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• W193644956 endingPage "90" @default.
• W193644956 startingPage "61" @default.
• W193644956 abstract "Genetic analysis of single cells is emerging as a powerful approach for studies of heterogeneous cell populations. Indeed, the notion of homogeneous cell populations is receding as approaches to resolve genetic and phenotypic variation between single cells are applied throughout the life sciences. A key step in single-cell genomic analysis today is the physical isolation of individual cells from heterogeneous populations, particularly microbial populations, which often exhibit high diversity. Here, we detail the construction and use of instrumentation for optical trapping inside microfluidic devices to select individual cells for analysis by methods including nucleic acid sequencing. This approach has unique advantages for analyses of rare community members, cells with irregular morphologies, small quantity samples, and studies that employ advanced optical microscopy." @default.
• W193644956 created "2016-06-24" @default.
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• W193644956 date "2013-01-01" @default.
• W193644956 modified "2023-09-27" @default.
• W193644956 title "Optofluidic Cell Selection from Complex Microbial Communities for Single-Genome Analysis" @default.
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• W193644956 doi "https://doi.org/10.1016/b978-0-12-407863-5.00004-6" @default.
• W193644956 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3886641" @default.
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