Matches in SemOpenAlex for { <https://semopenalex.org/work/W1938711844> ?p ?o ?g. }
- W1938711844 endingPage "6326" @default.
- W1938711844 startingPage "6319" @default.
- W1938711844 abstract "Integration host factor (IHF) is a DNA-binding and -bending protein that has been found in a number of gram-negative bacteria. Here we describe the cloning, sequencing, and functional analysis of the genes coding for the two subunits of IHF from Pseudomonas putida. Both the ihfA and ihfB genes of P. putida code for 100-amino-acid-residue polypeptides that are 1 and 6 residues longer than the Escherichia coli IHF subunits, respectively. The P. putida ihfA and ihfB genes can effectively complement E. coli ihf mutants, suggesting that the P. putida IHF subunits can form functional heterodimers with the IHF subunits of E. coli. Analysis of the amino acid differences between the E. coli and P. putida protein sequences suggests that in the evolution of IHF, amino acid changes were mainly restricted to the N-terminal domains and to the extreme C termini. These changes do not interfere with dimer formation or with DNA recognition. We constructed a P. putida mutant strain carrying an ihfA gene knockout and demonstrated that IHF is essential for the expression of the P(U) promoter of the xyl operon of the upper pathway of toluene degradation. It was further shown that the ihfA P. putida mutant strain carrying the TOL plasmid was defective in the degradation of the aromatic model compound benzyl alcohol, proving the unique role of IHF in xyl operon promoter regulation." @default.
- W1938711844 created "2016-06-24" @default.
- W1938711844 creator A5025962702 @default.
- W1938711844 creator A5025975328 @default.
- W1938711844 creator A5027892876 @default.
- W1938711844 creator A5031618127 @default.
- W1938711844 creator A5037766176 @default.
- W1938711844 creator A5039062818 @default.
- W1938711844 creator A5041573681 @default.
- W1938711844 creator A5042926209 @default.
- W1938711844 creator A5066612048 @default.
- W1938711844 creator A5087635774 @default.
- W1938711844 creator A5088582483 @default.
- W1938711844 date "1996-11-01" @default.
- W1938711844 modified "2023-09-26" @default.
- W1938711844 title "Structure and function of the Pseudomonas putida integration host factor" @default.
- W1938711844 cites W1017438797 @default.
- W1938711844 cites W1487135478 @default.
- W1938711844 cites W1569511038 @default.
- W1938711844 cites W1572009433 @default.
- W1938711844 cites W1580221530 @default.
- W1938711844 cites W1694207595 @default.
- W1938711844 cites W1940781171 @default.
- W1938711844 cites W1964713595 @default.
- W1938711844 cites W1973310140 @default.
- W1938711844 cites W1977254684 @default.
- W1938711844 cites W1984798625 @default.
- W1938711844 cites W1985642742 @default.
- W1938711844 cites W1988119525 @default.
- W1938711844 cites W2000275924 @default.
- W1938711844 cites W2000288069 @default.
- W1938711844 cites W2004360551 @default.
- W1938711844 cites W2009088204 @default.
- W1938711844 cites W2010422998 @default.
- W1938711844 cites W2011093920 @default.
- W1938711844 cites W2031546361 @default.
- W1938711844 cites W2032750490 @default.
- W1938711844 cites W2039877146 @default.
- W1938711844 cites W2040154659 @default.
- W1938711844 cites W2045991415 @default.
- W1938711844 cites W2049306412 @default.
- W1938711844 cites W2050406602 @default.
- W1938711844 cites W2064449445 @default.
- W1938711844 cites W2074122548 @default.
- W1938711844 cites W2082927796 @default.
- W1938711844 cites W2086174686 @default.
- W1938711844 cites W2090631246 @default.
- W1938711844 cites W2106551108 @default.
- W1938711844 cites W2117859683 @default.
- W1938711844 cites W2153581415 @default.
- W1938711844 cites W294776928 @default.
- W1938711844 cites W31306344 @default.
- W1938711844 cites W69571828 @default.
- W1938711844 doi "https://doi.org/10.1128/jb.178.21.6319-6326.1996" @default.
- W1938711844 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/178507" @default.
- W1938711844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8892836" @default.
- W1938711844 hasPublicationYear "1996" @default.
- W1938711844 type Work @default.
- W1938711844 sameAs 1938711844 @default.
- W1938711844 citedByCount "34" @default.
- W1938711844 countsByYear W19387118442012 @default.
- W1938711844 countsByYear W19387118442013 @default.
- W1938711844 countsByYear W19387118442014 @default.
- W1938711844 countsByYear W19387118442015 @default.
- W1938711844 countsByYear W19387118442016 @default.
- W1938711844 countsByYear W19387118442021 @default.
- W1938711844 crossrefType "journal-article" @default.
- W1938711844 hasAuthorship W1938711844A5025962702 @default.
- W1938711844 hasAuthorship W1938711844A5025975328 @default.
- W1938711844 hasAuthorship W1938711844A5027892876 @default.
- W1938711844 hasAuthorship W1938711844A5031618127 @default.
- W1938711844 hasAuthorship W1938711844A5037766176 @default.
- W1938711844 hasAuthorship W1938711844A5039062818 @default.
- W1938711844 hasAuthorship W1938711844A5041573681 @default.
- W1938711844 hasAuthorship W1938711844A5042926209 @default.
- W1938711844 hasAuthorship W1938711844A5066612048 @default.
- W1938711844 hasAuthorship W1938711844A5087635774 @default.
- W1938711844 hasAuthorship W1938711844A5088582483 @default.
- W1938711844 hasBestOaLocation W19387118442 @default.
- W1938711844 hasConcept C101762097 @default.
- W1938711844 hasConcept C104317684 @default.
- W1938711844 hasConcept C143065580 @default.
- W1938711844 hasConcept C150194340 @default.
- W1938711844 hasConcept C153911025 @default.
- W1938711844 hasConcept C158448853 @default.
- W1938711844 hasConcept C167625842 @default.
- W1938711844 hasConcept C203075996 @default.
- W1938711844 hasConcept C22744801 @default.
- W1938711844 hasConcept C2779606329 @default.
- W1938711844 hasConcept C2780543182 @default.
- W1938711844 hasConcept C515207424 @default.
- W1938711844 hasConcept C547475151 @default.
- W1938711844 hasConcept C5527404 @default.
- W1938711844 hasConcept C55493867 @default.
- W1938711844 hasConcept C86803240 @default.
- W1938711844 hasConceptScore W1938711844C101762097 @default.
- W1938711844 hasConceptScore W1938711844C104317684 @default.
- W1938711844 hasConceptScore W1938711844C143065580 @default.