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- W194617009 abstract "Publisher Summary This chapter introduces a rapid genetic method for the study of ribonucleic acid (RNA) binding proteins (RNA-BP). This method is useful for: (1) identifying mutations in the RNA or the RNA-BP that affect binding affinity and/or specificity, (2) obtaining quantitative estimates of relative binding affinities from repression ratios easily measured in E. coli , and (3) developing models for RNA–protein interaction on the basis of the altered specificity or enhanced affinity of RNA-BP suppressor mutants thereby, identified and characterized. Using this approach, translational repression of modified lacZ transcripts is achieved not only with Rev, but also with other heterologous RNA-BPs including: U1A, nucleolin, and alfalfa mosaic virus coat protein. A future goal is to adapt this method for cloning complementary deoxyribonucleic acids (cDNAs) encoding RNA-BPs that bind to specific RNA sequences. This chapter discusses about the quantitative model for translation repression by heterologous RNA-BP S . The chapter also explains concepts related to methods such as strains, plasmids, and RNA-BP plasmid. Preparation and use of competent cells is also discussed. In addition, the chapter highlights the conditions for expression of the RNA-BP in E.coli and elaborates upon estimating dissociation constants." @default.
- W194617009 created "2016-06-24" @default.
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- W194617009 date "1997-01-01" @default.
- W194617009 modified "2023-09-28" @default.
- W194617009 title "A Rapid Genetic Method for the Study of RNA Binding Proteins" @default.
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- W194617009 doi "https://doi.org/10.1016/b978-012587545-5/50015-x" @default.
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