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- W1944821569 abstract "ABSTRACT We have measured UV-induced mutation frequencies in yeast in a forward, nonselective assay system by scoring white adex ade2 double auxotrophs among parental red-pigmented ade2 clones. The frequencies of sectored and pure mutant clones were determined separately. In excision-defective strains carrying the genes radl-1, rad3-2 and rad4-4, as well as in the double mutants, rad 1-1 rad 3-2 and rad 1-1 rad 4-4, considerably more sectored than pure clones are induced in the low-dose range; in repair-competent strains, pure mutant clones substantially outnumber the sectored clones. These results can be explained on the basis of known differences in the timing of error-prone repair during the cell division cycle; that is, we assume that error-prone repair occurs primarily before replication in RAD wild-type strains but after replication in excision-deficient mutants. It has been suggested that excision deficiency has a pleiotropic effect on heteroduplex repair and nucleotide excision repair; however, the high percentage (36.6%) of half-sectored clones found in the rad1-1 strain is hard to reconcile with this hypothesis. We propose that heteroduplex repair occurs subsequent to error-prone repair in both excisionproficient and excision-deficient strains." @default.
- W1944821569 created "2016-06-24" @default.
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- W1944821569 date "1980-05-01" @default.
- W1944821569 modified "2023-10-01" @default.
- W1944821569 title "HETERODUPLEX REPAIR AS AN INTERMEDIATE STEP OF UV MUTAGENESIS IN YEAST" @default.
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- W1944821569 doi "https://doi.org/10.1093/genetics/95.1.63" @default.
- W1944821569 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1214222" @default.
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