FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1947892387> ?p ?o ?g. }

• W1947892387 endingPage "3822" @default.
• W1947892387 startingPage "3813" @default.
• W1947892387 abstract "Cells of the monocyte/macrophage lineage are involved in the development of inflammatory joint diseases such as rheumatoid arthritis. This disease is characterized by cartilage degradation and synovial membrane inflammation with a progressive loss of joint function. The pathological processes are still not well understood. Therefore it would be interesting to develop a suitable experimental in vitro model system for defined studies of monocyte/macrophage and chondrocyte interactions at the molecular level. For that purpose we cocultured chondrocytes from adult human articular cartilage with human monocytes and macrophages for defined periods of time in agarose without addition of serum. We performed zymographic and western blot analysis of culture medium, completed by quantitative RT-PCR of each chondrocyte, monocyte and macrophage RNA, respectively. The reliability of the newly established coculture systems is confirmed by causing a clear decrease of intact aggrecan in the coculture medium plus concurrent appearance of additional smaller fragments and a reduction of chondrocyte aggrecan and collagen II gene expression in the presence of monocytes. In culture medium from cocultures we detected active forms of the matrix metalloproteinases MMP-1, MMP-3 and MMP-9 accompanied by induction of gene expression of MMP-1, membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in chondrocytes. No gene expression of MMP-9 was detectable in chondrocytes, the enzyme was solely expressed in monocytes and macrophages and was downregulated in the presence of chondrocytes. Our results suggest that MMP-9 protein in coculture medium originated from monocytes and macrophages but activation required chondrocyte-derived factors. Because addition of plasmin, a partial activator of pro-MMP-3 and pro-MMP-1, enhanced the activation of pro-MMP-9 and pro-MMP-1 in cocultures but not in monocultured macrophages, and the presence of MMP-3 inhibitor II prevented pro-MMP-9 activation, we assumed a stepwise activation process of pro-MMP-9 that is dependent on the presence of at least MMP-3 and possibly also MMP-1." @default.
• W1947892387 created "2016-06-24" @default.
• W1947892387 creator A5001954776 @default.
• W1947892387 creator A5003537474 @default.
• W1947892387 creator A5015148582 @default.
• W1947892387 creator A5023575597 @default.
• W1947892387 creator A5060926236 @default.
• W1947892387 date "2001-11-01" @default.
• W1947892387 modified "2023-10-05" @default.
• W1947892387 title "Paracrine interactions of chondrocytes and macrophages in cartilage degradation: articular chondrocytes provide factors that activate macrophage-derived pro-gelatinase B (pro-MMP-9)" @default.
• W1947892387 cites W1482195692 @default.
• W1947892387 cites W1649452503 @default.
• W1947892387 cites W193383588 @default.
• W1947892387 cites W1963946720 @default.
• W1947892387 cites W1970293798 @default.
• W1947892387 cites W1983268040 @default.
• W1947892387 cites W1983541797 @default.
• W1947892387 cites W1989261293 @default.
• W1947892387 cites W1995499476 @default.
• W1947892387 cites W1995755401 @default.
• W1947892387 cites W1997027978 @default.
• W1947892387 cites W1999307856 @default.
• W1947892387 cites W2000539788 @default.
• W1947892387 cites W2002187143 @default.
• W1947892387 cites W2014219101 @default.
• W1947892387 cites W2016411107 @default.
• W1947892387 cites W2027534206 @default.
• W1947892387 cites W2030583109 @default.
• W1947892387 cites W2034919108 @default.
• W1947892387 cites W2035309658 @default.
• W1947892387 cites W2039355845 @default.
• W1947892387 cites W2050273631 @default.
• W1947892387 cites W2052605451 @default.
• W1947892387 cites W2057357407 @default.
• W1947892387 cites W2059974837 @default.
• W1947892387 cites W2061994521 @default.
• W1947892387 cites W2069951758 @default.
• W1947892387 cites W2077589642 @default.
• W1947892387 cites W2082812095 @default.
• W1947892387 cites W2087193732 @default.
• W1947892387 cites W2123819897 @default.
• W1947892387 cites W2125053662 @default.
• W1947892387 cites W2126299335 @default.
• W1947892387 cites W2139162201 @default.
• W1947892387 cites W2145430737 @default.
• W1947892387 cites W2161534884 @default.
• W1947892387 cites W2167031471 @default.
• W1947892387 cites W2171028772 @default.
• W1947892387 cites W2277498060 @default.
• W1947892387 cites W4212936249 @default.
• W1947892387 cites W4232534952 @default.
• W1947892387 doi "https://doi.org/10.1242/jcs.114.21.3813" @default.
• W1947892387 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11719548" @default.
• W1947892387 hasPublicationYear "2001" @default.
• W1947892387 type Work @default.
• W1947892387 sameAs 1947892387 @default.
• W1947892387 citedByCount "65" @default.
• W1947892387 countsByYear W19478923872012 @default.
• W1947892387 countsByYear W19478923872013 @default.
• W1947892387 countsByYear W19478923872014 @default.
• W1947892387 countsByYear W19478923872015 @default.
• W1947892387 countsByYear W19478923872016 @default.
• W1947892387 countsByYear W19478923872017 @default.
• W1947892387 countsByYear W19478923872018 @default.
• W1947892387 countsByYear W19478923872019 @default.
• W1947892387 countsByYear W19478923872020 @default.
• W1947892387 countsByYear W19478923872021 @default.
• W1947892387 countsByYear W19478923872022 @default.
• W1947892387 crossrefType "journal-article" @default.
• W1947892387 hasAuthorship W1947892387A5001954776 @default.
• W1947892387 hasAuthorship W1947892387A5003537474 @default.
• W1947892387 hasAuthorship W1947892387A5015148582 @default.
• W1947892387 hasAuthorship W1947892387A5023575597 @default.
• W1947892387 hasAuthorship W1947892387A5060926236 @default.
• W1947892387 hasConcept C105702510 @default.
• W1947892387 hasConcept C109523444 @default.
• W1947892387 hasConcept C142724271 @default.
• W1947892387 hasConcept C153911025 @default.
• W1947892387 hasConcept C197534660 @default.
• W1947892387 hasConcept C202751555 @default.
• W1947892387 hasConcept C203014093 @default.
• W1947892387 hasConcept C204787440 @default.
• W1947892387 hasConcept C2776164576 @default.
• W1947892387 hasConcept C2776232661 @default.
• W1947892387 hasConcept C2776914184 @default.
• W1947892387 hasConcept C2779244956 @default.
• W1947892387 hasConcept C2780550940 @default.
• W1947892387 hasConcept C2781184567 @default.
• W1947892387 hasConcept C2781403057 @default.
• W1947892387 hasConcept C3020332539 @default.
• W1947892387 hasConcept C55493867 @default.
• W1947892387 hasConcept C71924100 @default.
• W1947892387 hasConcept C86803240 @default.
• W1947892387 hasConcept C95444343 @default.
• W1947892387 hasConceptScore W1947892387C105702510 @default.
• W1947892387 hasConceptScore W1947892387C109523444 @default.
• W1947892387 hasConceptScore W1947892387C142724271 @default.
• W1947892387 hasConceptScore W1947892387C153911025 @default.

• next