FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1944450188> ?p ?o ?g. }

• W1944450188 endingPage "e1002192" @default.
• W1944450188 startingPage "e1002192" @default.
• W1944450188 abstract "To provide tight spatiotemporal signaling control, the cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) holoenzyme typically nucleates a macromolecular complex or a PKA signalosome. Using the RIIβ holoenzyme as a prototype, we show how autophosphorylation/dephosphorylation of the RIIβ subunit, as well as cAMP and metal ions, contribute to the dynamics of PKA signaling. While we showed previously that the RIIβ holoenzyme could undergo a single turnover autophosphorylation with adenosine triphosphate and magnesium (MgATP) and trap both products in the crystal lattice, we asked here whether calcium could trap an ATP:RIIβ holoenzyme since the RIIβ holoenzyme is located close to ion channels. The 2.8Å structure of an RIIβp2:C2:(Ca2ADP)2 holoenzyme, supported by biochemical and biophysical data, reveals a trapped single phosphorylation event similar to MgATP. Thus, calcium can mediate a single turnover event with either ATP or adenosine-5'-(β,γ-imido)triphosphate (AMP-PNP), even though it cannot support steady-state catalysis efficiently. The holoenzyme serves as a product trap because of the slow off-rate of the pRIIβ subunit, which is controlled by cAMP, not by phosphorylation of the inhibitor site. By quantitatively defining the RIIβ signaling cycle, we show that release of pRIIβ in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site (P-site) inhibits holoenzyme reassociation with the catalytic subunit. Adding a single phosphoryl group to the preformed RIIβ holoenzyme thus creates a signaling cycle in which phosphatases become an essential partner. This previously unappreciated molecular mechanism is an integral part of PKA signaling for type II holoenzymes." @default.
• W1944450188 created "2016-06-24" @default.
• W1944450188 creator A5006568947 @default.
• W1944450188 creator A5045962448 @default.
• W1944450188 creator A5048203630 @default.
• W1944450188 creator A5055549206 @default.
• W1944450188 creator A5059314538 @default.
• W1944450188 creator A5059580925 @default.
• W1944450188 creator A5075228460 @default.
• W1944450188 creator A5081112969 @default.
• W1944450188 date "2015-07-09" @default.
• W1944450188 modified "2023-10-18" @default.
• W1944450188 title "Single Turnover Autophosphorylation Cycle of the PKA RIIβ Holoenzyme" @default.
• W1944450188 cites W1490238718 @default.
• W1944450188 cites W1521291324 @default.
• W1944450188 cites W1592938589 @default.
• W1944450188 cites W1954353020 @default.
• W1944450188 cites W1968150128 @default.
• W1944450188 cites W1970174659 @default.
• W1944450188 cites W1977730145 @default.
• W1944450188 cites W1987437644 @default.
• W1944450188 cites W1992325160 @default.
• W1944450188 cites W2001137280 @default.
• W1944450188 cites W2002117545 @default.
• W1944450188 cites W2007346017 @default.
• W1944450188 cites W2027282106 @default.
• W1944450188 cites W2033630329 @default.
• W1944450188 cites W2036184042 @default.
• W1944450188 cites W2037572737 @default.
• W1944450188 cites W2055439983 @default.
• W1944450188 cites W2061505650 @default.
• W1944450188 cites W2061684132 @default.
• W1944450188 cites W2067405360 @default.
• W1944450188 cites W2072398950 @default.
• W1944450188 cites W2076018512 @default.
• W1944450188 cites W2076777258 @default.
• W1944450188 cites W2091455173 @default.
• W1944450188 cites W2100400861 @default.
• W1944450188 cites W2104424507 @default.
• W1944450188 cites W2111912667 @default.
• W1944450188 cites W2112720988 @default.
• W1944450188 cites W2117326207 @default.
• W1944450188 cites W2126920991 @default.
• W1944450188 cites W2156339041 @default.
• W1944450188 cites W2156682932 @default.
• W1944450188 cites W2953375613 @default.
• W1944450188 cites W617003878 @default.
• W1944450188 cites W2132251464 @default.
• W1944450188 doi "https://doi.org/10.1371/journal.pbio.1002192" @default.
• W1944450188 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4497662" @default.
• W1944450188 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26158466" @default.
• W1944450188 hasPublicationYear "2015" @default.
• W1944450188 type Work @default.
• W1944450188 sameAs 1944450188 @default.
• W1944450188 citedByCount "29" @default.
• W1944450188 countsByYear W19444501882015 @default.
• W1944450188 countsByYear W19444501882016 @default.
• W1944450188 countsByYear W19444501882017 @default.
• W1944450188 countsByYear W19444501882018 @default.
• W1944450188 countsByYear W19444501882019 @default.
• W1944450188 countsByYear W19444501882020 @default.
• W1944450188 countsByYear W19444501882021 @default.
• W1944450188 countsByYear W19444501882022 @default.
• W1944450188 crossrefType "journal-article" @default.
• W1944450188 hasAuthorship W1944450188A5006568947 @default.
• W1944450188 hasAuthorship W1944450188A5045962448 @default.
• W1944450188 hasAuthorship W1944450188A5048203630 @default.
• W1944450188 hasAuthorship W1944450188A5055549206 @default.
• W1944450188 hasAuthorship W1944450188A5059314538 @default.
• W1944450188 hasAuthorship W1944450188A5059580925 @default.
• W1944450188 hasAuthorship W1944450188A5075228460 @default.
• W1944450188 hasAuthorship W1944450188A5081112969 @default.
• W1944450188 hasBestOaLocation W19444501881 @default.
• W1944450188 hasConcept C104292427 @default.
• W1944450188 hasConcept C104317684 @default.
• W1944450188 hasConcept C11960822 @default.
• W1944450188 hasConcept C12554922 @default.
• W1944450188 hasConcept C150109051 @default.
• W1944450188 hasConcept C178666793 @default.
• W1944450188 hasConcept C181912034 @default.
• W1944450188 hasConcept C2779564974 @default.
• W1944450188 hasConcept C55493867 @default.
• W1944450188 hasConcept C86803240 @default.
• W1944450188 hasConcept C95444343 @default.
• W1944450188 hasConcept C97029542 @default.
• W1944450188 hasConceptScore W1944450188C104292427 @default.
• W1944450188 hasConceptScore W1944450188C104317684 @default.
• W1944450188 hasConceptScore W1944450188C11960822 @default.
• W1944450188 hasConceptScore W1944450188C12554922 @default.
• W1944450188 hasConceptScore W1944450188C150109051 @default.
• W1944450188 hasConceptScore W1944450188C178666793 @default.
• W1944450188 hasConceptScore W1944450188C181912034 @default.
• W1944450188 hasConceptScore W1944450188C2779564974 @default.
• W1944450188 hasConceptScore W1944450188C55493867 @default.
• W1944450188 hasConceptScore W1944450188C86803240 @default.
• W1944450188 hasConceptScore W1944450188C95444343 @default.
• W1944450188 hasConceptScore W1944450188C97029542 @default.
• W1944450188 hasIssue "7" @default.

• next