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• W1934062495 abstract "Immunohistochemical localization of choline acetyltransferase (ChAT) in cholinergic neurons has been difficult to achieve because of problems encountered in producing specific antisera. Here we describe the production and characterization of several distinct monoclonal antibodies to ChAT. Each of the monoclonal antibodies exhibits one of three general patterns of cross-species reactions; one pattern shows reactivity limited mainly to bovine ChAT, a second pattern shows reactivity only to ChAT from higher mammals including humans, and the third pattern shows reactivity to ChAT from all mammals tested. The antibodies bound specifically to two closely related bovine proteins of 68,000 and 70,000 daltons using the Western blotting technique. One of the antibodies was used to localize immunohistochemically known cholinergic structures in the rat brain, including motor neurons, basal forebrain neurons, and neostriatal neurons." @default.
• W1934062495 created "2016-06-24" @default.
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• W1934062495 date "1983-01-01" @default.
• W1934062495 modified "2023-09-27" @default.
• W1934062495 title "Monoclonal antibodies to choline acetyltransferase: production, specificity, and immunohistochemistry" @default.
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• W1934062495 doi "https://doi.org/10.1523/jneurosci.03-01-00001.1983" @default.
• W1934062495 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6564589" @default.
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