Matches in SemOpenAlex for { <https://semopenalex.org/work/W1932857776> ?p ?o ?g. }
- W1932857776 endingPage "1685" @default.
- W1932857776 startingPage "1673" @default.
- W1932857776 abstract "Abstract The traditional NMR‐based method for determining oligomeric protein structure usually involves distinguishing and assigning intra‐ and intersubunit NOEs. This task becomes challenging when determining symmetric homo‐dimer structures because NOE cross‐peaks from a given pair of protons occur at the same position whether intra‐ or intersubunit in origin. While there are isotope‐filtering strategies for distinguishing intra from intermolecular NOE interactions in these cases, they are laborious and often prove ineffectual in cases of weak dimers, where observation of intermolecular NOEs is rare. Here, we present an efficient procedure for weak dimer structure determination based on residual dipolar couplings (RDCs), chemical shift changes upon dilution, and paramagnetic surface perturbations. This procedure is applied to the Northeast Structural Genomics Consortium protein target, SeR13, a negatively charged Staphylococcus epidermidis dimeric protein ( K d 3.4 ± 1.4 m M ) composed of 86 amino acids. A structure determination for the monomeric form using traditional NMR methods is presented, followed by a dimer structure determination using docking under orientation constraints from RDCs data, and scoring under residue pair potentials and shape‐based predictions of RDCs. Validation using paramagnetic surface perturbation and chemical shift perturbation data acquired on sample dilution is also presented. The general utility of the dimer structure determination procedure and the possible relevance of SeR13 dimer formation are discussed." @default.
- W1932857776 created "2016-06-24" @default.
- W1932857776 creator A5001790150 @default.
- W1932857776 creator A5009084253 @default.
- W1932857776 creator A5021128260 @default.
- W1932857776 creator A5031565874 @default.
- W1932857776 creator A5044542421 @default.
- W1932857776 creator A5047323502 @default.
- W1932857776 creator A5049161457 @default.
- W1932857776 creator A5055630954 @default.
- W1932857776 creator A5056101043 @default.
- W1932857776 date "2010-08-23" @default.
- W1932857776 modified "2023-09-25" @default.
- W1932857776 title "Three-dimensional structure of the weakly associated protein homodimer SeR13 using RDCs and paramagnetic surface mapping" @default.
- W1932857776 cites W1589295940 @default.
- W1932857776 cites W1691646448 @default.
- W1932857776 cites W1968868342 @default.
- W1932857776 cites W1970419892 @default.
- W1932857776 cites W1970724931 @default.
- W1932857776 cites W1975705094 @default.
- W1932857776 cites W1977845138 @default.
- W1932857776 cites W1981653396 @default.
- W1932857776 cites W1983851480 @default.
- W1932857776 cites W1985133799 @default.
- W1932857776 cites W1988110964 @default.
- W1932857776 cites W1991285104 @default.
- W1932857776 cites W1993292677 @default.
- W1932857776 cites W1997566863 @default.
- W1932857776 cites W1998417052 @default.
- W1932857776 cites W1999234644 @default.
- W1932857776 cites W2013154867 @default.
- W1932857776 cites W2013728992 @default.
- W1932857776 cites W2019163268 @default.
- W1932857776 cites W2027228214 @default.
- W1932857776 cites W2029667189 @default.
- W1932857776 cites W2030433731 @default.
- W1932857776 cites W2032981401 @default.
- W1932857776 cites W2037627497 @default.
- W1932857776 cites W2043073749 @default.
- W1932857776 cites W2046557655 @default.
- W1932857776 cites W2046683144 @default.
- W1932857776 cites W204911951 @default.
- W1932857776 cites W2052933818 @default.
- W1932857776 cites W2053202752 @default.
- W1932857776 cites W2058157826 @default.
- W1932857776 cites W2062557777 @default.
- W1932857776 cites W2065198366 @default.
- W1932857776 cites W2065554849 @default.
- W1932857776 cites W2067548892 @default.
- W1932857776 cites W2068078209 @default.
- W1932857776 cites W2068227670 @default.
- W1932857776 cites W2070189028 @default.
- W1932857776 cites W2076033623 @default.
- W1932857776 cites W2093329141 @default.
- W1932857776 cites W2093400164 @default.
- W1932857776 cites W2093482724 @default.
- W1932857776 cites W2098613471 @default.
- W1932857776 cites W2106092694 @default.
- W1932857776 cites W2107572237 @default.
- W1932857776 cites W2130037714 @default.
- W1932857776 cites W2143825685 @default.
- W1932857776 cites W2150981663 @default.
- W1932857776 cites W2152764495 @default.
- W1932857776 cites W2153371171 @default.
- W1932857776 cites W2165046172 @default.
- W1932857776 cites W2169821755 @default.
- W1932857776 cites W2170671445 @default.
- W1932857776 cites W2607529982 @default.
- W1932857776 doi "https://doi.org/10.1002/pro.447" @default.
- W1932857776 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2975131" @default.
- W1932857776 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20589905" @default.
- W1932857776 hasPublicationYear "2010" @default.
- W1932857776 type Work @default.
- W1932857776 sameAs 1932857776 @default.
- W1932857776 citedByCount "19" @default.
- W1932857776 countsByYear W19328577762013 @default.
- W1932857776 countsByYear W19328577762015 @default.
- W1932857776 countsByYear W19328577762016 @default.
- W1932857776 countsByYear W19328577762017 @default.
- W1932857776 countsByYear W19328577762018 @default.
- W1932857776 countsByYear W19328577762020 @default.
- W1932857776 countsByYear W19328577762022 @default.
- W1932857776 countsByYear W19328577762023 @default.
- W1932857776 crossrefType "journal-article" @default.
- W1932857776 hasAuthorship W1932857776A5001790150 @default.
- W1932857776 hasAuthorship W1932857776A5009084253 @default.
- W1932857776 hasAuthorship W1932857776A5021128260 @default.
- W1932857776 hasAuthorship W1932857776A5031565874 @default.
- W1932857776 hasAuthorship W1932857776A5044542421 @default.
- W1932857776 hasAuthorship W1932857776A5047323502 @default.
- W1932857776 hasAuthorship W1932857776A5049161457 @default.
- W1932857776 hasAuthorship W1932857776A5055630954 @default.
- W1932857776 hasAuthorship W1932857776A5056101043 @default.
- W1932857776 hasBestOaLocation W19328577762 @default.
- W1932857776 hasConcept C111429119 @default.
- W1932857776 hasConcept C112887158 @default.
- W1932857776 hasConcept C121332964 @default.
- W1932857776 hasConcept C124712363 @default.