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- W1937989488 abstract "The major challenge of the post‐genome world is ascribing in situ function to the myriad of proteins expressed in the proteome. This challenge is met by an arsenal of inactivation strategies that include RNAi and genetic knockout. These are powerful approaches but are indirect with respect to protein function and are subject to time delays before onset and possible genetic compensation. This chapter describes two protein‐based inactivation approaches called chromophore‐assisted laser inactivation (CALI) and fluorophore‐assisted light inactivation (FALI). For CALI and FALI, light inactivation is targeted via photosensitizers that are localized to proteins of interest through antibody binding or expressed domains that are fluorescent or bind fluorescent probes. Inactivation occurs when and where the cells or tissues are irradiated and thus CALI and FALI provide an unprecedented level of spatial and temporal resolution of protein inactivation. Here we provide methods for the labeling of antibodies and setup of light sources and discuss controls, advantages of the technology, and potential pitfalls. We conclude with a discussion on a number of new technologies derived from CALI that combine molecular genetic approaches with light‐induced inactivation that provide new tools to address in situ protein function." @default.
- W1937989488 created "2016-06-24" @default.
- W1937989488 creator A5017883303 @default.
- W1937989488 creator A5050465015 @default.
- W1937989488 creator A5067308734 @default.
- W1937989488 creator A5068119691 @default.
- W1937989488 date "2007-01-01" @default.
- W1937989488 modified "2023-10-18" @default.
- W1937989488 title "Chromophore‐Assisted Laser Inactivation" @default.
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- W1937989488 doi "https://doi.org/10.1016/s0091-679x(06)82011-x" @default.
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