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- W1944993609 abstract "Capillary zone electrophoresis (CZE) has emerged as a novel technique for the rapid and effective separation of serum proteins (1)(2)(3)(4)(5)(6)(7)(8). Recently, a multichannel automated system for CZE of human serum proteins (Paragon 2000 clinical capillary electrophoresis system; Beckman Instruments) became commercially available; this system offers a clinically reliable alternative to cellulose acetate and agarose electrophoresis. CZE has the advantage of automation, improved precision, and a faster turnaround time (6)(8).In the conventional methods, quantification of the protein fractions is based on dye binding, whereas CZE uses ultraviolet detection at 214 nm for direct protein quantification via the peptide bonds. We asked whether intravascular agents such as radio-contrast media that absorb at 214 nm would simulate a monoclonal component on CZE.We performed high resolution agarose electrophoresis with the Hydrasys analyzer (Sebia), using Hydragel 15 HR gels (Sebia) according to the …" @default.
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- W1944993609 date "1999-01-01" @default.
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- W1944993609 title "Interference of Radio-opaque Agents in Clinical Capillary Zone Electrophoresis" @default.
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- W1944993609 doi "https://doi.org/10.1093/clinchem/45.1.129" @default.
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