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• W1944258462 abstract "Fenugreek ( Trigonella foenum-graecum L.) plant is cultivated throughout the world as  medicinal, food, condiment, dye, and forage. First biotechnological study was carried out in 1945 that reported the effects of diniconazole, a triazole-type fungicide on cell suspension cultures of fenugreek. It was followed by many studies using cell suspension and callus culture emphasising increased production of protein and economically important metabolites like trigonelline, sapogenin, Isoflavonoid pterocarpans, diosgenin, gitogenin and tigogenin from callus, leaves, stems and roots  explants. Plant tissue culture studies have emphasied use of callus, cotyloden, hypocotyls and  shoot tip epicotyls, apical meristem, cotyledon node and cotyledon leaf explants. Most of the researchers agree difficulty in in vitro rooting. Protoplast studies have also been reported  using leaf mesophyll and  root apices. Leaf mesophyl protoplasts could be converted to leafy shoots whereas, root apices protoplasts gave cell colonies or roots only. Genetic transformation studies using Agrobacterium rhizogenes and A. tumefaciens are at initial stages and the genes used in the  studies only present  transformation with either marker or reporter genes. There is a single report on molecular charachterisation of fenugreek from India using 10 RAPD and  ISSR primers that revealed interspecific polymorphysm. A review of all this suggests that plant biotechnology of fenugreek is at initial stages of development and there is lot to do for the improvement and breeding of fenugreek." @default.
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• W1944258462 date "2014-03-05" @default.
• W1944258462 modified "2023-09-26" @default.
• W1944258462 title "Current Trends In Fenugreek Bıotechnology And Approaches Towards Its Improvement" @default.
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