Matches in SemOpenAlex for { <https://semopenalex.org/work/W1945379875> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1945379875 startingPage "3858" @default.
- W1945379875 abstract "Introduction Recent studies proved mitochondrial DNA fragments (mtDNA) were released to the extracellular space and closely involved in modulating native immune response. However, the role of extracellular mtDNA in pathogenesis of COPD is unclearObjective We measured circulating mtDNA level and pro-inflammatory factors in COPD patients and investigate the role of its specific receptor toll-like receptor 9 (TLR9)Methods 122 COPD patients and 79 controls were recruited. mtDNA copies(specific mitochondrial gene ND1,CYTB,COXIII)in patients serum and supernatants of airway epithelium cells were determined using qRT-PCR.TNF-a,IL-8,CRPwere assayed by multiplexing ELISA. Further investigation on TLR9 in CSE induced cell signaling activation was performed at RNA and protein level using antagonist.Results In COPD patients, serum mtDNA increased significantly with increasing severity. mtDNA level in smokers was 1.3 fold higher compared to non-smokers(ND1copies 5.9±1.3x10^4Vs4.6±1.1x10^4,p<0.05).There is an elevation of IL-8 level in patients compared to controls(4.86±2.26Vs6.14±3.12ng/ml,p<0.05). Correlation analysis showed serum mtDNA COXIII was positively correlated with IL-8 (r=0.375,p=0.033).Abundant mtDNA was found to release extracellularly from 16HBE and H292 when induced by CSE. The expression of TLR9 significantly increased in a dose and time-dependent manner and blockage of TLR9 inhibited CSE induced MAPK signaling pathway activationConclusions : Elevation of circulating mtDNA level is found prevalent in patients with increasing COPD severity and positively correlated with IL-8. Extracellular mtDNA may play an essential role in modulating airway inflammation in COPD." @default.
- W1945379875 created "2016-06-24" @default.
- W1945379875 creator A5014464917 @default.
- W1945379875 creator A5021032631 @default.
- W1945379875 creator A5041175709 @default.
- W1945379875 creator A5055059649 @default.
- W1945379875 creator A5064621040 @default.
- W1945379875 creator A5085142676 @default.
- W1945379875 date "2014-09-01" @default.
- W1945379875 modified "2023-09-27" @default.
- W1945379875 title "The role of circulating mitochondrial DNA in modulating airway inflammation of chronic obstructive pulmonary disease" @default.
- W1945379875 hasPublicationYear "2014" @default.
- W1945379875 type Work @default.
- W1945379875 sameAs 1945379875 @default.
- W1945379875 citedByCount "0" @default.
- W1945379875 crossrefType "journal-article" @default.
- W1945379875 hasAuthorship W1945379875A5014464917 @default.
- W1945379875 hasAuthorship W1945379875A5021032631 @default.
- W1945379875 hasAuthorship W1945379875A5041175709 @default.
- W1945379875 hasAuthorship W1945379875A5055059649 @default.
- W1945379875 hasAuthorship W1945379875A5064621040 @default.
- W1945379875 hasAuthorship W1945379875A5085142676 @default.
- W1945379875 hasConcept C104317684 @default.
- W1945379875 hasConcept C126322002 @default.
- W1945379875 hasConcept C150194340 @default.
- W1945379875 hasConcept C153911025 @default.
- W1945379875 hasConcept C170493617 @default.
- W1945379875 hasConcept C190727270 @default.
- W1945379875 hasConcept C203014093 @default.
- W1945379875 hasConcept C24586158 @default.
- W1945379875 hasConcept C2776780178 @default.
- W1945379875 hasConcept C2776914184 @default.
- W1945379875 hasConcept C2779138994 @default.
- W1945379875 hasConcept C2780942790 @default.
- W1945379875 hasConcept C28406088 @default.
- W1945379875 hasConcept C28859421 @default.
- W1945379875 hasConcept C54355233 @default.
- W1945379875 hasConcept C71924100 @default.
- W1945379875 hasConcept C86803240 @default.
- W1945379875 hasConcept C95444343 @default.
- W1945379875 hasConceptScore W1945379875C104317684 @default.
- W1945379875 hasConceptScore W1945379875C126322002 @default.
- W1945379875 hasConceptScore W1945379875C150194340 @default.
- W1945379875 hasConceptScore W1945379875C153911025 @default.
- W1945379875 hasConceptScore W1945379875C170493617 @default.
- W1945379875 hasConceptScore W1945379875C190727270 @default.
- W1945379875 hasConceptScore W1945379875C203014093 @default.
- W1945379875 hasConceptScore W1945379875C24586158 @default.
- W1945379875 hasConceptScore W1945379875C2776780178 @default.
- W1945379875 hasConceptScore W1945379875C2776914184 @default.
- W1945379875 hasConceptScore W1945379875C2779138994 @default.
- W1945379875 hasConceptScore W1945379875C2780942790 @default.
- W1945379875 hasConceptScore W1945379875C28406088 @default.
- W1945379875 hasConceptScore W1945379875C28859421 @default.
- W1945379875 hasConceptScore W1945379875C54355233 @default.
- W1945379875 hasConceptScore W1945379875C71924100 @default.
- W1945379875 hasConceptScore W1945379875C86803240 @default.
- W1945379875 hasConceptScore W1945379875C95444343 @default.
- W1945379875 hasLocation W19453798751 @default.
- W1945379875 hasOpenAccess W1945379875 @default.
- W1945379875 hasPrimaryLocation W19453798751 @default.
- W1945379875 hasRelatedWork W1998785280 @default.
- W1945379875 hasRelatedWork W2040945977 @default.
- W1945379875 hasRelatedWork W2111316413 @default.
- W1945379875 hasRelatedWork W2143127936 @default.
- W1945379875 hasRelatedWork W2163601934 @default.
- W1945379875 hasRelatedWork W2543068196 @default.
- W1945379875 hasRelatedWork W2590701429 @default.
- W1945379875 hasRelatedWork W2745829652 @default.
- W1945379875 hasRelatedWork W2859606301 @default.
- W1945379875 hasRelatedWork W2897545321 @default.
- W1945379875 hasRelatedWork W2899400632 @default.
- W1945379875 hasRelatedWork W2913066936 @default.
- W1945379875 hasRelatedWork W2916260118 @default.
- W1945379875 hasRelatedWork W3009368225 @default.
- W1945379875 hasRelatedWork W3013707936 @default.
- W1945379875 hasRelatedWork W3039749606 @default.
- W1945379875 hasRelatedWork W3100939609 @default.
- W1945379875 hasRelatedWork W3136104852 @default.
- W1945379875 hasRelatedWork W3172586800 @default.
- W1945379875 hasRelatedWork W3207662319 @default.
- W1945379875 hasVolume "44" @default.
- W1945379875 isParatext "false" @default.
- W1945379875 isRetracted "false" @default.
- W1945379875 magId "1945379875" @default.
- W1945379875 workType "article" @default.