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• W1947261073 abstract "Three temperature-sensitive (ts) mutants of simian virus 40 (SV40) in complementation group A (tsA7, tsA28, tsA30) have been isolated and characterized in permissive and restrictive host cells. At 41 C in the AH line of African green monkey kidney cells, the mutants are deficient in an early function required to produce infectious viral deoxyribonucleic acid (DNA). Temperature-shift experiments and analysis of SV40 viral DNA replication by gel electrophoresis have provided strong evidence that the ts gene product of the three mutants is directly required to initiate each new round of viral DNA replication but is not required to complete a cycle which has already begun. The synthesis of mutant DNA molecules themselves can be initiated by a nonmutant gene product in viral complementation studies at 41 C. The cell, however, cannot substitute a host function to provide the initiator required for the replication of free viral DNA. The viral initiator is also required to establish the stable transformation of 3T3 cells." @default.
• W1947261073 created "2016-06-24" @default.
• W1947261073 creator A5007304390 @default.
• W1947261073 date "1972-10-01" @default.
• W1947261073 modified "2023-10-12" @default.
• W1947261073 title "Simian Virus 40 Deoxyribonucleic Acid Synthesis: the Viral Replicon" @default.
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• W1947261073 doi "https://doi.org/10.1128/jvi.10.4.591-598.1972" @default.
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