Matches in SemOpenAlex for { <https://semopenalex.org/work/W1937264098> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1937264098 endingPage "330" @default.
- W1937264098 startingPage "325" @default.
- W1937264098 abstract "Encephalomyocarditis (EMC) viral RNA was isolated from purified virus grown in Ehrlich ascites tumor cells. The viral RNA was found to contain polyadenylic acid [poly(A)] regions that were very heterogeneous in length. Chromatography of the EMC viral RNA on oligo(dT)-cellulose columns separated the RNA into three distinct fractions (peaks 1 to 3). Approximately 20% of the EMC viral RNA appeared as peak 1, 40% as peak 2, and 40% as peak 3. The RNA in each fraction appeared to be intact as shown by co-sedimentation with 35S unfractionated EMC viral RNA in SDS-sucrose density gradients. Approximately 95 to 100% of peaks 1 and 3, and 60 to 70% of peak 2, reappeared at the same elution position after rechromatography on oligo(dT)-cellulose. The RNA in peak 1 contained poly(A) with an average length of 16 nucleotides, peak 2 contained poly(A) with an average of 26 nucleotides, and peak 3 contained an average of 74 nucleotides in its poly(A) region. The distribution in the three fractions, as well as the average length of the poly(A) moieties, was relatively unaffected by changes in the cell suspension medium used during infection. Finally, each of the three viral RNA fractions was assayed for biological activity using an infectious RNA assay on L-cell monolayers. Infectivity of the viral RNA was found to increase with poly(A) length, with peak 3 viral RNA being approximately 10 times more infectious than peak 1 viral RNA." @default.
- W1937264098 created "2016-06-24" @default.
- W1937264098 creator A5044496126 @default.
- W1937264098 creator A5082551727 @default.
- W1937264098 date "1976-08-01" @default.
- W1937264098 modified "2023-10-17" @default.
- W1937264098 title "Encephalomyocarditis virus RNA: variations in polyadenylic acid content and biological activity" @default.
- W1937264098 cites W112665875 @default.
- W1937264098 cites W1488634636 @default.
- W1937264098 cites W1560560788 @default.
- W1937264098 cites W1647375475 @default.
- W1937264098 cites W1858677515 @default.
- W1937264098 cites W1973295278 @default.
- W1937264098 cites W1977208035 @default.
- W1937264098 cites W1979000868 @default.
- W1937264098 cites W1979203581 @default.
- W1937264098 cites W1980260148 @default.
- W1937264098 cites W1989881459 @default.
- W1937264098 cites W1995775189 @default.
- W1937264098 cites W1999977989 @default.
- W1937264098 cites W2066160869 @default.
- W1937264098 cites W2066184324 @default.
- W1937264098 cites W2085061521 @default.
- W1937264098 cites W2092885340 @default.
- W1937264098 cites W2098884362 @default.
- W1937264098 cites W2126336050 @default.
- W1937264098 cites W2138013809 @default.
- W1937264098 cites W2145098108 @default.
- W1937264098 cites W2257644630 @default.
- W1937264098 doi "https://doi.org/10.1128/jvi.19.2.325-330.1976" @default.
- W1937264098 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/354869" @default.
- W1937264098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/183008" @default.
- W1937264098 hasPublicationYear "1976" @default.
- W1937264098 type Work @default.
- W1937264098 sameAs 1937264098 @default.
- W1937264098 citedByCount "47" @default.
- W1937264098 countsByYear W19372640982012 @default.
- W1937264098 countsByYear W19372640982018 @default.
- W1937264098 crossrefType "journal-article" @default.
- W1937264098 hasAuthorship W1937264098A5044496126 @default.
- W1937264098 hasAuthorship W1937264098A5082551727 @default.
- W1937264098 hasBestOaLocation W19372640981 @default.
- W1937264098 hasConcept C104317684 @default.
- W1937264098 hasConcept C106358424 @default.
- W1937264098 hasConcept C108190873 @default.
- W1937264098 hasConcept C153911025 @default.
- W1937264098 hasConcept C159047783 @default.
- W1937264098 hasConcept C2522874641 @default.
- W1937264098 hasConcept C41258723 @default.
- W1937264098 hasConcept C512185932 @default.
- W1937264098 hasConcept C55493867 @default.
- W1937264098 hasConcept C67705224 @default.
- W1937264098 hasConcept C86803240 @default.
- W1937264098 hasConceptScore W1937264098C104317684 @default.
- W1937264098 hasConceptScore W1937264098C106358424 @default.
- W1937264098 hasConceptScore W1937264098C108190873 @default.
- W1937264098 hasConceptScore W1937264098C153911025 @default.
- W1937264098 hasConceptScore W1937264098C159047783 @default.
- W1937264098 hasConceptScore W1937264098C2522874641 @default.
- W1937264098 hasConceptScore W1937264098C41258723 @default.
- W1937264098 hasConceptScore W1937264098C512185932 @default.
- W1937264098 hasConceptScore W1937264098C55493867 @default.
- W1937264098 hasConceptScore W1937264098C67705224 @default.
- W1937264098 hasConceptScore W1937264098C86803240 @default.
- W1937264098 hasIssue "2" @default.
- W1937264098 hasLocation W19372640981 @default.
- W1937264098 hasLocation W19372640982 @default.
- W1937264098 hasLocation W19372640983 @default.
- W1937264098 hasLocation W19372640984 @default.
- W1937264098 hasOpenAccess W1937264098 @default.
- W1937264098 hasPrimaryLocation W19372640981 @default.
- W1937264098 hasRelatedWork W1487857142 @default.
- W1937264098 hasRelatedWork W1953426278 @default.
- W1937264098 hasRelatedWork W1986137505 @default.
- W1937264098 hasRelatedWork W2020475514 @default.
- W1937264098 hasRelatedWork W2027511035 @default.
- W1937264098 hasRelatedWork W2072505260 @default.
- W1937264098 hasRelatedWork W2076100628 @default.
- W1937264098 hasRelatedWork W2088644387 @default.
- W1937264098 hasRelatedWork W2139087689 @default.
- W1937264098 hasRelatedWork W2169137691 @default.
- W1937264098 hasVolume "19" @default.
- W1937264098 isParatext "false" @default.
- W1937264098 isRetracted "false" @default.
- W1937264098 magId "1937264098" @default.
- W1937264098 workType "article" @default.