FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1940694714> ?p ?o ?g. }

• W1940694714 endingPage "6172" @default.
• W1940694714 startingPage "6165" @default.
• W1940694714 abstract "Assembly and maturation of retroviral particles requires the aggregation and controlled proteolytic cleavage of polyprotein core precursors by a precursor-encoded protease (PR). Active, mature retroviral PR is a dimer, and the accumulation of precursors at sites of assembly may facilitate subunit interaction and subsequent activation of this enzyme. In addition, it has been suggested that cellular cytoplasmic components act as inhibitors of PR activity, so that processing is delayed until the nascent virions leave this compartment and separate from the surface of host cells. To investigate the mechanisms that control PR activity during virus assembly, we studied the in vivo processing of retroviral gag precursors that contain tandemly linked PR subunits in which dimerization is concentration independent. Sequences encoding four different linked protease dimers were independently joined to the end of the Rous sarcoma virus (RSV) gag gene in a simian virus 40-based plasmid vector which expresses a myristoylated gag precursor upon transfection of COS-1 cells. Three of these plasmids produced gag precursors that were incorporated into viruslike particles and proteolytically cleaved by the dimers to mature core proteins that were indistinguishable from the processed products of wild-type gag. The amount of viral gag protein that was assembled and packaged in these transfections was inversely related to the relative proteolytic activities of the linked PR dimers. The fourth gag precursor, which contained the most active linked PR dimer, underwent rapid intracellular processing and did not form viruslike particles. In the absence of the plasma membrane targeting signal, processing of all four linked PR dimer-containing gag precursors was completed entirely within the cell. From these results, we conclude that the delay in polyprotein core precursor processing that occurs during normal virion assembly does not depend on a cytoplasmic inhibitor of PR activity. We suggest that dimer formation is not only necessary but may be sufficient for the initiation of PR-directed maturation of gag and gag-pol precursors." @default.
• W1940694714 created "2016-06-24" @default.
• W1940694714 creator A5046088041 @default.
• W1940694714 creator A5062135663 @default.
• W1940694714 creator A5084801786 @default.
• W1940694714 date "1991-11-01" @default.
• W1940694714 modified "2023-10-11" @default.
• W1940694714 title "Assembly and processing of avian retroviral gag polyproteins containing linked protease dimers" @default.
• W1940694714 cites W1509948560 @default.
• W1940694714 cites W1554599785 @default.
• W1940694714 cites W1564616498 @default.
• W1940694714 cites W1575815180 @default.
• W1940694714 cites W1591869598 @default.
• W1940694714 cites W1604779495 @default.
• W1940694714 cites W1609065924 @default.
• W1940694714 cites W1623171066 @default.
• W1940694714 cites W1753916721 @default.
• W1940694714 cites W1754066784 @default.
• W1940694714 cites W1895846194 @default.
• W1940694714 cites W1896629372 @default.
• W1940694714 cites W1908085580 @default.
• W1940694714 cites W1914738526 @default.
• W1940694714 cites W1949162842 @default.
• W1940694714 cites W1966398224 @default.
• W1940694714 cites W1985604746 @default.
• W1940694714 cites W1986248827 @default.
• W1940694714 cites W1989475707 @default.
• W1940694714 cites W1994564305 @default.
• W1940694714 cites W1995396220 @default.
• W1940694714 cites W1997369616 @default.
• W1940694714 cites W2014027374 @default.
• W1940694714 cites W2033553403 @default.
• W1940694714 cites W2040779333 @default.
• W1940694714 cites W2052919376 @default.
• W1940694714 cites W2054898895 @default.
• W1940694714 cites W2056405362 @default.
• W1940694714 cites W2061205020 @default.
• W1940694714 cites W2068066098 @default.
• W1940694714 cites W2089227678 @default.
• W1940694714 cites W2115869555 @default.
• W1940694714 cites W2146946460 @default.
• W1940694714 cites W2160660298 @default.
• W1940694714 cites W2165732545 @default.
• W1940694714 doi "https://doi.org/10.1128/jvi.65.11.6165-6172.1991" @default.
• W1940694714 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/250303" @default.
• W1940694714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1656090" @default.
• W1940694714 hasPublicationYear "1991" @default.
• W1940694714 type Work @default.
• W1940694714 sameAs 1940694714 @default.
• W1940694714 citedByCount "43" @default.
• W1940694714 countsByYear W19406947142013 @default.
• W1940694714 countsByYear W19406947142015 @default.
• W1940694714 countsByYear W19406947142017 @default.
• W1940694714 crossrefType "journal-article" @default.
• W1940694714 hasAuthorship W1940694714A5046088041 @default.
• W1940694714 hasAuthorship W1940694714A5062135663 @default.
• W1940694714 hasAuthorship W1940694714A5084801786 @default.
• W1940694714 hasBestOaLocation W19406947141 @default.
• W1940694714 hasConcept C104317684 @default.
• W1940694714 hasConcept C11960822 @default.
• W1940694714 hasConcept C153911025 @default.
• W1940694714 hasConcept C159047783 @default.
• W1940694714 hasConcept C181199279 @default.
• W1940694714 hasConcept C190062978 @default.
• W1940694714 hasConcept C2522874641 @default.
• W1940694714 hasConcept C2776714187 @default.
• W1940694714 hasConcept C2777441520 @default.
• W1940694714 hasConcept C2778431562 @default.
• W1940694714 hasConcept C2779562191 @default.
• W1940694714 hasConcept C2910895815 @default.
• W1940694714 hasConcept C54009773 @default.
• W1940694714 hasConcept C55493867 @default.
• W1940694714 hasConcept C6881719 @default.
• W1940694714 hasConcept C77207865 @default.
• W1940694714 hasConcept C86803240 @default.
• W1940694714 hasConcept C95444343 @default.
• W1940694714 hasConceptScore W1940694714C104317684 @default.
• W1940694714 hasConceptScore W1940694714C11960822 @default.
• W1940694714 hasConceptScore W1940694714C153911025 @default.
• W1940694714 hasConceptScore W1940694714C159047783 @default.
• W1940694714 hasConceptScore W1940694714C181199279 @default.
• W1940694714 hasConceptScore W1940694714C190062978 @default.
• W1940694714 hasConceptScore W1940694714C2522874641 @default.
• W1940694714 hasConceptScore W1940694714C2776714187 @default.
• W1940694714 hasConceptScore W1940694714C2777441520 @default.
• W1940694714 hasConceptScore W1940694714C2778431562 @default.
• W1940694714 hasConceptScore W1940694714C2779562191 @default.
• W1940694714 hasConceptScore W1940694714C2910895815 @default.
• W1940694714 hasConceptScore W1940694714C54009773 @default.
• W1940694714 hasConceptScore W1940694714C55493867 @default.
• W1940694714 hasConceptScore W1940694714C6881719 @default.
• W1940694714 hasConceptScore W1940694714C77207865 @default.
• W1940694714 hasConceptScore W1940694714C86803240 @default.
• W1940694714 hasConceptScore W1940694714C95444343 @default.
• W1940694714 hasIssue "11" @default.
• W1940694714 hasLocation W19406947141 @default.
• W1940694714 hasLocation W19406947142 @default.
• W1940694714 hasLocation W19406947143 @default.

• next