FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1933704614> ?p ?o ?g. }

• W1933704614 endingPage "2727" @default.
• W1933704614 startingPage "2716" @default.
• W1933704614 abstract "The alc gene product (gpalc) of bacteriophage T4 inhibits the transcription of cytosine-containing DNA in vivo. We examined its effect on transcription in vitro by comparing RNA polymerase isolated from Escherichia coli infected with either wild-type T4D+ or alc mutants. A 50 to 60% decline in RNA polymerase activity, measured on phage T7 DNA, was observed by 1 min after infection with either T4D+ or alc mutants; this did not occur when the infecting phage lacked gpalt. In the case of the T4D+ strain but not alc mutants, this was followed by a further decrease. By 5 min after infection the activity of alc mutants was 1.5 to 2.5 times greater than that of the wild type on various cytosine-containing DNA templates, whereas there was little or no difference in activity on T4 HMdC-DNA, in agreement with the in vivo specificity. Effects on transcript initiation and elongation were distinguished by using a T7 phage DNA template. Rifampin challenge, end-labeling with [gamma-32P]ATP, and selective initiation with a dinucleotide all indicate that the decreased in vitro activity of the wild-type polymerase relative to that of the alc mutants was due to inhibition of elongation, not to any difference in initiation rates. Wild-type (but not mutated) gpalc copurified with RNA polymerase on heparin agarose but not in subsequent steps. Immunoprecipitation of modified RNA polymerase also indicated that gpalc was not tightly bound to RNA polymerase intracellularly. It thus appears likely that gpalc inhibits transcript elongation on cytosine-containing DNA by interacting with actively transcribing core polymerase as a complex with the enzyme and cytosine-rich stretches of the template." @default.
• W1933704614 created "2016-06-24" @default.
• W1933704614 creator A5034300724 @default.
• W1933704614 creator A5056076266 @default.
• W1933704614 date "1990-05-01" @default.
• W1933704614 modified "2023-09-26" @default.
• W1933704614 title "Inhibition of transcription of cytosine-containing DNA in vitro by the alc gene product of bacteriophage T4" @default.
• W1933704614 cites W1486767369 @default.
• W1933704614 cites W1502717868 @default.
• W1933704614 cites W1523260723 @default.
• W1933704614 cites W1527595827 @default.
• W1933704614 cites W1549766776 @default.
• W1933704614 cites W1589911250 @default.
• W1933704614 cites W1603704933 @default.
• W1933704614 cites W1604655986 @default.
• W1933704614 cites W1674720470 @default.
• W1933704614 cites W1682321367 @default.
• W1933704614 cites W1754716242 @default.
• W1933704614 cites W1821446127 @default.
• W1933704614 cites W184098300 @default.
• W1933704614 cites W1886820898 @default.
• W1933704614 cites W1942571175 @default.
• W1933704614 cites W1960665849 @default.
• W1933704614 cites W1966669124 @default.
• W1933704614 cites W1970081885 @default.
• W1933704614 cites W1977224575 @default.
• W1933704614 cites W1979743483 @default.
• W1933704614 cites W1984637352 @default.
• W1933704614 cites W1984808592 @default.
• W1933704614 cites W1994350816 @default.
• W1933704614 cites W1995991332 @default.
• W1933704614 cites W1999036927 @default.
• W1933704614 cites W2001491378 @default.
• W1933704614 cites W2002841189 @default.
• W1933704614 cites W2004416191 @default.
• W1933704614 cites W2004836954 @default.
• W1933704614 cites W2006568501 @default.
• W1933704614 cites W2009966858 @default.
• W1933704614 cites W2018289835 @default.
• W1933704614 cites W2019953621 @default.
• W1933704614 cites W2030551247 @default.
• W1933704614 cites W2034630918 @default.
• W1933704614 cites W2063047151 @default.
• W1933704614 cites W2065930935 @default.
• W1933704614 cites W2080563499 @default.
• W1933704614 cites W2082757320 @default.
• W1933704614 cites W2083951909 @default.
• W1933704614 cites W2087566356 @default.
• W1933704614 cites W2098562102 @default.
• W1933704614 cites W2099298539 @default.
• W1933704614 cites W2100837269 @default.
• W1933704614 cites W2102154371 @default.
• W1933704614 cites W2110276 @default.
• W1933704614 cites W2114403157 @default.
• W1933704614 cites W2121469916 @default.
• W1933704614 cites W2129731624 @default.
• W1933704614 cites W2142900690 @default.
• W1933704614 cites W2156826925 @default.
• W1933704614 cites W2187600606 @default.
• W1933704614 cites W2245149899 @default.
• W1933704614 cites W2278385934 @default.
• W1933704614 cites W2403241400 @default.
• W1933704614 cites W4246577574 @default.
• W1933704614 cites W2097271294 @default.
• W1933704614 doi "https://doi.org/10.1128/jb.172.5.2716-2727.1990" @default.
• W1933704614 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/208917" @default.
• W1933704614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2185231" @default.
• W1933704614 hasPublicationYear "1990" @default.
• W1933704614 type Work @default.
• W1933704614 sameAs 1933704614 @default.
• W1933704614 citedByCount "18" @default.
• W1933704614 countsByYear W19337046142018 @default.
• W1933704614 countsByYear W19337046142021 @default.
• W1933704614 countsByYear W19337046142022 @default.
• W1933704614 countsByYear W19337046142023 @default.
• W1933704614 crossrefType "journal-article" @default.
• W1933704614 hasAuthorship W1933704614A5034300724 @default.
• W1933704614 hasAuthorship W1933704614A5056076266 @default.
• W1933704614 hasBestOaLocation W19337046141 @default.
• W1933704614 hasConcept C101762097 @default.
• W1933704614 hasConcept C104317684 @default.
• W1933704614 hasConcept C138885662 @default.
• W1933704614 hasConcept C143065580 @default.
• W1933704614 hasConcept C150194340 @default.
• W1933704614 hasConcept C153911025 @default.
• W1933704614 hasConcept C156719811 @default.
• W1933704614 hasConcept C179926584 @default.
• W1933704614 hasConcept C2756471 @default.
• W1933704614 hasConcept C2776023528 @default.
• W1933704614 hasConcept C2776441376 @default.
• W1933704614 hasConcept C2776449523 @default.
• W1933704614 hasConcept C41258723 @default.
• W1933704614 hasConcept C41895202 @default.
• W1933704614 hasConcept C547475151 @default.
• W1933704614 hasConcept C552990157 @default.
• W1933704614 hasConcept C55493867 @default.
• W1933704614 hasConcept C64350747 @default.
• W1933704614 hasConcept C67705224 @default.

• next