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- W194606443 abstract "In the present experiments minute quantities (10(-4), 10(-5), 10(-6) TCID(50)) of adenovirus type 12were used for the infection of culture cells, HeLa-S. After infecting the cells with virus, the tissue cultures were conducted by the slant agar and monolayer methods, to compare the degree of virus proliferation for successive generations in each experimental group. The results of the study are summarized as follows. 1. When HeLa S(3) cells were infected with minute quantities of virus for successive generations (30 days each generation), the virus proliferation, as determined by cytopathic effect (CPE), was better in the case of the slant agar culture than in the monolayer culture. 2. Similar cultures were conducted by two methods in which the culture period was changed to 7 days or 30-35 days for each generation. As a result it was found that the proliferation of the virus could be detected only at the fifth or sixth generation in all the experimental groups. Both the slant agar culture and the monolayer culture of infected HeLa-S(3) cells gave the results similar to those obtained in the successive cultures conducted by the freezingthawing method (reported in Part 1)." @default.
- W194606443 created "2016-06-24" @default.
- W194606443 creator A5068391520 @default.
- W194606443 date "1968-08-30" @default.
- W194606443 modified "2023-09-24" @default.
- W194606443 title "Adenovirus 12型に関する研究 第II編 Adenovirus 12型の寒天斜面組織培養法及び単層組織培養法による増殖について" @default.
- W194606443 hasPublicationYear "1968" @default.
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