Matches in SemOpenAlex for { <https://semopenalex.org/work/W1946556756> ?p ?o ?g. }
- W1946556756 endingPage "997" @default.
- W1946556756 startingPage "992" @default.
- W1946556756 abstract "Summary The novel mutant streptokinase, SK-K59E, can activate human plasminogen as efficiently as the purified commercially available streptokinase. Several peptide bonds including Lys59-Ser60 in native streptokinase were hydrolyzed in reaction with plasmin and peptides of small molecular masses were generated. The plasminogen activator activity of native streptokinase in reaction with human plasmin declined to 25% of the original activity in a 120-min incubation. On the other hand, the NH2-terminal peptide of SK-K59E remained intact in reaction with plasmin and the activator activity of streptokinase decreased to 75% of the original activity in 120 min. The major degraded peptide fragments of native streptokinase in reaction with plasmin had molecular masses of 36 and 30 kDa. However, two major peptide fragments of 42 and 34 kDa were observed in the reaction of SK-K59E with human plasmin. The 42 kDa peptide fragment, which contained NH2-terminal of streptokinase, could activate human plasminogen as efficiently as the native streptokinase. SK-K59E can induce greater degree of caseinolysis and fibrinolysis than the native streptokinase. In conclusion, the results demonstrate that the prevention of cleavage at Lys59 of streptokinase prolongs the half-life of streptokinase in complex with plasmin and that the NH2-terminal of streptokinase (Ile1-Lys59) plays an important role in maintaining its stability." @default.
- W1946556756 created "2016-06-24" @default.
- W1946556756 creator A5025004801 @default.
- W1946556756 creator A5038932176 @default.
- W1946556756 creator A5046959339 @default.
- W1946556756 creator A5048858926 @default.
- W1946556756 creator A5060090208 @default.
- W1946556756 creator A5062499106 @default.
- W1946556756 creator A5068229730 @default.
- W1946556756 creator A5084393015 @default.
- W1946556756 date "1998-01-01" @default.
- W1946556756 modified "2023-10-17" @default.
- W1946556756 title "Preparation of a Novel Streptokinase Mutant with Improved Stability" @default.
- W1946556756 cites W1497163285 @default.
- W1946556756 cites W1590159040 @default.
- W1946556756 cites W1597599446 @default.
- W1946556756 cites W1637599331 @default.
- W1946556756 cites W167177991 @default.
- W1946556756 cites W1699728700 @default.
- W1946556756 cites W1804472714 @default.
- W1946556756 cites W1966087742 @default.
- W1946556756 cites W1981765089 @default.
- W1946556756 cites W1990020735 @default.
- W1946556756 cites W1993908496 @default.
- W1946556756 cites W2012839797 @default.
- W1946556756 cites W2027418031 @default.
- W1946556756 cites W2043473501 @default.
- W1946556756 cites W2062957366 @default.
- W1946556756 cites W2076195491 @default.
- W1946556756 cites W2084500047 @default.
- W1946556756 cites W2100837269 @default.
- W1946556756 cites W2101108802 @default.
- W1946556756 cites W2138270253 @default.
- W1946556756 doi "https://doi.org/10.1055/s-0037-1615108" @default.
- W1946556756 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9609235" @default.
- W1946556756 hasPublicationYear "1998" @default.
- W1946556756 type Work @default.
- W1946556756 sameAs 1946556756 @default.
- W1946556756 citedByCount "15" @default.
- W1946556756 countsByYear W19465567562013 @default.
- W1946556756 countsByYear W19465567562016 @default.
- W1946556756 countsByYear W19465567562017 @default.
- W1946556756 countsByYear W19465567562018 @default.
- W1946556756 countsByYear W19465567562019 @default.
- W1946556756 countsByYear W19465567562020 @default.
- W1946556756 crossrefType "journal-article" @default.
- W1946556756 hasAuthorship W1946556756A5025004801 @default.
- W1946556756 hasAuthorship W1946556756A5038932176 @default.
- W1946556756 hasAuthorship W1946556756A5046959339 @default.
- W1946556756 hasAuthorship W1946556756A5048858926 @default.
- W1946556756 hasAuthorship W1946556756A5060090208 @default.
- W1946556756 hasAuthorship W1946556756A5062499106 @default.
- W1946556756 hasAuthorship W1946556756A5068229730 @default.
- W1946556756 hasAuthorship W1946556756A5084393015 @default.
- W1946556756 hasConcept C126322002 @default.
- W1946556756 hasConcept C134018914 @default.
- W1946556756 hasConcept C153911025 @default.
- W1946556756 hasConcept C170493617 @default.
- W1946556756 hasConcept C181199279 @default.
- W1946556756 hasConcept C185592680 @default.
- W1946556756 hasConcept C2776336681 @default.
- W1946556756 hasConcept C2776825266 @default.
- W1946556756 hasConcept C2779281246 @default.
- W1946556756 hasConcept C2779679481 @default.
- W1946556756 hasConcept C2781071845 @default.
- W1946556756 hasConcept C500558357 @default.
- W1946556756 hasConcept C55493867 @default.
- W1946556756 hasConcept C71924100 @default.
- W1946556756 hasConcept C86803240 @default.
- W1946556756 hasConcept C88045685 @default.
- W1946556756 hasConceptScore W1946556756C126322002 @default.
- W1946556756 hasConceptScore W1946556756C134018914 @default.
- W1946556756 hasConceptScore W1946556756C153911025 @default.
- W1946556756 hasConceptScore W1946556756C170493617 @default.
- W1946556756 hasConceptScore W1946556756C181199279 @default.
- W1946556756 hasConceptScore W1946556756C185592680 @default.
- W1946556756 hasConceptScore W1946556756C2776336681 @default.
- W1946556756 hasConceptScore W1946556756C2776825266 @default.
- W1946556756 hasConceptScore W1946556756C2779281246 @default.
- W1946556756 hasConceptScore W1946556756C2779679481 @default.
- W1946556756 hasConceptScore W1946556756C2781071845 @default.
- W1946556756 hasConceptScore W1946556756C500558357 @default.
- W1946556756 hasConceptScore W1946556756C55493867 @default.
- W1946556756 hasConceptScore W1946556756C71924100 @default.
- W1946556756 hasConceptScore W1946556756C86803240 @default.
- W1946556756 hasConceptScore W1946556756C88045685 @default.
- W1946556756 hasIssue "05" @default.
- W1946556756 hasLocation W19465567561 @default.
- W1946556756 hasLocation W19465567562 @default.
- W1946556756 hasOpenAccess W1946556756 @default.
- W1946556756 hasPrimaryLocation W19465567561 @default.
- W1946556756 hasRelatedWork W1946556756 @default.
- W1946556756 hasRelatedWork W2015240976 @default.
- W1946556756 hasRelatedWork W2018534042 @default.
- W1946556756 hasRelatedWork W2023594738 @default.
- W1946556756 hasRelatedWork W2395157942 @default.
- W1946556756 hasRelatedWork W2409335028 @default.
- W1946556756 hasRelatedWork W2410164848 @default.