FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1934031021> ?p ?o ?g. }

• W1934031021 endingPage "7842" @default.
• W1934031021 startingPage "7835" @default.
• W1934031021 abstract "Abstract Based on its substrate specificity, activation-induced cytidine deaminase can directly induce C:G mutations in Ig genes. However the origin of A:T mutations, which occur in a similar proportion in germinal center (GC) B cells, is unclear. Genetic evidence suggests that the induction of A:T mutations requires the components of the mismatch repair system and DNA polymerase η (POLH). We found that fibroblasts and GC B cells expressed similar levels of the mismatch repair components, but nonetheless the fibroblasts failed to generate a significant proportion of A:T mutations in a GFP reporter gene even after POLH overexpression. To investigate whether the ability to generate A:T mutations is dependent on the cellular environment (i.e., GC B cell or fibroblast) or the target gene (i.e., Ig or GFP), we developed a mutation detection system in a human GC-like cell line. We introduced a GFP gene with a premature stop codon into Ramos cells and compared the activation-induced cytidine deaminase-induced mutations in the endogenous VH and the transgenic GFP genes. Remarkably, a high proportion of A:T mutations was induced in both genes. Ectopic expression of POLH did not further increase the proportion of A:T mutations but diminished the strand bias of these mutations that is normally observed in VH genes. Intriguingly, the total mutation frequency in the GFP gene was consistently one-fifth of that in the VH gene. These results demonstrate that the ability to generate A:T mutations is dependent on the GC B cell environment but independent of the mutation frequency and target gene location." @default.
• W1934031021 created "2016-06-24" @default.
• W1934031021 creator A5027023051 @default.
• W1934031021 creator A5028730285 @default.
• W1934031021 creator A5034824441 @default.
• W1934031021 creator A5049703919 @default.
• W1934031021 creator A5058904312 @default.
• W1934031021 creator A5060986259 @default.
• W1934031021 creator A5081005167 @default.
• W1934031021 date "2008-12-01" @default.
• W1934031021 modified "2023-10-12" @default.
• W1934031021 title "Induction of A:T Mutations Is Dependent on Cellular Environment but Independent of Mutation Frequency and Target Gene Location" @default.
• W1934031021 cites W1534189371 @default.
• W1934031021 cites W1560486125 @default.
• W1934031021 cites W1580707139 @default.
• W1934031021 cites W1965320850 @default.
• W1934031021 cites W1969301604 @default.
• W1934031021 cites W1985264665 @default.
• W1934031021 cites W1998079194 @default.
• W1934031021 cites W2002856294 @default.
• W1934031021 cites W2006859213 @default.
• W1934031021 cites W2023568503 @default.
• W1934031021 cites W2025028594 @default.
• W1934031021 cites W2025750579 @default.
• W1934031021 cites W2028649337 @default.
• W1934031021 cites W2029864255 @default.
• W1934031021 cites W2031549362 @default.
• W1934031021 cites W2036671653 @default.
• W1934031021 cites W2037404852 @default.
• W1934031021 cites W2038590416 @default.
• W1934031021 cites W2040137525 @default.
• W1934031021 cites W2045816796 @default.
• W1934031021 cites W2061265134 @default.
• W1934031021 cites W2063508680 @default.
• W1934031021 cites W2063596163 @default.
• W1934031021 cites W2067026554 @default.
• W1934031021 cites W2084939222 @default.
• W1934031021 cites W2094094914 @default.
• W1934031021 cites W2102129515 @default.
• W1934031021 cites W2104809534 @default.
• W1934031021 cites W2117178083 @default.
• W1934031021 cites W2119460195 @default.
• W1934031021 cites W2137754338 @default.
• W1934031021 cites W2142538175 @default.
• W1934031021 cites W2147191451 @default.
• W1934031021 cites W2157577917 @default.
• W1934031021 cites W2162206449 @default.
• W1934031021 cites W2165608602 @default.
• W1934031021 cites W2166316790 @default.
• W1934031021 cites W2168783267 @default.
• W1934031021 cites W2171690009 @default.
• W1934031021 doi "https://doi.org/10.4049/jimmunol.181.11.7835" @default.
• W1934031021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19017973" @default.
• W1934031021 hasPublicationYear "2008" @default.
• W1934031021 type Work @default.
• W1934031021 sameAs 1934031021 @default.
• W1934031021 citedByCount "8" @default.
• W1934031021 countsByYear W19340310212012 @default.
• W1934031021 countsByYear W19340310212013 @default.
• W1934031021 countsByYear W19340310212015 @default.
• W1934031021 countsByYear W19340310212017 @default.
• W1934031021 crossrefType "journal-article" @default.
• W1934031021 hasAuthorship W1934031021A5027023051 @default.
• W1934031021 hasAuthorship W1934031021A5028730285 @default.
• W1934031021 hasAuthorship W1934031021A5034824441 @default.
• W1934031021 hasAuthorship W1934031021A5049703919 @default.
• W1934031021 hasAuthorship W1934031021A5058904312 @default.
• W1934031021 hasAuthorship W1934031021A5060986259 @default.
• W1934031021 hasAuthorship W1934031021A5081005167 @default.
• W1934031021 hasBestOaLocation W19340310211 @default.
• W1934031021 hasConcept C102306862 @default.
• W1934031021 hasConcept C104317684 @default.
• W1934031021 hasConcept C128526571 @default.
• W1934031021 hasConcept C150194340 @default.
• W1934031021 hasConcept C153911025 @default.
• W1934031021 hasConcept C159654299 @default.
• W1934031021 hasConcept C161733203 @default.
• W1934031021 hasConcept C27082348 @default.
• W1934031021 hasConcept C2776985229 @default.
• W1934031021 hasConcept C2778453870 @default.
• W1934031021 hasConcept C501734568 @default.
• W1934031021 hasConcept C54355233 @default.
• W1934031021 hasConcept C86803240 @default.
• W1934031021 hasConceptScore W1934031021C102306862 @default.
• W1934031021 hasConceptScore W1934031021C104317684 @default.
• W1934031021 hasConceptScore W1934031021C128526571 @default.
• W1934031021 hasConceptScore W1934031021C150194340 @default.
• W1934031021 hasConceptScore W1934031021C153911025 @default.
• W1934031021 hasConceptScore W1934031021C159654299 @default.
• W1934031021 hasConceptScore W1934031021C161733203 @default.
• W1934031021 hasConceptScore W1934031021C27082348 @default.
• W1934031021 hasConceptScore W1934031021C2776985229 @default.
• W1934031021 hasConceptScore W1934031021C2778453870 @default.
• W1934031021 hasConceptScore W1934031021C501734568 @default.
• W1934031021 hasConceptScore W1934031021C54355233 @default.
• W1934031021 hasConceptScore W1934031021C86803240 @default.
• W1934031021 hasIssue "11" @default.
• W1934031021 hasLocation W19340310211 @default.

• next