FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1937647947> ?p ?o ?g. }

• W1937647947 endingPage "1560.e3" @default.
• W1937647947 startingPage "1552" @default.
• W1937647947 abstract "ObjectiveTo investigate the engraftment and proliferation of superparamagnetic iron oxide nanoparticles (SPIOs)-labeled human CD133+ bone marrow-derived stem cells (BMDSCs) in an animal model of Asherman syndrome (AS).DesignProspective experimental animal study.SettingUniversity research laboratories.Animal(s)Nonobese diabetic mice (strain code 394; NOD.CB17- Prkdcscid/NcrCrl) in which AS was induced according to a published protocol.Intervention(s)Human CD133+ BMDSCs were obtained from patients undergoing autologous cell therapy in refractory AS and endometrial atrophy, labeled with SPIOs and injected either intrauterinely (n = 5) or systemically through the tail vein (n = 5) in the animal model.Main Outcome Measure(s)Accumulation of collagen and glycosaminoglycan deposits detected by trichrome staining. Percentage and localization of engrafted human SPIOs-labeled CD133+ BMDSCs by Prussian blue staining. Cell proliferation assay using Ki67 and reverse transcriptase–polymerase chain reaction (PCR) for specific paracrine factors.Result(s)The induction of the AS in the murine model was demonstrated by the accumulation of collagen and glycosaminoglycan deposits in the damaged horns by trichrome staining. Human SPIOs labeled CD133+ BMDSCs homing represents 0.59% and 0.65% of total number of cells present in the horns after intrauterine or tail vein injections, respectively. Engrafted cells were localized around endometrial blood vessels, inducing proliferation in surrounding cells based on Ki67 and regulation of the paracrine factors thrombospondin 1 and insulin-like growth factor 1.Conclusion(s)The injection of human SPIOs labeled CD133+ BMDSCs in a murine model of AS confirms that these cells engraft around endometrial vessels, inducing proliferation of surrounding cells through paracrine molecules such as thrombospondin 1 and insulin-like growth factor 1.Clinical Trial Registration NumberNCT02144987. To investigate the engraftment and proliferation of superparamagnetic iron oxide nanoparticles (SPIOs)-labeled human CD133+ bone marrow-derived stem cells (BMDSCs) in an animal model of Asherman syndrome (AS). Prospective experimental animal study. University research laboratories. Nonobese diabetic mice (strain code 394; NOD.CB17- Prkdcscid/NcrCrl) in which AS was induced according to a published protocol. Human CD133+ BMDSCs were obtained from patients undergoing autologous cell therapy in refractory AS and endometrial atrophy, labeled with SPIOs and injected either intrauterinely (n = 5) or systemically through the tail vein (n = 5) in the animal model. Accumulation of collagen and glycosaminoglycan deposits detected by trichrome staining. Percentage and localization of engrafted human SPIOs-labeled CD133+ BMDSCs by Prussian blue staining. Cell proliferation assay using Ki67 and reverse transcriptase–polymerase chain reaction (PCR) for specific paracrine factors. The induction of the AS in the murine model was demonstrated by the accumulation of collagen and glycosaminoglycan deposits in the damaged horns by trichrome staining. Human SPIOs labeled CD133+ BMDSCs homing represents 0.59% and 0.65% of total number of cells present in the horns after intrauterine or tail vein injections, respectively. Engrafted cells were localized around endometrial blood vessels, inducing proliferation in surrounding cells based on Ki67 and regulation of the paracrine factors thrombospondin 1 and insulin-like growth factor 1. The injection of human SPIOs labeled CD133+ BMDSCs in a murine model of AS confirms that these cells engraft around endometrial vessels, inducing proliferation of surrounding cells through paracrine molecules such as thrombospondin 1 and insulin-like growth factor 1." @default.
• W1937647947 created "2016-06-24" @default.
• W1937647947 creator A5002141076 @default.
• W1937647947 creator A5014138630 @default.
• W1937647947 creator A5027057730 @default.
• W1937647947 creator A5028328854 @default.
• W1937647947 creator A5035502229 @default.
• W1937647947 creator A5037102206 @default.
• W1937647947 creator A5069717224 @default.
• W1937647947 creator A5090345459 @default.
• W1937647947 date "2015-12-01" @default.
• W1937647947 modified "2023-10-17" @default.
• W1937647947 title "Human CD133+ bone marrow-derived stem cells promote endometrial proliferation in a murine model of Asherman syndrome" @default.
• W1937647947 cites W1502896205 @default.
• W1937647947 cites W1964554602 @default.
• W1937647947 cites W1964847244 @default.
• W1937647947 cites W1965425745 @default.
• W1937647947 cites W1967954462 @default.
• W1937647947 cites W1977872287 @default.
• W1937647947 cites W1977876562 @default.
• W1937647947 cites W1978458314 @default.
• W1937647947 cites W1979103623 @default.
• W1937647947 cites W1979767149 @default.
• W1937647947 cites W1987207032 @default.
• W1937647947 cites W1996890527 @default.
• W1937647947 cites W2010545057 @default.
• W1937647947 cites W2010899501 @default.
• W1937647947 cites W2026490398 @default.
• W1937647947 cites W2028490033 @default.
• W1937647947 cites W2031885111 @default.
• W1937647947 cites W2035183737 @default.
• W1937647947 cites W2037085506 @default.
• W1937647947 cites W2038777331 @default.
• W1937647947 cites W2038916787 @default.
• W1937647947 cites W2042598375 @default.
• W1937647947 cites W2048412962 @default.
• W1937647947 cites W2050266242 @default.
• W1937647947 cites W2054794301 @default.
• W1937647947 cites W2063341926 @default.
• W1937647947 cites W2064441872 @default.
• W1937647947 cites W2073608758 @default.
• W1937647947 cites W2090409413 @default.
• W1937647947 cites W2107888949 @default.
• W1937647947 cites W2112711017 @default.
• W1937647947 cites W2115701515 @default.
• W1937647947 cites W2124396531 @default.
• W1937647947 cites W2124496335 @default.
• W1937647947 cites W2127313908 @default.
• W1937647947 cites W2128491618 @default.
• W1937647947 cites W2130794824 @default.
• W1937647947 cites W2132512472 @default.
• W1937647947 cites W2133691598 @default.
• W1937647947 cites W2143516059 @default.
• W1937647947 cites W2146710021 @default.
• W1937647947 cites W2146815044 @default.
• W1937647947 cites W2157869969 @default.
• W1937647947 cites W4310693891 @default.
• W1937647947 doi "https://doi.org/10.1016/j.fertnstert.2015.08.032" @default.
• W1937647947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26384164" @default.
• W1937647947 hasPublicationYear "2015" @default.
• W1937647947 type Work @default.
• W1937647947 sameAs 1937647947 @default.
• W1937647947 citedByCount "110" @default.
• W1937647947 countsByYear W19376479472016 @default.
• W1937647947 countsByYear W19376479472017 @default.
• W1937647947 countsByYear W19376479472018 @default.
• W1937647947 countsByYear W19376479472019 @default.
• W1937647947 countsByYear W19376479472020 @default.
• W1937647947 countsByYear W19376479472021 @default.
• W1937647947 countsByYear W19376479472022 @default.
• W1937647947 countsByYear W19376479472023 @default.
• W1937647947 crossrefType "journal-article" @default.
• W1937647947 hasAuthorship W1937647947A5002141076 @default.
• W1937647947 hasAuthorship W1937647947A5014138630 @default.
• W1937647947 hasAuthorship W1937647947A5027057730 @default.
• W1937647947 hasAuthorship W1937647947A5028328854 @default.
• W1937647947 hasAuthorship W1937647947A5035502229 @default.
• W1937647947 hasAuthorship W1937647947A5037102206 @default.
• W1937647947 hasAuthorship W1937647947A5069717224 @default.
• W1937647947 hasAuthorship W1937647947A5090345459 @default.
• W1937647947 hasBestOaLocation W19376479471 @default.
• W1937647947 hasConcept C125473707 @default.
• W1937647947 hasConcept C126322002 @default.
• W1937647947 hasConcept C142724271 @default.
• W1937647947 hasConcept C163031210 @default.
• W1937647947 hasConcept C170493617 @default.
• W1937647947 hasConcept C28328180 @default.
• W1937647947 hasConcept C59469219 @default.
• W1937647947 hasConcept C71924100 @default.
• W1937647947 hasConcept C74864618 @default.
• W1937647947 hasConcept C7876069 @default.
• W1937647947 hasConcept C86803240 @default.
• W1937647947 hasConcept C95444343 @default.
• W1937647947 hasConceptScore W1937647947C125473707 @default.
• W1937647947 hasConceptScore W1937647947C126322002 @default.
• W1937647947 hasConceptScore W1937647947C142724271 @default.
• W1937647947 hasConceptScore W1937647947C163031210 @default.
• W1937647947 hasConceptScore W1937647947C170493617 @default.

• next