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- W1947753982 abstract "Genome-wide association studies have suggested that, compared to non-O blood types, the ABO O blood type phenotype and related genotypes are associated with protection from CVD. To test the hypothesis that ABO glycosyltransferase modulates endothelial functional pathways and glycan profile, we differentiated adipose stem cell (ASC)-derived induced pluripotent stem cell (iPSC) from individuals of different ABO blood types to endothelial cells (ECs). iPSC-ECs exhibited cobble-stone morphology, demonstrated capillary tube formation on matrigel, and incorporated acetylated low-density lipoprotein. FACS analysis indicates expression of both CD31 and vascular endothelial growth factor receptor-2 (VEGFR2). Immunocytochemical analysis of iPSC-ECs revealed positive staining for EC markers e.g., CD31, vascular endothelial cadherin (CD144), and von Willebrand factor (vWF). The phenotypic characteristics of iPSC-ECs were similar to that of human coronary arterial endothelial cells (hCAECs). The adhesion pattern of iPSC-EC to a variety of extracellular matrix (ECM) was similar across O and non-O iPSC-EC, but ABO blood group O showed lesser adhesion to vitronectin and laminin (mean±SD; 2.37±0.19 folds in non-O vs. O for vitronectin [p=0.02] and 2.30±0.05 folds in non-O vs. O for laminin [p=0.004]). Further, gap closure assay showed that O type iPSC-EC had greater migration capacity. We examined glycan profiles of pooled iPSC-ECs and pooled hCAECs from subjects with matched blood types. N-linked glycan profiling showed that series of high-mannose structures were observed as main components of iPSC-EC and hCAECs. Complex structures were observed as minor components. iPSC-ECs which gave similar profile to that of hCAECs. Main O-glycans in iPSC-ECs and hCAECs was a monosialylated core 1 structure. In summary, we established the feasibility of differentiating EC from subject-specific ASC-iPSC as tools to define disease-relevant ABO modification of EC functions. Grant Funding Source: NIH U01, K24" @default.
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- W1947753982 date "2014-04-01" @default.
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- W1947753982 title "ABO modulation of endothelial phenotypes and glycome profile in human induced pluripotent stem cell‐derived endothelial cells (1003.2)" @default.
- W1947753982 doi "https://doi.org/10.1096/fasebj.28.1_supplement.1003.2" @default.
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