Matches in SemOpenAlex for { <https://semopenalex.org/work/W1945291271> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1945291271 endingPage "10" @default.
- W1945291271 startingPage "7" @default.
- W1945291271 abstract "Sixty-four kinds of cell lines were examined as to their ability to degrade glucagon using conditioned-media obtained from their protein-free cultures. Two human tumor cell lines were shown to produce this activity, and the cell line, HPC-YO, established from a human pancreatic carcinoma was shown to produce the highest level of activity. The glucagon-degrading enzyme (GDE) was purified from HPC-YO conditioned-medium by a combination of ion-exchange, gel filtration, and hydroxylapatite column chromatographies. The purified GDE also degraded vasoactive intestinal polypeptide (VIP) and secretin, however, it did not cleave EGF, gastrin, insulin, somatostatin, substance P, neurotensin, or growth hormone. The molecular weight of GDE is 83,000, as determined on SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of GDE was blocked, and the five partial amino acid sequences obtained on lysyl-endopeptidase digestion were determined to be N-L-T-E-E-Y-D-V-S-D-G-E-I-E-L-L-Y-E-K, V-E-T-Y-Y-D-L-L-F-E-K, L-Y-W-F-L-D-E-A-K, S-N-S-T-S-Y-V-K, and Y-Y-A-S-T-S-Y-D-D-T-Y-K. The same or homologous amino acid sequences have not been found in known proteins, demonstrating that GDE is a novel peptidase that degrades the secretin family: glucagon, VIP, and secretin." @default.
- W1945291271 created "2016-06-24" @default.
- W1945291271 creator A5006160249 @default.
- W1945291271 creator A5012332712 @default.
- W1945291271 creator A5028059547 @default.
- W1945291271 creator A5037253804 @default.
- W1945291271 creator A5040930407 @default.
- W1945291271 creator A5049596064 @default.
- W1945291271 creator A5060693017 @default.
- W1945291271 creator A5083496309 @default.
- W1945291271 creator A5090133184 @default.
- W1945291271 creator A5090750728 @default.
- W1945291271 date "1995-01-01" @default.
- W1945291271 modified "2023-09-24" @default.
- W1945291271 title "A Novel Proteinase, Glucagon-Degrading Enzyme, Secreted by a Human Pancreatic Cancer Cell Line, HPC-YO" @default.
- W1945291271 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a124724" @default.
- W1945291271 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7775401" @default.
- W1945291271 hasPublicationYear "1995" @default.
- W1945291271 type Work @default.
- W1945291271 sameAs 1945291271 @default.
- W1945291271 citedByCount "3" @default.
- W1945291271 countsByYear W19452912712012 @default.
- W1945291271 crossrefType "journal-article" @default.
- W1945291271 hasAuthorship W1945291271A5006160249 @default.
- W1945291271 hasAuthorship W1945291271A5012332712 @default.
- W1945291271 hasAuthorship W1945291271A5028059547 @default.
- W1945291271 hasAuthorship W1945291271A5037253804 @default.
- W1945291271 hasAuthorship W1945291271A5040930407 @default.
- W1945291271 hasAuthorship W1945291271A5049596064 @default.
- W1945291271 hasAuthorship W1945291271A5060693017 @default.
- W1945291271 hasAuthorship W1945291271A5083496309 @default.
- W1945291271 hasAuthorship W1945291271A5090133184 @default.
- W1945291271 hasAuthorship W1945291271A5090750728 @default.
- W1945291271 hasConcept C118303440 @default.
- W1945291271 hasConcept C153911025 @default.
- W1945291271 hasConcept C170493617 @default.
- W1945291271 hasConcept C181199279 @default.
- W1945291271 hasConcept C185592680 @default.
- W1945291271 hasConcept C2777774944 @default.
- W1945291271 hasConcept C2778563252 @default.
- W1945291271 hasConcept C2778677841 @default.
- W1945291271 hasConcept C2778764654 @default.
- W1945291271 hasConcept C515207424 @default.
- W1945291271 hasConcept C54355233 @default.
- W1945291271 hasConcept C55493867 @default.
- W1945291271 hasConcept C71315377 @default.
- W1945291271 hasConcept C81885089 @default.
- W1945291271 hasConcept C8330315 @default.
- W1945291271 hasConcept C86803240 @default.
- W1945291271 hasConceptScore W1945291271C118303440 @default.
- W1945291271 hasConceptScore W1945291271C153911025 @default.
- W1945291271 hasConceptScore W1945291271C170493617 @default.
- W1945291271 hasConceptScore W1945291271C181199279 @default.
- W1945291271 hasConceptScore W1945291271C185592680 @default.
- W1945291271 hasConceptScore W1945291271C2777774944 @default.
- W1945291271 hasConceptScore W1945291271C2778563252 @default.
- W1945291271 hasConceptScore W1945291271C2778677841 @default.
- W1945291271 hasConceptScore W1945291271C2778764654 @default.
- W1945291271 hasConceptScore W1945291271C515207424 @default.
- W1945291271 hasConceptScore W1945291271C54355233 @default.
- W1945291271 hasConceptScore W1945291271C55493867 @default.
- W1945291271 hasConceptScore W1945291271C71315377 @default.
- W1945291271 hasConceptScore W1945291271C81885089 @default.
- W1945291271 hasConceptScore W1945291271C8330315 @default.
- W1945291271 hasConceptScore W1945291271C86803240 @default.
- W1945291271 hasIssue "1" @default.
- W1945291271 hasLocation W19452912711 @default.
- W1945291271 hasLocation W19452912712 @default.
- W1945291271 hasOpenAccess W1945291271 @default.
- W1945291271 hasPrimaryLocation W19452912711 @default.
- W1945291271 hasRelatedWork W1735667659 @default.
- W1945291271 hasRelatedWork W1945291271 @default.
- W1945291271 hasRelatedWork W2010964553 @default.
- W1945291271 hasRelatedWork W2072539021 @default.
- W1945291271 hasRelatedWork W2095034273 @default.
- W1945291271 hasRelatedWork W2401363067 @default.
- W1945291271 hasRelatedWork W2405554635 @default.
- W1945291271 hasRelatedWork W2411370480 @default.
- W1945291271 hasRelatedWork W2424946052 @default.
- W1945291271 hasRelatedWork W6616317 @default.
- W1945291271 hasVolume "117" @default.
- W1945291271 isParatext "false" @default.
- W1945291271 isRetracted "false" @default.
- W1945291271 magId "1945291271" @default.
- W1945291271 workType "article" @default.