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- W1936697861 abstract "CEC is a high performance electrodriving liquid phase separation technique. It does not need complex and sophisticated high pressure instrumentation for nanoflow driving. This is attractive for parallel multicolumn analysis. To this end, high throughput methods for column preparation are needed to support the use of multiple columns. In this study, we directly used CEC mobile phase solution as the packing solvent, and realized rapid preparation of capillary columns based on a single particle fritting technology. The method presented high preparation throughput compared with other reported methods based on various fritting technologies. The single particle fritting approach promoted column preparation throughput to 1 column/h, including all the fritting, packing and conditioning steps. The rapidly prepared columns showed consistently high efficiency of up to 150 000 plates per meter, and usefulness in reversed phase CEC of neutral, charged and biomolecules. With standard peptides as the sample, excellent long term reproducibility (better than 0.8%RSD, ten days, for retention times) was observed." @default.
- W1936697861 created "2016-06-24" @default.
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- W1936697861 date "2013-12-27" @default.
- W1936697861 modified "2023-10-16" @default.
- W1936697861 title "Toward rapid preparation of capillary columns for electrochromatography use" @default.
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- W1936697861 doi "https://doi.org/10.1002/elps.201300503" @default.
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