Matches in SemOpenAlex for { <https://semopenalex.org/work/W1936352775> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1936352775 endingPage "32" @default.
- W1936352775 startingPage "32" @default.
- W1936352775 abstract "Giardia intestinalis is a unicellular parasite of worldwide distribution. It causes an intestinal illness known as giardiasis, and it is probably the earliest diverging eukaryotic microorganism. Previously, changes have been reported in the expression of mRNAs at several stages of the life cycle; however specific enzymatic activity changes have not been explored.The expression of pyruvate ferredoxin oxidoreductase (PFOR) and alcohol dehydrogenase E (ADHE) enzymes was measured in cyst and trophozoite stages, and during the excystation process.Recombinant proteins were generated for PFOR and ADHE to be used as antigens in the production of polyclonal antibodies for the detection of native proteins by Western Blot. The enzymatic activity of ADHE and glutamate dehydrogenase (GDH) was evaluated by spectrophotometric assays.PFOR (139 kDa) and ADHE (97 kDa) proteins were detected in trophozoites, but not in cysts. During excystation, ADHE protein was detected after the first phase of induction, but the PFOR protein appeared only after the second phase. This indicated that both proteins were synthesized during excystation, although at different times. ADHE enzymatic activity was present only in trophozoites and not in cysts whereas GDH activity was detected in both stages.These results conclusively showed that PFOR and ADHE enzymes were translated during the excystation process and is strong evidence that active protein synthesis was occurring during excystation." @default.
- W1936352775 created "2016-06-24" @default.
- W1936352775 creator A5084833787 @default.
- W1936352775 creator A5091766506 @default.
- W1936352775 date "2010-03-01" @default.
- W1936352775 modified "2023-10-11" @default.
- W1936352775 title "Síntesis de las enzimas de piruvato, oxidorreductasa de ferredoxina y deshidrogenasa E de alcohol durante el desenquistamiento (excystacion) de Giardia intestinalis" @default.
- W1936352775 cites W111244732 @default.
- W1936352775 cites W1969648719 @default.
- W1936352775 cites W1983785927 @default.
- W1936352775 cites W1984742638 @default.
- W1936352775 cites W1992543692 @default.
- W1936352775 cites W2017666097 @default.
- W1936352775 cites W2022562784 @default.
- W1936352775 cites W2030823707 @default.
- W1936352775 cites W2038287696 @default.
- W1936352775 cites W2057100489 @default.
- W1936352775 cites W2080055578 @default.
- W1936352775 cites W2138755616 @default.
- W1936352775 cites W2152977880 @default.
- W1936352775 cites W2335684512 @default.
- W1936352775 cites W2076982087 @default.
- W1936352775 doi "https://doi.org/10.7705/biomedica.v30i1.150" @default.
- W1936352775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20890547" @default.
- W1936352775 hasPublicationYear "2010" @default.
- W1936352775 type Work @default.
- W1936352775 sameAs 1936352775 @default.
- W1936352775 citedByCount "1" @default.
- W1936352775 countsByYear W19363527752014 @default.
- W1936352775 crossrefType "journal-article" @default.
- W1936352775 hasAuthorship W1936352775A5084833787 @default.
- W1936352775 hasAuthorship W1936352775A5091766506 @default.
- W1936352775 hasBestOaLocation W19363527751 @default.
- W1936352775 hasConcept C104317684 @default.
- W1936352775 hasConcept C147483822 @default.
- W1936352775 hasConcept C153911025 @default.
- W1936352775 hasConcept C170493617 @default.
- W1936352775 hasConcept C181199279 @default.
- W1936352775 hasConcept C185592680 @default.
- W1936352775 hasConcept C203014093 @default.
- W1936352775 hasConcept C2776415932 @default.
- W1936352775 hasConcept C2777202666 @default.
- W1936352775 hasConcept C2777271458 @default.
- W1936352775 hasConcept C2777344606 @default.
- W1936352775 hasConcept C2780626423 @default.
- W1936352775 hasConcept C55493867 @default.
- W1936352775 hasConcept C61174792 @default.
- W1936352775 hasConcept C86803240 @default.
- W1936352775 hasConcept C89423630 @default.
- W1936352775 hasConcept C956191 @default.
- W1936352775 hasConceptScore W1936352775C104317684 @default.
- W1936352775 hasConceptScore W1936352775C147483822 @default.
- W1936352775 hasConceptScore W1936352775C153911025 @default.
- W1936352775 hasConceptScore W1936352775C170493617 @default.
- W1936352775 hasConceptScore W1936352775C181199279 @default.
- W1936352775 hasConceptScore W1936352775C185592680 @default.
- W1936352775 hasConceptScore W1936352775C203014093 @default.
- W1936352775 hasConceptScore W1936352775C2776415932 @default.
- W1936352775 hasConceptScore W1936352775C2777202666 @default.
- W1936352775 hasConceptScore W1936352775C2777271458 @default.
- W1936352775 hasConceptScore W1936352775C2777344606 @default.
- W1936352775 hasConceptScore W1936352775C2780626423 @default.
- W1936352775 hasConceptScore W1936352775C55493867 @default.
- W1936352775 hasConceptScore W1936352775C61174792 @default.
- W1936352775 hasConceptScore W1936352775C86803240 @default.
- W1936352775 hasConceptScore W1936352775C89423630 @default.
- W1936352775 hasConceptScore W1936352775C956191 @default.
- W1936352775 hasIssue "1" @default.
- W1936352775 hasLocation W19363527751 @default.
- W1936352775 hasLocation W19363527752 @default.
- W1936352775 hasLocation W19363527753 @default.
- W1936352775 hasOpenAccess W1936352775 @default.
- W1936352775 hasPrimaryLocation W19363527751 @default.
- W1936352775 hasRelatedWork W1515301467 @default.
- W1936352775 hasRelatedWork W17100580 @default.
- W1936352775 hasRelatedWork W2234375112 @default.
- W1936352775 hasRelatedWork W2349462460 @default.
- W1936352775 hasRelatedWork W2350566209 @default.
- W1936352775 hasRelatedWork W2355979262 @default.
- W1936352775 hasRelatedWork W2359256335 @default.
- W1936352775 hasRelatedWork W2377259361 @default.
- W1936352775 hasRelatedWork W2388642114 @default.
- W1936352775 hasRelatedWork W2394407329 @default.
- W1936352775 hasVolume "30" @default.
- W1936352775 isParatext "false" @default.
- W1936352775 isRetracted "false" @default.
- W1936352775 magId "1936352775" @default.
- W1936352775 workType "article" @default.