites W2117512031 @default.
- W1932131567 cites W2120950548 @default.
- W1932131567 cites W2122746216 @default.
- W1932131567 cites W2124026197 @default.
- W1932131567 cites W2130900937 @default.
- W1932131567 cites W2131350133 @default.
- W1932131567 cites W2131446724 @default.
- W1932131567 cites W2132926880 @default.
- W1932131567 cites W2136711544 @default.
- W1932131567 cites W2142069562 @default.
- W1932131567 cites W2146256775 @default.
- W1932131567 cites W2148118122 @default.
- W1932131567 cites W2149594409 @default.
- W1932131567 cites W2154907639 @default.
- W1932131567 cites W2155443248 @default.
- W1932131567 cites W2156145269 @default.
- W1932131567 cites W2158266834 @default.
- W1932131567 cites W2159211495 @default.
- W1932131567 cites W2159976230 @default.
- W1932131567 cites W2161244514 @default.
- W1932131567 cites W2180229411 @default.
- W1932131567 cites W2396688407 @default.
- W1932131567 cites W4248872320 @default.
- W1932131567 doi "https://doi.org/10.1107/s1399004715009803" @default.
- W1932131567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26249343" @default.
- W1932131567 hasPublicationYear "2015" @default.
- W1932131567 type Work @default.
- W1932131567 sameAs 1932131567 @default.
- W1932131567 citedByCount "8" @default.
- W1932131567 countsByYear W19321315672016 @default.
- W1932131567 countsByYear W19321315672017 @default.
- W1932131567 countsByYear W19321315672019 @default.
- W1932131567 countsByYear W19321315672022 @default.
- W1932131567 crossrefType "journal-article" @default.
- W1932131567 hasAuthorship W1932131567A5002784436 @default.
- W1932131567 hasAuthorship W1932131567A5026194678 @default.
- W1932131567 hasAuthorship W1932131567A5030787409 @default.
- W1932131567 hasAuthorship W1932131567A5035569738 @default.
- W1932131567 hasAuthorship W1932131567A5042484807 @default.
- W1932131567 hasAuthorship W1932131567A5046567495 @default.
- W1932131567 hasAuthorship W1932131567A5058330979 @default.
- W1932131567 hasAuthorship W1932131567A5088416076 @default.
- W1932131567 hasBestOaLocation W19321315672 @default.
- W1932131567 hasConcept C104292427 @default.