Matches in SemOpenAlex for { <https://semopenalex.org/work/W1946391270> ?p ?o ?g. }
- W1946391270 endingPage "5914" @default.
- W1946391270 startingPage "5907" @default.
- W1946391270 abstract "The genes for the alpha and beta subunits of the enzyme protocatechuate 3,4-dioxygenase (EC 1.13.11.3) were cloned from the Pseudomonas cepacia DBO1 chromosome on a 9.5-kilobase-pair PstI fragment into the broad-host-range cloning vector pRO2317. The resultant clone was able to complement protocatechuate 3,4-dioxugenase mutations in P. cepacia, Pseudomonas aeruginosa, and Pseudomonas putida. Expression studies showed that the genes were constitutively expressed and subject to catabolite repression in the heterologous host. Since the cloned genes exhibited normal induction patterns when present in P. cepacia DBO1, it was concluded that induction was subject to negative control. Regulatory studies with P. cepacia wild-type and mutant strains showed that protocatechuate 3,4-dioxygenase is induced either by protocatechuate or by beta-carboxymuconate. Further studies of P. cepacia DBO1 showed that p-hydroxybenzoate hydroxylase (EC 1.14.13.2), the preceding enzyme in the pathway, is induced by p-hydroxybenzoate and that beta-carboxymuconate lactonizing enzyme, which catalyzes the reaction following protocatechuate 3,4-dioxygenase, is induced by both p-hydroxybenzoate and beta-ketoadipate." @default.
- W1946391270 created "2016-06-24" @default.
- W1946391270 creator A5004252995 @default.
- W1946391270 creator A5006324851 @default.
- W1946391270 creator A5023125790 @default.
- W1946391270 date "1989-11-01" @default.
- W1946391270 modified "2023-10-12" @default.
- W1946391270 title "Cloning, expression, and regulation of the Pseudomonas cepacia protocatechuate 3,4-dioxygenase genes" @default.
- W1946391270 cites W1413884192 @default.
- W1946391270 cites W1480682589 @default.
- W1946391270 cites W1482048712 @default.
- W1946391270 cites W1482265991 @default.
- W1946391270 cites W1486946238 @default.
- W1946391270 cites W1491417004 @default.
- W1946391270 cites W1492160446 @default.
- W1946391270 cites W1494980574 @default.
- W1946391270 cites W1501205262 @default.
- W1946391270 cites W1517580958 @default.
- W1946391270 cites W1524688180 @default.
- W1946391270 cites W1553239339 @default.
- W1946391270 cites W1573137827 @default.
- W1946391270 cites W1581681869 @default.
- W1946391270 cites W1588380766 @default.
- W1946391270 cites W1626426536 @default.
- W1946391270 cites W1662167897 @default.
- W1946391270 cites W1709345302 @default.
- W1946391270 cites W173253527 @default.
- W1946391270 cites W1762668287 @default.
- W1946391270 cites W1834101661 @default.
- W1946391270 cites W1896499155 @default.
- W1946391270 cites W1947871521 @default.
- W1946391270 cites W1949766905 @default.
- W1946391270 cites W197854464 @default.
- W1946391270 cites W1979588233 @default.
- W1946391270 cites W1987334529 @default.
- W1946391270 cites W2011108549 @default.
- W1946391270 cites W2019410656 @default.
- W1946391270 cites W2031453432 @default.
- W1946391270 cites W2049635421 @default.
- W1946391270 cites W2051163396 @default.
- W1946391270 cites W2058741248 @default.
- W1946391270 cites W2061202321 @default.
- W1946391270 cites W2067347565 @default.
- W1946391270 cites W2092632656 @default.
- W1946391270 cites W2110088230 @default.
- W1946391270 cites W2113339074 @default.
- W1946391270 cites W2114213729 @default.
- W1946391270 cites W2128635872 @default.
- W1946391270 cites W2130992353 @default.
- W1946391270 cites W2156402236 @default.
- W1946391270 cites W2179103414 @default.
- W1946391270 cites W2338592893 @default.
- W1946391270 cites W2950103804 @default.
- W1946391270 cites W3164596334 @default.
- W1946391270 cites W4293247451 @default.
- W1946391270 cites W51481324 @default.
- W1946391270 doi "https://doi.org/10.1128/jb.171.11.5907-5914.1989" @default.
- W1946391270 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/210453" @default.
- W1946391270 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2808302" @default.
- W1946391270 hasPublicationYear "1989" @default.
- W1946391270 type Work @default.
- W1946391270 sameAs 1946391270 @default.
- W1946391270 citedByCount "72" @default.
- W1946391270 countsByYear W19463912702013 @default.
- W1946391270 countsByYear W19463912702014 @default.
- W1946391270 countsByYear W19463912702015 @default.
- W1946391270 countsByYear W19463912702016 @default.
- W1946391270 countsByYear W19463912702021 @default.
- W1946391270 countsByYear W19463912702023 @default.
- W1946391270 crossrefType "journal-article" @default.
- W1946391270 hasAuthorship W1946391270A5004252995 @default.
- W1946391270 hasAuthorship W1946391270A5006324851 @default.
- W1946391270 hasAuthorship W1946391270A5023125790 @default.
- W1946391270 hasBestOaLocation W19463912701 @default.
- W1946391270 hasConcept C104317684 @default.
- W1946391270 hasConcept C143065580 @default.
- W1946391270 hasConcept C150194340 @default.
- W1946391270 hasConcept C153911025 @default.
- W1946391270 hasConcept C168374021 @default.
- W1946391270 hasConcept C19924922 @default.
- W1946391270 hasConcept C2776738503 @default.
- W1946391270 hasConcept C2776834422 @default.
- W1946391270 hasConcept C2780543182 @default.
- W1946391270 hasConcept C2781463711 @default.
- W1946391270 hasConcept C523546767 @default.
- W1946391270 hasConcept C54355233 @default.
- W1946391270 hasConcept C55493867 @default.
- W1946391270 hasConcept C83941689 @default.
- W1946391270 hasConcept C86803240 @default.
- W1946391270 hasConcept C89423630 @default.
- W1946391270 hasConceptScore W1946391270C104317684 @default.
- W1946391270 hasConceptScore W1946391270C143065580 @default.
- W1946391270 hasConceptScore W1946391270C150194340 @default.
- W1946391270 hasConceptScore W1946391270C153911025 @default.
- W1946391270 hasConceptScore W1946391270C168374021 @default.
- W1946391270 hasConceptScore W1946391270C19924922 @default.
- W1946391270 hasConceptScore W1946391270C2776738503 @default.
- W1946391270 hasConceptScore W1946391270C2776834422 @default.