FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1934632378> ?p ?o ?g. }

• W1934632378 endingPage "4763" @default.
• W1934632378 startingPage "4763" @default.
• W1934632378 abstract "Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and subcellular localization Maxwell Korang-Yeboah,1 Yamini Gorantla,1 Simon A Paulos,1 Pankaj Sharma,2 Jaideep Chaudhary,2 Ravi Palaniappan1 1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Mercer University, 2Center for Cancer Research and Therapeutic Development (CCRTD), Clark Atlanta University, Atlanta, GA, USA Abstract: Prostate cancer (PCa) disease progression is associated with significant changes in intracellular and extracellular proteins, intracellular signaling mechanism, and cancer cell phenotype. These changes may have direct impact on the cellular interactions with nanocarriers; hence, there is the need for a much-detailed understanding, as nanocarrier cellular internalization and intracellular sorting mechanism correlate directly with bioavailability and clinical efficacy. In this study, we report the differences in the rate and mechanism of cellular internalization of a biocompatible polycaprolactone (PCL)/maltodextrin (MD) nanocarrier system for intracellular drug delivery in LNCaP, PC3, and DU145 PCa cell lines. PCL/MD nanocarriers were designed and characterized. PCL/MD nanocarriers significantly increased the intracellular concentration of coumarin-6 and fluorescein isothiocyanate-labeled bovine serum albumin, a model hydrophobic and large molecule, respectively. Fluorescence microscopy and flow cytometry analysis revealed rapid internalization of the nanocarrier. The extent of nanocarrier cellular internalization correlated directly with cell line aggressiveness. PCL/MD internalization was highest in PC3 followed by DU145 and LNCaP, respectively. Uptake in all PCa cell lines was metabolically dependent. Extraction of endogenous cholesterol by methyl-ß-cyclodextrin reduced uptake by 75%±4.53% in PC3, 64%±6.01% in LNCaP, and 50%±4.50% in DU145, indicating the involvement of endogenous cholesterol in cellular internalization. Internalization of the nanocarrier in LNCaP was mediated mainly by macropinocytosis and clathrin-independent pathways, while internalization in PC3 and DU145 involved clathrin-mediated endocytosis, clathrin-independent pathways, and macropinocytosis. Fluorescence microscopy showed a very diffused and non-compartmentalized subcellular localization of the PCL/MD nanocarriers with possible intranuclear localization and minor colocalization in the lysosomes with time. Keywords: endocytosis, prostate cancer, subcellular targeting, macropinocytosis, clathrin-mediated endocytosis&nbsp" @default.
• W1934632378 created "2016-06-24" @default.
• W1934632378 creator A5015607835 @default.
• W1934632378 creator A5025834401 @default.
• W1934632378 creator A5027958167 @default.
• W1934632378 creator A5072992833 @default.
• W1934632378 creator A5078510108 @default.
• W1934632378 creator A5089117285 @default.
• W1934632378 date "2015-07-01" @default.
• W1934632378 modified "2023-09-26" @default.
• W1934632378 title "Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and&nbsp;subcellular localization" @default.
• W1934632378 cites W1319875 @default.
• W1934632378 cites W138740573 @default.
• W1934632378 cites W1494596349 @default.
• W1934632378 cites W1536788210 @default.
• W1934632378 cites W1569789584 @default.
• W1934632378 cites W1958696553 @default.
• W1934632378 cites W1965530119 @default.
• W1934632378 cites W1971071816 @default.
• W1934632378 cites W1974683325 @default.
• W1934632378 cites W1975076384 @default.
• W1934632378 cites W1989353157 @default.
• W1934632378 cites W1995082650 @default.
• W1934632378 cites W1998344919 @default.
• W1934632378 cites W2001882385 @default.
• W1934632378 cites W2003110090 @default.
• W1934632378 cites W2003651291 @default.
• W1934632378 cites W2014058353 @default.
• W1934632378 cites W2015869127 @default.
• W1934632378 cites W2018653904 @default.
• W1934632378 cites W2025292917 @default.
• W1934632378 cites W2026975571 @default.
• W1934632378 cites W2030855909 @default.
• W1934632378 cites W2032760352 @default.
• W1934632378 cites W2034724750 @default.
• W1934632378 cites W2037564713 @default.
• W1934632378 cites W2039123138 @default.
• W1934632378 cites W2040174416 @default.
• W1934632378 cites W2043237370 @default.
• W1934632378 cites W2046890107 @default.
• W1934632378 cites W2050291058 @default.
• W1934632378 cites W2051776908 @default.
• W1934632378 cites W2055340135 @default.
• W1934632378 cites W2079687293 @default.
• W1934632378 cites W2079793032 @default.
• W1934632378 cites W2082511916 @default.
• W1934632378 cites W2082524595 @default.
• W1934632378 cites W2083982839 @default.
• W1934632378 cites W2086651095 @default.
• W1934632378 cites W2089863147 @default.
• W1934632378 cites W2089974427 @default.
• W1934632378 cites W2091342503 @default.
• W1934632378 cites W2097259163 @default.
• W1934632378 cites W2106220021 @default.
• W1934632378 cites W2107204091 @default.
• W1934632378 cites W2109502519 @default.
• W1934632378 cites W2110885272 @default.
• W1934632378 cites W2117630026 @default.
• W1934632378 cites W2129917557 @default.
• W1934632378 cites W2132236231 @default.
• W1934632378 cites W2134891510 @default.
• W1934632378 cites W2146237608 @default.
• W1934632378 cites W2152366705 @default.
• W1934632378 cites W2160271899 @default.
• W1934632378 cites W2163245582 @default.
• W1934632378 cites W2165580132 @default.
• W1934632378 cites W2172260616 @default.
• W1934632378 doi "https://doi.org/10.2147/ijn.s75101" @default.
• W1934632378 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4524459" @default.
• W1934632378 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26251597" @default.
• W1934632378 hasPublicationYear "2015" @default.
• W1934632378 type Work @default.
• W1934632378 sameAs 1934632378 @default.
• W1934632378 citedByCount "9" @default.
• W1934632378 countsByYear W19346323782016 @default.
• W1934632378 countsByYear W19346323782017 @default.
• W1934632378 countsByYear W19346323782018 @default.
• W1934632378 countsByYear W19346323782019 @default.
• W1934632378 countsByYear W19346323782021 @default.
• W1934632378 countsByYear W19346323782023 @default.
• W1934632378 crossrefType "journal-article" @default.
• W1934632378 hasAuthorship W1934632378A5015607835 @default.
• W1934632378 hasAuthorship W1934632378A5025834401 @default.
• W1934632378 hasAuthorship W1934632378A5027958167 @default.
• W1934632378 hasAuthorship W1934632378A5072992833 @default.
• W1934632378 hasAuthorship W1934632378A5078510108 @default.
• W1934632378 hasAuthorship W1934632378A5089117285 @default.
• W1934632378 hasBestOaLocation W19346323781 @default.
• W1934632378 hasConcept C121608353 @default.
• W1934632378 hasConcept C139770010 @default.
• W1934632378 hasConcept C1491633281 @default.
• W1934632378 hasConcept C178790620 @default.
• W1934632378 hasConcept C18150654 @default.
• W1934632378 hasConcept C185592680 @default.
• W1934632378 hasConcept C2776438761 @default.
• W1934632378 hasConcept C2779723316 @default.
• W1934632378 hasConcept C2779820397 @default.
• W1934632378 hasConcept C54355233 @default.

• next