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- W193640207 abstract "The Candida spp. are emerging opportunistic pathogens. Phagocytic cells are a primary line of defense against these opportunistic pathogens. Upon phagocytosis by macrophages, C. albicans reprograms its metabolism because genes involved in the peroxisomal metabolism, such as glyoxylic acid cycle and beta-oxidation pathway, are overexpressed. The objective of this study was to study the relation between fatty acid beta-oxidation, resistance to phagocytosis and virulence in the biological model Candida lusitaniae. In ascomycetous yeasts, the fatty acid β-oxidation is assumed to be exclusively located to peroxisomes.We constructed three null-mutants in C. lusitaniae: icl1Δ, fox2Δ et pxa1Δ, respectively lacking the isocitrate lyase (a key enzyme of the glyoxylate cycle), the multifunctional fatty acid beta-oxidation protein (essential in C. albicans to the β-oxidation pathway), and a protein involved in the peroxisomal import of long-chain fatty acids. The study of fatty acid assimilation and 14Calpha-palmitoyl-CoA catabolism revealed that long-chain fatty acids were still catabolized in fox2Δ. The observation of 14Calpha-palmitoyl-CoA catabolism in mitochondrial and peroxisomal fractions of wild-type and fox2Δ strains, the immunolocalization of Fox2p and mitochondrial respiration measurements yielded to the first demonstration in ascomycetous yeast of a mitochondrial Fox2p-dependent fatty acid β-oxidation pathway. We also demonstrated for the first time in Eucaryota that Fox2p co-localized in both peroxisomes and mitochondria. The invalidation of FOX1 and FOX3 genes (acyl-CoA oxidase and ketoacyl-CoA thiolase, respectively) confirmed for the first time in Fungi the existence of an alternative peroxisomal pathway for fatty acid catabolism, Fox2p-independently." @default.
- W193640207 created "2016-06-24" @default.
- W193640207 creator A5016941107 @default.
- W193640207 date "2011-12-15" @default.
- W193640207 modified "2023-09-27" @default.
- W193640207 title "Etude fonctionnelle de la β-oxydation chez la levure pathogène opportuniste Candida lusitaniae : caractérisation d’une voie mitochondriale et peroxysomale Fox2p-dépendante et mise en évidence d’une voie peroxysomale alternative Fox2p-indépendante de catabolisme des acides gras" @default.
- W193640207 hasPublicationYear "2011" @default.
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