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- W194103637 abstract "CD4(+)CD25(+)FOXP3(+) T regulatory (Treg) cells are pivotal for the induction and maintenance of peripheral tolerance in both mice and humans. The possibility to use Treg cells for the treatment of T-cell-mediated diseases has recently gained increasing momentum. However, given the limited amount of circulating FOXP3(+) Treg cells, efficient methods for their ex vivo expansion are highly desirable. Rapamycin allows for in vitro expansion of murine and human FOXP3(+) Treg cells, which maintain their regulatory phenotype and suppressive capacity. Here, we describe in detail the powerful methods for enriching human FOXP3(+) Treg cells starting from unfractionated CD4(+) T cells or for expanding CD25(+)-enriched Treg cells in the presence of rapamycin." @default.
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- W194103637 date "2011-09-29" @default.
- W194103637 modified "2023-10-09" @default.
- W194103637 title "Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin" @default.
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- W194103637 doi "https://doi.org/10.1007/978-1-61779-430-8_17" @default.
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