Matches in SemOpenAlex for { <https://semopenalex.org/work/W1932325> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1932325 endingPage "1307" @default.
- W1932325 startingPage "1295" @default.
- W1932325 abstract "3-Isopropylmalate dehydrogenase (IPMDH) from Mycobacterium tuberculosis (Mtb) may be a target for specific drugs against this pathogenic bacterium. We have expressed and purified Mtb IPMDH and determined its physicalchemical and enzymological properties. Size-exclusion chromatography and dynamic light scattering measurements (DLS) suggest a tetrameric structure for Mtb IPMDH, in contrast to the dimeric structure of most IPMDHs. The kinetic properties (k cat and K m values) of Mtb IPMDH and the pH-dependence of k cat are very similar to both Escherichia coli (Ec) and Thermus thermophilus (Tt) IPMDHs. The stability of Mtb IPMDH in 8 M urea is close to that of the mesophilic counterpart, Ec IPMDH, both of them being much less stable than the thermophilic (Tt) enzyme. Two known IPMDH inhibitors, O-methyl oxalohydroxamate and 3-methylmercaptomalate, have been synthesised. Their inhibitory effects were found to be independent of the origin of IPMDHs. Thus, experiments with either Ec or Tt IPMDH would be equally relevant for designing specific inhibitory drugs against Mtb IPMDH." @default.
- W1932325 created "2016-06-24" @default.
- W1932325 creator A5002194633 @default.
- W1932325 creator A5012234932 @default.
- W1932325 creator A5036198184 @default.
- W1932325 creator A5037863743 @default.
- W1932325 creator A5039477065 @default.
- W1932325 creator A5054730556 @default.
- W1932325 creator A5055602019 @default.
- W1932325 creator A5065944362 @default.
- W1932325 creator A5072228095 @default.
- W1932325 creator A5088140112 @default.
- W1932325 creator A5090705771 @default.
- W1932325 date "2014-11-05" @default.
- W1932325 modified "2023-10-06" @default.
- W1932325 title "Drugs Against Mycobacterium tuberculosis 3-Isopropylmalate Dehydrogenase Can be Developed Using Homologous Enzymes as Surrogate Targets" @default.
- W1932325 doi "https://doi.org/10.2174/0929866521666140606111019" @default.
- W1932325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24909230" @default.
- W1932325 hasPublicationYear "2014" @default.
- W1932325 type Work @default.
- W1932325 sameAs 1932325 @default.
- W1932325 citedByCount "2" @default.
- W1932325 countsByYear W19323252016 @default.
- W1932325 countsByYear W19323252017 @default.
- W1932325 crossrefType "journal-article" @default.
- W1932325 hasAuthorship W1932325A5002194633 @default.
- W1932325 hasAuthorship W1932325A5012234932 @default.
- W1932325 hasAuthorship W1932325A5036198184 @default.
- W1932325 hasAuthorship W1932325A5037863743 @default.
- W1932325 hasAuthorship W1932325A5039477065 @default.
- W1932325 hasAuthorship W1932325A5054730556 @default.
- W1932325 hasAuthorship W1932325A5055602019 @default.
- W1932325 hasAuthorship W1932325A5065944362 @default.
- W1932325 hasAuthorship W1932325A5072228095 @default.
- W1932325 hasAuthorship W1932325A5088140112 @default.
- W1932325 hasAuthorship W1932325A5090705771 @default.
- W1932325 hasConcept C104317684 @default.
- W1932325 hasConcept C142724271 @default.
- W1932325 hasConcept C181199279 @default.
- W1932325 hasConcept C185592680 @default.
- W1932325 hasConcept C2776317432 @default.
- W1932325 hasConcept C2777975735 @default.
- W1932325 hasConcept C2780883830 @default.
- W1932325 hasConcept C2781069245 @default.
- W1932325 hasConcept C27911776 @default.
- W1932325 hasConcept C523546767 @default.
- W1932325 hasConcept C54355233 @default.
- W1932325 hasConcept C547475151 @default.
- W1932325 hasConcept C55493867 @default.
- W1932325 hasConcept C63223902 @default.
- W1932325 hasConcept C71924100 @default.
- W1932325 hasConcept C86803240 @default.
- W1932325 hasConcept C89423630 @default.
- W1932325 hasConceptScore W1932325C104317684 @default.
- W1932325 hasConceptScore W1932325C142724271 @default.
- W1932325 hasConceptScore W1932325C181199279 @default.
- W1932325 hasConceptScore W1932325C185592680 @default.
- W1932325 hasConceptScore W1932325C2776317432 @default.
- W1932325 hasConceptScore W1932325C2777975735 @default.
- W1932325 hasConceptScore W1932325C2780883830 @default.
- W1932325 hasConceptScore W1932325C2781069245 @default.
- W1932325 hasConceptScore W1932325C27911776 @default.
- W1932325 hasConceptScore W1932325C523546767 @default.
- W1932325 hasConceptScore W1932325C54355233 @default.
- W1932325 hasConceptScore W1932325C547475151 @default.
- W1932325 hasConceptScore W1932325C55493867 @default.
- W1932325 hasConceptScore W1932325C63223902 @default.
- W1932325 hasConceptScore W1932325C71924100 @default.
- W1932325 hasConceptScore W1932325C86803240 @default.
- W1932325 hasConceptScore W1932325C89423630 @default.
- W1932325 hasIssue "12" @default.
- W1932325 hasLocation W19323251 @default.
- W1932325 hasLocation W19323252 @default.
- W1932325 hasOpenAccess W1932325 @default.
- W1932325 hasPrimaryLocation W19323251 @default.
- W1932325 hasRelatedWork W1529800611 @default.
- W1932325 hasRelatedWork W1972665833 @default.
- W1932325 hasRelatedWork W1983238175 @default.
- W1932325 hasRelatedWork W2026694947 @default.
- W1932325 hasRelatedWork W2085280324 @default.
- W1932325 hasRelatedWork W2140131252 @default.
- W1932325 hasRelatedWork W2347804804 @default.
- W1932325 hasRelatedWork W3088109935 @default.
- W1932325 hasRelatedWork W4306249549 @default.
- W1932325 hasRelatedWork W991386826 @default.
- W1932325 hasVolume "21" @default.
- W1932325 isParatext "false" @default.
- W1932325 isRetracted "false" @default.
- W1932325 magId "1932325" @default.
- W1932325 workType "article" @default.