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- W1940398494 abstract "Summary The possibility of separating ascites tumor cells with different numbers of chromosomes by means of counter-streaming centrifugation has been studied with the hyperdiploid Ehrlich ascites tumor (ELD) and its hypertetraploid offshoot (ELT). From ELD a fraction significantly higher in deoxyribonucleic acid phosphorus (DNAP) per cell than the unfractionated suspension was obtained. Inoculated into a new mouse this fraction developed into a suspension which was invariably lower in DNAP per cell than the inoculum. Repeated inoculation of this fraction followed by further fractionation increased the mean number of chromosomes per cell, giving about twice the original number. When another fraction of ELD, which is low in DNAP, was inoculated, the tumor suspension produced showed the same value of DNAP as that of the inoculum. Fractionation of original ELT gave two fractions high in DNAP per cell, one composed chiefly of octoploid cells, and another of tetraploid and octoploid cells. The distribution of the different types of tumor cells upon the three fractions obtained with counter-streaming centrifugation is not in keeping with that which should be expected from their volumes and contents of DNA." @default.
- W1940398494 created "2016-06-24" @default.
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- W1940398494 date "1960-07-01" @default.
- W1940398494 modified "2023-09-23" @default.
- W1940398494 title "Separation of ascites tumor cells rich in deoxyribonucleic acid by means of counter-streaming centrifugation." @default.
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