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• W1944166069 abstract "ABSTRACT The addition of α-amanitin to extrafollicular, cumulus-enclosed ovine oocytes at explantation inhibits meiotic maturation and prevents many of the changes in protein synthesis that normally accompany maturation. By contrast, these inhibitory effects are considerably reduced by either delaying the addition of the drug for 1-4 h or by denuding the oocytes of all associated cumulus cells at the onset of culture. The observations that the inhibitory effect of cordycepin on nuclear maturation is also time-dependent and cumulus-cell-dependent and that the oocyte is susceptible to cordycepin for longer than its sensitivity to α-amanitin are consistent with the differential effects of these drugs on RNA synthesis. It is concluded that a transcriptional event at the onset of maturation is essential for the initiation of those changes in protein synthesis required for the regulation of nuclear and cytoplasmic maturation. It is uncertain, however, whether this transcriptional event occurs within the cumulus cells or within the oocyte." @default.
• W1944166069 created "2016-06-24" @default.
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• W1944166069 date "1983-02-01" @default.
• W1944166069 modified "2023-09-23" @default.
• W1944166069 title "Time-dependent effects of α-amanitin on nuclear maturation and protein synthesis in mammalian oocytes" @default.
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• W1944166069 doi "https://doi.org/10.1242/dev.73.1.317" @default.
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