Matches in SemOpenAlex for { <https://semopenalex.org/work/W1935789387> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1935789387 endingPage "1183" @default.
- W1935789387 startingPage "1177" @default.
- W1935789387 abstract "Immobilized subtilisin Novo was used for the cleavage of iron-saturated ovotransferrin (Fe2OT) into separate NH2- and CO2H-terminal iron-binding fragments, designated as FeNF and FeCF, respectively. The Mr of each fragment is 39,000. The purified fragments show major differences in the content of histidine, alanine, and methionine. Both apo-NH2- and apo-CO2H-terminal fragments are able to bind one ferric ion per molecule. FeCF is more resistant than FeNF to dissociation at acid pH and to subtilisin action. FeNF and FeCF are immunochemically distinct. However, equal mixtures of the two show immunochemical reaction indistinguishable from intact Fe2OT. The iron-binding sites of FeNF and FeCF are very similar to each other on the basis of visible absorption and CD spectra. The major difference in the backbone conformations between FeNF and FeCF is in the alpha-helical content of FeCF which is twice that of FeNF. Individually, fragments show quantitative differences in the electron paramagnetic resonance spectra; however, equal mixture of the two fragments produce EPR spectra very similar to that of the intact Fe2OT. These studies indicate that subtilitic cleavage of Fe2OT does not produce significant change in the iron-binding capacity or the conformation of the separated iron-binding domains." @default.
- W1935789387 created "2016-06-24" @default.
- W1935789387 creator A5032616570 @default.
- W1935789387 creator A5049836530 @default.
- W1935789387 creator A5063392149 @default.
- W1935789387 date "1982-02-01" @default.
- W1935789387 modified "2023-10-18" @default.
- W1935789387 title "Structure and function of ovotransferrin. I. Production of iron-binding fragments from iron-ovotransferrin by the action of immobilized subtilisin. Purification and characterization of the fragments." @default.
- W1935789387 cites W1419481270 @default.
- W1935789387 cites W1484115349 @default.
- W1935789387 cites W1499531688 @default.
- W1935789387 cites W1538207147 @default.
- W1935789387 cites W1541445884 @default.
- W1935789387 cites W1545622372 @default.
- W1935789387 cites W1572456528 @default.
- W1935789387 cites W1775749144 @default.
- W1935789387 cites W1826182428 @default.
- W1935789387 cites W1940669864 @default.
- W1935789387 cites W194912786 @default.
- W1935789387 cites W1995381450 @default.
- W1935789387 cites W1996617806 @default.
- W1935789387 cites W1999040086 @default.
- W1935789387 cites W2000855839 @default.
- W1935789387 cites W2011372320 @default.
- W1935789387 cites W2014550271 @default.
- W1935789387 cites W2017470279 @default.
- W1935789387 cites W2019465667 @default.
- W1935789387 cites W2027713145 @default.
- W1935789387 cites W2039598741 @default.
- W1935789387 cites W2045872281 @default.
- W1935789387 cites W2047540421 @default.
- W1935789387 cites W2055852963 @default.
- W1935789387 cites W2062228103 @default.
- W1935789387 cites W2073814416 @default.
- W1935789387 cites W2073879684 @default.
- W1935789387 cites W2111301002 @default.
- W1935789387 cites W2140333014 @default.
- W1935789387 cites W2238275288 @default.
- W1935789387 cites W2260568133 @default.
- W1935789387 cites W2282110776 @default.
- W1935789387 cites W2411811260 @default.
- W1935789387 cites W4211261432 @default.
- W1935789387 cites W52184915 @default.
- W1935789387 doi "https://doi.org/10.1016/s0021-9258(19)68171-8" @default.
- W1935789387 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6799501" @default.
- W1935789387 hasPublicationYear "1982" @default.
- W1935789387 type Work @default.
- W1935789387 sameAs 1935789387 @default.
- W1935789387 citedByCount "18" @default.
- W1935789387 countsByYear W19357893872013 @default.
- W1935789387 countsByYear W19357893872015 @default.
- W1935789387 crossrefType "journal-article" @default.
- W1935789387 hasAuthorship W1935789387A5032616570 @default.
- W1935789387 hasAuthorship W1935789387A5049836530 @default.
- W1935789387 hasAuthorship W1935789387A5063392149 @default.
- W1935789387 hasBestOaLocation W19357893871 @default.
- W1935789387 hasConcept C181199279 @default.
- W1935789387 hasConcept C185592680 @default.
- W1935789387 hasConcept C22814914 @default.
- W1935789387 hasConcept C2779811187 @default.
- W1935789387 hasConcept C2780223390 @default.
- W1935789387 hasConcept C43617362 @default.
- W1935789387 hasConcept C55493867 @default.
- W1935789387 hasConceptScore W1935789387C181199279 @default.
- W1935789387 hasConceptScore W1935789387C185592680 @default.
- W1935789387 hasConceptScore W1935789387C22814914 @default.
- W1935789387 hasConceptScore W1935789387C2779811187 @default.
- W1935789387 hasConceptScore W1935789387C2780223390 @default.
- W1935789387 hasConceptScore W1935789387C43617362 @default.
- W1935789387 hasConceptScore W1935789387C55493867 @default.
- W1935789387 hasIssue "3" @default.
- W1935789387 hasLocation W19357893871 @default.
- W1935789387 hasOpenAccess W1935789387 @default.
- W1935789387 hasPrimaryLocation W19357893871 @default.
- W1935789387 hasRelatedWork W1983836973 @default.
- W1935789387 hasRelatedWork W1996577413 @default.
- W1935789387 hasRelatedWork W2000878371 @default.
- W1935789387 hasRelatedWork W2033642355 @default.
- W1935789387 hasRelatedWork W2063635354 @default.
- W1935789387 hasRelatedWork W2067169110 @default.
- W1935789387 hasRelatedWork W2091142318 @default.
- W1935789387 hasRelatedWork W2159912536 @default.
- W1935789387 hasRelatedWork W397440131 @default.
- W1935789387 hasRelatedWork W4211068052 @default.
- W1935789387 hasVolume "257" @default.
- W1935789387 isParatext "false" @default.
- W1935789387 isRetracted "false" @default.
- W1935789387 magId "1935789387" @default.
- W1935789387 workType "article" @default.